Effects of Danshensu and Salvianolic Acid B from Salvia miltiorrhiza Bunge (Lamiaceae) on Cell Proliferation and Collagen and Melanin Production

Abstract

Danshensu (DSU) and salvianolic acid B (SAB) are the primary water-soluble compounds of Salvia miltiorrhiza Bunge (Lamiaceae). In this study, we analyzed the effects of DSU, SAB and a S. miltiorrhiza extract (SME) on cell proliferation. Additionally, the effects of DSU and SAB on collagen synthesis in Detroit 551 human normal fibroblast cells and on melanin production in B16 melanoma cells were verified. The results demonstrated that SME can enhance the proliferation of Detroit 551 cells and that this boost may be caused by DSU and SAB. This research showed that SME, DSU and SAB all have the ability to increase the production of collagen in Detroit 551 cells. The results also confirmed that DSU and SAB can attenuate the α-MSH-stimulated melanin production of B16 cells by inhibiting tyrosinase activity. Therefore, SME, DSU and SAB each have the potential to be utilized as active ingredients in wound healing or cosmetic treatments. In the future, DSU and SAB could also be used as functional components for treating hyperpigmentation.

Anticancer Effects of Salvia miltiorrhiza Alcohol Extract on Oral Squamous Carcinoma Cells

“…Many studies of Danshen's antitumor potential have produced significant findings. Active components of Danshen include danshensu, tanshinones, and salvianolic acids, all of which have been shown to exert antioxidant, antimicrobial, anti-inflammatory, anticancer, and cardiovascular-protective effects [ 27 – 29 ]. These clinical effects are generally attributed to two major Danshen components: tanshinone IIA (Tan-IIA) and salvianolic acid B (Sal-B) [ 29 – 31 ].…”

Hair Dyes Resorcinol and Lawsone Reduce Production of Melanin in Melanoma Cells by Tyrosinase Activity Inhibition and Decreasing Tyrosinase and Microphthalmia-Associated Transcription Factor (MITF) Expression

“…The cells were sustained in a humidified incubator with 5% CO 2 at 37 °C. Every three to four days, the cells were sub-cultured to maintain logarithmic growth [ 27 ].…”

Establishment of a melanogenesis regulation assay system using a fluorescent protein reporter combined with the promoters for the melanogenesis-related genes in human melanoma cells

“…The cell-based melanin content assay in mouse melanocytes (e.g., B16F10 mouse melanoma cells) is better than the mushroom tyrosinase activity assay because it provides more credible results http://dx.doi.org/10.1016/j.enzmictec.2014.09.008 0141-0229/© 2014 Elsevier Inc. All rights reserved. [13,14]. However, this assay is time-consuming, requiring approximately 3-5 days to complete.…”