Establishment of a melanogenesis regulation assay system using a fluorescent protein reporter combined with the promoters for the melanogenesis-related genes in human melanoma cells

Lin, Chih-Chien; Yang, Chao‐Hsun; Lin, Ying-Ju; Chiu, Yu‐Han; Chen, Cheng-Yu

doi:10.1016/j.enzmictec.2014.09.008

Cited by 11 publications

(6 citation statements)

References 34 publications

(38 reference statements)

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“…Braasch et al [43] have reported that TYR is the rate-limiting enzyme in the process of producing melanin and TYRP1 functions in the preservation of TYR catalytic activity [44,45]. TYRP2 encoded dopachrome, tautomerase, and catalyzed the synthesis of 5,6-dihydroxyindole-2-carboxylate from dopachrome [46][47][48]. Our results provided more evidence for the melanin synthesis and further showed the importance of tyrosine family genes in the black body color formation process.…”

Section: Discussionsupporting

confidence: 63%

Identification of Genes and SNPs Related to Body Colors by Transcriptome Profiling in Leopard Coral Grouper (Plectropomus leopardus Lacépède)

Yuan¹,

Sun²,

Zhu

et al. 2023

Journal of Applied Ichthyology

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Body colors are an important trait for the survival and reproduction of animals. In this study, transcriptomes and related single nucleotide polymorphisms (SNPs) of black- and red-colored Plectropomus leopardus (Lacépède) were identified to investigate important molecules and SNP markers associated with body color formation. In the comparison of red- and black-colored groups, 489 differential expressed genes (DEGs) were detected and among them, 236 up- and 253 down-regulated genes were found in the red-colored group. Gene ontology (GO) analysis of DEGs showed that pigmentation associated terms including pigment granule, pigmentation, melanin metabolic process, tyrosine metabolic process, pigment metabolic process, and pigmentation cell differentiation were enriched. Kyoto encyclopedia of genes and genomes (KEGG) analysis of DEGs presented that melanin related pathways (tyrosine metabolism and melanogenesis) were enriched. Furthermore, lipid metabolism and immune-related metabolism were also enriched. A total of 1,048,575 SNPs were detected from the transcriptome, and among them, 627,172 SNPs were located in the gene region. A total of 1323 SNPs were identified as differential SNPs in the comparison of black- and red-colored groups and were located in 1127 genes. Among the 1127 genes, 20 genes were identified as DEGs and were comprised of 9 SNPs with a potential role in body color selective breeding in fish farming.

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Section: Discussionsupporting

confidence: 63%

Identification of Genes and SNPs Related to Body Colors by Transcriptome Profiling in Leopard Coral Grouper (Plectropomus leopardus Lacépède)

Yuan¹,

Sun²,

Zhu

et al. 2023

Journal of Applied Ichthyology

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“…The knockdown of TYRP1 in zebrafish and medaka results in the formation of brown instead of black eumelanin, accompanied by severe melanosome defects, and revealing the importance of TYRP1 in melanin (Braasch et al, 2009b). DCT is also expressed exclusively in melanophores and melanoblasts and encodes DCT, which functions in the catalysis of dopachrome into 5, 6-Dihydroxyindole-2-carboxylate (Lin et al, 2015). DCT is a key gene in salmonid melanogenesis and its knockout results in a diluted color phenotype (Sivka et al, 2013).…”

Section: Black Melanin Colorationmentioning

confidence: 99%

Integrative Transcriptomics and Metabolomics Analysis of Body Color Formation in the Leopard Coral Grouper (Plectropomus leopardus)

et al. 2021

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Body color is an important economic and ecological trait in aquatic animals, which influence their economic values and determine the animal ability to survive in the marine environment. Red-colored Plectropomus leopardus is in high demand owing to its bright color and potential value for artificial breeding. High-throughput transcriptome sequencing and liquid chromatography tandem-mass spectrometry (LC-MS/MS) approaches were used to identify transcript and metabolic differences between black-colored and red-colored P. leopardus. Compared with the black-colored group, 218 and 181 differentially expressed genes were up- and down- regulated, respectively, in the red-colored group. 425 and 56 significantly different metabolites were identified in LC-MS positive and LC-MS negative ion models, respectively, between two colored groups. Based on the integrative analysis, the red-colored group exhibited greater carotenoid uptake, transport, and accumulation activity potential than those in the black-colored group and may consume more arachidonic acid for body color formation. The black-colored group showed greater melanin synthesis activity compared with the red-colored group. These results substantially improve the understanding of molecular and metabolic mechanisms underlying body color formation in P. leopardus and the potential of body color for the environment adaptation.

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“…The fluorescence microscope is mainly used to record the images, and the ELISA microplate reader can analyze the fluorescent intensity. The measured intensity percentages are expressed as the relative fluorescent intensity [26].…”

Section: Fluorescent Protein Intensity Measurementmentioning

confidence: 99%

Human Microfibrillar-Associated Protein 4 (MFAP4) Gene Promoter: A TATA-Less Promoter That Is Regulated by Retinol and Coenzyme Q10 in Human Fibroblast Cells

Lin

Chen

Yin

et al. 2020

IJMS

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Elastic fibers are one of the major structural components of the extracellular matrix (ECM) in human connective tissues. Among these fibers, microfibrillar-associated protein 4 (MFAP4) is one of the most important microfibril-associated glycoproteins. MFAP4 has been found to bind with elastin microfibrils and interact directly with fibrillin-1, and then aid in elastic fiber formation. However, the regulations of the human MFAP4 gene are not so clear. Therefore, in this study, we firstly aimed to analyze and identify the promoter region of the human MFAP4 gene. The results indicate that the human MFAP4 promoter is a TATA-less promoter with tissue- and species-specific properties. Moreover, the promoter can be up-regulated by retinol and coenzyme Q10 (coQ10) in Detroit 551 cells.

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Establishment of a melanogenesis regulation assay system using a fluorescent protein reporter combined with the promoters for the melanogenesis-related genes in human melanoma cells

Cited by 11 publications

References 34 publications

Identification of Genes and SNPs Related to Body Colors by Transcriptome Profiling in Leopard Coral Grouper (Plectropomus leopardus Lacépède)

Identification of Genes and SNPs Related to Body Colors by Transcriptome Profiling in Leopard Coral Grouper (Plectropomus leopardus Lacépède)

Integrative Transcriptomics and Metabolomics Analysis of Body Color Formation in the Leopard Coral Grouper (Plectropomus leopardus)

Human Microfibrillar-Associated Protein 4 (MFAP4) Gene Promoter: A TATA-Less Promoter That Is Regulated by Retinol and Coenzyme Q10 in Human Fibroblast Cells

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