Effects of Dermatopontin gene silencing on apoptosis and proliferation of osteosarcoma MG‑63 cells

Abstract

The present study aimed to investigate the effect of Dermatopontin (DPT) gene silencing on the apoptosis and proliferation of osteosarcoma MG‑63 cells. Three eukaryotic expression vectors of short hairpin (sh)RNA fragments targeting different loci of DPT were designed and transfected into an osteosarcoma cell line MG‑63. The cells were assigned to a blank, shRNA‑control, DPT‑shRNA‑a, DPT‑shRNA‑b or DPT‑shRNA‑c group. The shRNA with the highest silencing efficiency was screened using reverse transcription‑quantitative polymerase chain reaction and western blotting. The screened shRNA was transfected into MG‑63 cells. The proliferation, cell cycle and apoptosis of MG‑63 cells were measured using a Cell Counting Kit‑8 assay, flow cytometry and Annexin V‑fluorescein isothiocyanate assay. The recombinant plasmids containing DPT shRNA were successfully constructed. DPT gene silencing was able to significantly reduce the proliferation rate of MG‑63 cells (P<0.05). The proportion of cells in the G0/G1 phase and in the G2/M phase increased significantly (both P<0.05), while the proportion of cells in the S phase decreased (P<0.05). Furthermore, the cell apoptosis rate increased significantly (P<0.05). These results demonstrate that DPT gene silencing is able to reduce the proliferation of MG‑63 cells, slow down cell cycle progression and promote apoptosis, hence may become a novel target for the treatment of osteosarcoma.

Identifying patients with rapid progression from hormone-sensitive to castration-resistant prostate cancer: a retrospective study

“…In terms of the five proteins in the model, ARHGEF1 and DPT have been proved to promote the occurrence and progression of other cancers [26][27][28] , which were consistent with our results. ANAPC4 and CMAS have not been reported in cancer.…”

Cellular Transcriptomics of Carboplatin Resistance in a Metastatic Canine Osteosarcoma Cell Line

“…The upregulation of FSIP1 (fibrous sheath interacting protein 1) correlates with poor prognosis in breast cancer and FSIP1 knockout in a mouse model has been found to improve docetaxel sensitivity [ 85 ]. DPT (dermatopontin) promotes cellular adhesion and enhances TGFB1 during the process of wound repair [ 86 , 87 ]. OMD (osteomodulin) is involved in osteoblast differentiation and OGN (osteoglycin) regulates bone and glucose homeostasis and has been indicated as a tumor suppressor [ 88 , 89 ].…”

Proteomic Analysis of Aqueous Humor Reveals Changes in Extracellular Matrix Pathways in Proliferative Diabetic Retinopathy Patients Treated with Intravitreal Ranibizumab

“…What's more, DPT protein, also known as tyrosine-rich acidic matrix protein (TRAMP), is predominantly found in the dermis of the skin and is an extensively distributed noncollagenous component of the ECM (Kim et al, 2019). It is involved in cell adhesion (Kramer et al, 2017), fibrosis (Wu et al, 2014), myogenesis (Kim et al, 2019), angiogenesis (Krishnaswamy et al, 2017), adipose tissue inflammation (Catalán et al, 2022), osteogenic proliferation and differentiation (Xi et al, 2018;Zhao et al, 2023), and tumor invasion and metastasis (Yamatoji et al, 2012). Combining DIA analysis and existing reports, we focused on the protein of DPT.…”