Effects of Defaunation on Fermentation Characteristics and Methane Production by Rumen Microbes In vitro When Incubated with Starchy Feed Sources

Qin, Wei-Ze; Li, C.Y.; Kim, J.K.; Ju, J.G.; Song, M.K.

doi:10.5713/ajas.2012.12240

Cited by 24 publications

(25 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1). This finding is consistent with that of a previous study of the effects of defaunation on the fermentation characteristics of starchy feed sources, which revealed that rumen ciliates were eliminated by adding SDS after 1 h of incubation [18]. Surfactants react with cholesterol in eukaryotic cell membranes [19], which alter membrane fluidity and permeability via increased unsaturation of lipids [20,21].…”

Section: Effect Of Sds Addition On Pretreatment With Rumen Fluidsupporting

confidence: 92%

Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition

Takizawa

Baba

Tada

et al. 2020

J Mater Cycles Waste Manag

View full text Add to dashboard Cite

Rumen fluid has been applied to lignocellulosic biomass digest for methane production, and various feed supplements have been shown to improve ruminant digestion of lignocellulosic biomass. Therefore, we investigated the effects of sodium dodecyl sulfate (SDS) on the pretreatment of lignocellulosic biomass with rumen fluid and subsequent methane fermentation. SDS was mixed with rumen fluid at concentrations of 0.1, 0.2, 0.4, and 0.8 g/L. After SDS addition, the waste paper was pretreated with rumen fluid at 37 °C for 6 h. SDS addition decreased the number of surviving rumen ciliates after pretreatment. SDS addition increased the dissolved chemical oxygen demand during pretreatment; however, SDS addition did not increase the volatile fatty acid concentration. After pretreatment, batch methane fermentation of pretreated waste paper was performed at 35 °C for 45 days. SDS addition at 0.1 and 0.2 g/L shortened the waste paper digestion time and enhanced methane gas production compared to the control. By contrast, SDS addition at 0.4 and 0.8 g/L remarkably inhibited methane production from waste paper. These findings suggest that low concentrations of SDS can improve the efficiency of lignocellulosic biomass pretreatment with rumen fluid, and can enhance methane production from waste paper.

show abstract

Section: Effect Of Sds Addition On Pretreatment With Rumen Fluidsupporting

confidence: 92%

Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition

Takizawa

Baba

Tada

et al. 2020

J Mater Cycles Waste Manag

View full text Add to dashboard Cite

show abstract

“…Saponin is known to inhibit the growth of acetate-producing bacteria (e.g., Butyrivibrio fibrisolvens ) and reduce the population of protozoa by reacting with cholesterol in the membrane of protozoa in the rumen [21], causing protozoa to rupture [22]. Protozoa elimination through defaunation can reduce the ratio of acetate to propionate [22,23,24]. This may be one of the reasons why a 4.5% licorice root extract supplementation reduced the ratio of acetate to propionate in the present study.…”

Section: Discussionmentioning

confidence: 99%

Effects of Licorice Extract Supplementation on Feed Intake, Digestion, Rumen Function, Blood Indices and Live Weight Gain of Karakul Sheep

Guo

Cheng

Liu

et al. 2019

Animals

View full text Add to dashboard Cite

This study was designed to investigate the effects of licorice extract supplemented to a cottonseed hull-based diet on rumen function, blood indices and growth of Karakul sheep. Twelve rumen-fistulated 1.5-year-old sheep were blocked in pairs by live weight. Sheep within pairs were randomly allocated to feed either on a cottonseed hull basal diet (control group) or on a basal diet containing 4.5% of licorice extract on dry matter (DM) basis (supplemented group). Sheep were housed individually and fed for 60 days, with a 15-day adaptation period and a 45-day measurement period. Feed intake and live weight gain were quantified. Rumen and blood samples were taken during the measurement period. The DM intake was lower for the supplemented group than for the control group. The mean ruminal concentrations of total volatile fatty acid (VFA) and acetate and the ratio of acetate to propionate were lower, while the levels of propionate and butyrate were higher for the supplemented group than for the control group. Average daily live weight gain, digestible energy intake, apparent nutrient digestibility and feed conversion efficiency did not differ between the two treatments. The serum concentrations of immunoglobulin A and G were 2.1 and 1.8 times greater, and total antioxidant and superoxide dismutase increased by 1.8 and 1.2 times in the supplemented group compared with the control group. These results indicated that licorice extract supplementation in the diet at 4.5% of DM had a limited impact on rumen function but improved blood immunoglobulin and anti-oxidative status of Karakul sheep, without impairment of feed conversion efficiency or live weight gain.

show abstract

“…In the present study, those three inhibitors at the above concentrations were further evaluated with rumen fluid containing all the diverse protozoal and other microbial species typically found in the rumen of dairy cows. SDS, which was effective in achieving in vitro defaunation of rumen microbiota (Qin et al, 2012), was included at 1.44 mmol/L as a positive control for defaunation. A stock solution of each inhibitor was prepared aseptically in water, except for PMSF, which is insoluble in water, that was dissolved in absolute ethanol.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of Rumen Protozoa by Specific Inhibitors of Lysozyme and Peptidases in vitro

Park

Mao

2019

Front. Microbiol.

View full text Add to dashboard Cite

Defaunation studies have shown that rumen protozoa are one of the main causes of low nitrogen utilization efficiency due to their bacterivory and subsequent intraruminal cycling of microbial protein in ruminants. In genomic and transcriptomic studies, we found that rumen protozoa expressed lysozymes and peptidases at high levels. We hypothesized that specific inhibition of lysozyme and peptidases could reduce the activity and growth of rumen protozoa, which can decrease their predation of microbes and proteolysis and subsequent ammoniagenesis by rumen microbiota. To test the above hypothesis, we evaluated three specific inhibitors: imidazole (IMI), a lysozyme inhibitor; phenylmethylsulphonyl fluoride (PMSF), a serine protease inhibitor; and iodoacetamide (IOD), a cysteine protease inhibitor; both individually and in combinations, with sodium dodecyl sulfate (SDS) as a positive control. Rumen fluid was collected from two Jersey dairy cows fed either a concentrate-based dairy ration or only alfalfa hay. Each protozoa-enriched rumen fluid was incubated for 24 h with or without the aforementioned inhibitors and fed a mixture of ground wheat grain, alfalfa, and grass hays to support microbial growth. Live protozoa cells were morphologically identified and counted simultaneously at 3, 6, 12, and 24 h of incubation. Fermentation characteristics and prokaryotic composition were determined and compared at the end of the incubation. Except for IOD, all the inhibitors reduced all the nine protozoal genera identified, but to different extents, in a time-dependent manner. IOD was the least inhibitory to protozoa, but it lowered ammoniagenesis the most while not decreasing feed digestibility or concentration of volatile fatty acids (VFA). ANCOM analysis identified loss of Fibrobacter and overgrowth of Treponema, Streptococcus, and Succinivibrio in several inhibitor treatments. Functional prediction (from 16S rRNA gene amplicon sequences) using the CowPI database showed that the inhibitors decreased the relative abundance of the genes encoding amino acid metabolism, especially peptidases, and lysosome in the rumen microbiota. Overall, inhibition of protozoa resulted in alteration of prokaryotic microbiota and in vitro fermentation, and peptidases, especially cysteine-peptidase, may be targeted to improve nitrogen utilization in ruminants.

show abstract

Effects of Defaunation on Fermentation Characteristics and Methane Production by Rumen Microbes In vitro When Incubated with Starchy Feed Sources

Cited by 24 publications

References 45 publications

Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition

Sodium dodecyl sulfate improves the treatment of waste paper with rumen fluid at lower concentration but decreases at higher condition

Effects of Licorice Extract Supplementation on Feed Intake, Digestion, Rumen Function, Blood Indices and Live Weight Gain of Karakul Sheep

Inhibition of Rumen Protozoa by Specific Inhibitors of Lysozyme and Peptidases in vitro

Contact Info

Product

Resources

About