Effects of Decavanadate Salts with Organic and Inorganic Cations on Escherichia coli, Giardia intestinalis, and Vero Cells

Abstract

Decavanadate salts with nicotinamide (3-pyridinecarboxamide, 3-pca) and isonicotinamide (4-pyridinecarboxamide, 4-pca) in both neutral and protonated forms, (3-Hpca)[HVO]·2HO·2(3-pca) (complex I) and (4-Hpca)[HVO]·2(4-pca) (complex II), have been synthesized and characterized by vibrational spectroscopy (infrared and Raman), thermogravimetric analysis (TGA), V NMR, and single-crystal X-ray diffraction analysis. The effects of sodium decavanadate (henceforth called NaV) and compounds I and II on Escherichia coli, Giardia intestinalis, and Vero (African green monkey epithelial kidney) cells were evaluated. Enhanced growth inhibitory activity against E. coli cultures was observed upon treatment with products I and II when compared to that with NaV (GI values of 2.8, 4.0, and 11 mmol L, respectively), as well as lower cell viability as measured by the intake of propidium iodide (PI). Exposure of Giardia trophozoites to NaV and II revealed reduction in trophozoite viability (GI values of ca. 10 μmol L) and affected the parasite adherence to both polystyrene culture tubes and a monolayer of Vero cells, even at low concentrations. A lesser effect on Giardia was shown for I. Furthermore, all three compounds were significantly less toxic to Vero cells than the reference drug, albendazole, employed in the treatment of giardiasis. Toxicity reports of oxidovanadium compounds toward Giardia are unprecedented and open a path to the development of new therapeutic agents.

Kinetic Studies of Sodium and Metforminium Decavanadates Decomposition and In Vitro Cytotoxicity and Insulin- Like Activity

“…The three vanadium and Metf compounds do not affect the viability of the HEK293 cells; this is an important result from this work, which may have an impact due to the new strategies intended to reduce the renal toxicity induced by cisplatin [44,45]. [48]. However, in the three studies presented before [46][47][48] for decavanadate compounds, the effect of the counter ion in the cytotoxic studies was not studied.…”

“…[48]. However, in the three studies presented before [46][47][48] for decavanadate compounds, the effect of the counter ion in the cytotoxic studies was not studied.…”

Peptidylarginine Deiminase Inhibition Abolishes the Production of Large Extracellular Vesicles From Giardia intestinalis, Affecting Host-Pathogen Interactions by Hindering Adhesion to Host Cells

“…After 2 min, the wells were exhaustively washed with 200 μL of PBS (1x). Elution was made with a sodium citrate solution (0.05 μmol, 10 min), and absorbance was determined at 540 nm on a plate spectrophotometer, as described in Missina et al ( 2018 ).…”

Crystal engineering of an adenine–decavanadate molecular device towards label-free chemical sensing and biological screening

“…Ohlin, 2014). Decavanadate and its compounds were known for their insulin mimetic behavior for years but recently several papers have been published that demonstrate a variety of biological activities including anticancer, antibacterial and antivirus characteristics (Missina et al, 2018;Samart et al, 2018;Shah et al, 2014;Li et al, 2010).…”