Effects of detyrosinated tubulin on Na<sup>+</sup>,K<sup>+</sup>‐ATPase activity and erythrocyte function in hypertensive subjects

Amaiden, Marina R.; Santander, Verónica S.; Monesterolo, Noelia E.; Nigra, Ayelén D.; Rivelli, Juan F.; Campetelli, Alexis N.; Pié, Juan; Casale, César H.

doi:10.1016/j.febslet.2014.12.022

Cited by 16 publications

(20 citation statements)

References 38 publications

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“…Touyz and Milne stated that Na + /K + -ATPase, Mg 2+ -ATPase, and Ca 2+ -ATPase activities of erythrocyte membrane were downregulated in hypertensive patients [53]. And further studies found that the inhibited ATPase activities suppress the cation transports, inducing hypertension [24]. In our experiment, we deduced that AlCl 3 disturbs the function of erythrocyte membrane by decreasing the activities of Na + /K + -ATPase, Mg 2+ -ATPase, and Ca 2+ -ATPase, and consequently, induces hypertension.…”

Section: Discussionmentioning

confidence: 98%

“…The abnormalities in sodium transport of erythrocyte membrane were found in patients with essential hypertension [21,22]. Further research showed that the activity of membrane-bound (Na + /K + )-ATPase was downregulated in hypertensive patients [23], and the inhibition of ATPase activity disturbed ion transport of membrane, thus, leading to an increase in blood pressure [24]. Kaczmarska et al proved that disturbed membrane protein and impaired oxygen release of erythrocyte membrane contributed to the development of hypertension [25].…”

Section: Introductionmentioning

confidence: 99%

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Aluminum Trichloride Induces Hypertension and Disturbs the Function of Erythrocyte Membrane in Male Rats

Zhang

Cao

Sun

et al. 2015

Biol Trace Elem Res

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Aluminum (Al) is the most abundant metal in the earth's crust. Al accumulates in erythrocyte and causes toxicity on erythrocyte membrane. The dysfunction of erythrocyte membrane is a potential risk to hypertension. The high Al content in plasma was associated with hypertension. To investigate the effect of AlCl3 on blood pressure and the function of erythrocyte membrane, the rats were intragastrically exposed to 0, 64(1/20 LD50), 128(1/10 LD50), and 256(1/5 LD50) mg/kg body weight AlCl3 in double distilled water for 120 days, respectively. Then, we determined the systolic and mean arterial blood pressures of rats, the osmotic fragility, the percentage of membrane proteins, the activities of Na(+)/K(+)-ATPase, Mg(2+)-ATPase, Ca(2+)-ATPase, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-pX), and malondialdehyde (MDA) content of the erythrocyte membrane in this experiment. The results showed that AlCl3 elevated the systolic and mean arterial blood pressure of rats, increased the osmotic fragility, decreased the percentage of membrane protein, inhibited the activities of Na(+)/K(+)-ATPase, Mg(2+)-ATPase, Ca(2+)-ATPase, CAT, SOD and GSH-pX, and increased the MDA content of erythrocyte membrane. These results indicate that AlCl3 may induce hypertension by disturbing the function of erythrocyte membrane.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Aluminum Trichloride Induces Hypertension and Disturbs the Function of Erythrocyte Membrane in Male Rats

Zhang

Cao

Sun

et al. 2015

Biol Trace Elem Res

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“…In hypertension, this mechanism is important because the decrease in the content and the enzymatic activity of TTL in hypertensive erythrocytes (Amaiden et al, 2015) increases the level of detyrosinated tubulin, which has an inhibitory effect on NKA activity, according to the mentioned results. This would explain why the cells of hypertensive individuals accumulate Na + .…”

Section: Perspectivesmentioning

confidence: 90%

“…In addition, the regulation of Na + /K + homeostasis, also dependent on the tubulin–NKA interaction, opens new perspectives in different pathologies in which the homeostasis of these ions is unbalanced. In hypertension, this mechanism is important because the decrease in the content and the enzymatic activity of TTL in hypertensive erythrocytes (Amaiden et al, ) increases the level of detyrosinated tubulin, which has an inhibitory effect on NKA activity, according to the mentioned results. This would explain why the cells of hypertensive individuals accumulate Na + .…”

Section: Perspectivesmentioning

confidence: 96%

Tubulin–Na⁺_,K⁺‐ATPase interaction: Involvement in enzymatic regulation and cellular function

Santander

Campetelli

Monesterolo

et al. 2018

Journal Cellular Physiology

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A new function for tubulin was described by our laboratory: acetylated tubulin forms a complex with Na+,K +‐ATPase (NKA) and inhibits its activity. This process was shown to be a regulatory factor of physiological importance in cultured cells, human erythrocytes, and several rat tissues. Formation of the acetylated tubulin–NKA complex is reversible. We demonstrated that in cultured cells, high concentrations of glucose induce translocation of acetylated tubulin from cytoplasm to plasma membrane with a consequent inhibition of NKA activity. This effect is reversed by adding glutamate, which is coctransported to the cell with Na +. Another posttranslational modification of tubulin, detyrosinated tubulin, is also involved in the regulation of NKA activity: it enhances the NKA inhibition induced by acetylated tubulin. Manipulation of the content of these modifications of tubulin could work as a new strategy to maintain homeostasis of Na + and K +, and to regulate a variety of functions in which NKA is involved, such as osmotic fragility and deformability of human erythrocytes. The results summarized in this review show that the interaction between tubulin and NKA plays an important role in cellular physiology, both in the regulation of Na +/K + homeostasis and in the rheological properties of the cells, which is mechanically different from other roles reported up to now.

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“…In hypertensive patients the level of detyrosinated tubulin was higher in the sedimentable fraction. Consequent translocation of detyrosinated tubulin to the plasma membrane enhanced the inhibitory effect of tubulin on Na,K-ATPase activity resulting in reduced RBC deformability (Amaiden et al 2015).…”

Section: Hypertensionmentioning

confidence: 99%

The Role of Red Blood Cell Deformability and Na,K-ATPase Function in Selected Risk Factors of Cardiovascular Diseases in Humans: Focus on Hypertension, Diabetes Mellitus and Hypercholesterolemia

Radošinská

Vrbjar

2016

Physiol Res

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Deformability of red blood cells (RBC) is the ability of RBC to change their shape in order to pass through narrow capillaries in circulation. Deterioration in deformability of RBC contributes to alterations in microcirculatory blood flow and delivery of oxygen to tissues. Several factors are responsible for maintenance of RBC deformability. One of them is the Na,K-ATPase known as crucial enzyme in maintenance of intracellular ionic homeostasis affecting thus regulation of cellular volume and consequently RBC deformability. Decreased deformability of RBC has been found to be the marker of adverse outcomes in cardiovascular diseases (CVD) and the presence of cardiovascular risk factors influences rheological properties of the blood. This review summarizes knowledge concerning the RBC deformability in connection with selected risk factors of CVD, including hypertension, hyperlipidemia, and diabetes mellitus, based exclusively on papers from human studies. We attempted to provide an update on important issues regarding the role of Na,K-ATPase in RBC deformability. In patients suffering from hypertension as well as diabetes mellitus the Na,K-ATPase appears to be responsible for the changes leading to alterations in RBC deformability. The triggering factor for changes of RBC deformability during hypercholesterolemia seems to be the increased content of cholesterol in erythrocyte membranes.

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Effects of detyrosinated tubulin on Na⁺,K⁺‐ATPase activity and erythrocyte function in hypertensive subjects

Cited by 16 publications

References 38 publications

Aluminum Trichloride Induces Hypertension and Disturbs the Function of Erythrocyte Membrane in Male Rats

Aluminum Trichloride Induces Hypertension and Disturbs the Function of Erythrocyte Membrane in Male Rats

Tubulin–Na⁺_,K⁺‐ATPase interaction: Involvement in enzymatic regulation and cellular function

The Role of Red Blood Cell Deformability and Na,K-ATPase Function in Selected Risk Factors of Cardiovascular Diseases in Humans: Focus on Hypertension, Diabetes Mellitus and Hypercholesterolemia

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Effects of detyrosinated tubulin on Na+,K+‐ATPase activity and erythrocyte function in hypertensive subjects

Cited by 16 publications

References 38 publications

Aluminum Trichloride Induces Hypertension and Disturbs the Function of Erythrocyte Membrane in Male Rats

Aluminum Trichloride Induces Hypertension and Disturbs the Function of Erythrocyte Membrane in Male Rats

Tubulin–Na+,K+‐ATPase interaction: Involvement in enzymatic regulation and cellular function

The Role of Red Blood Cell Deformability and Na,K-ATPase Function in Selected Risk Factors of Cardiovascular Diseases in Humans: Focus on Hypertension, Diabetes Mellitus and Hypercholesterolemia

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Product

Resources

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Effects of detyrosinated tubulin on Na⁺,K⁺‐ATPase activity and erythrocyte function in hypertensive subjects

Tubulin–Na⁺_,K⁺‐ATPase interaction: Involvement in enzymatic regulation and cellular function