Effects of diet on the lipid composition of Echinostomacaproni (Trematoda) in ICR mice

Abstract

High-performance thin-layer chromatography (HPTLC) was used to determine neutral lipids and phospholipids in the intestinal trematode Echinostoma caproni from experimentally infected ICR mice fed a high-fat diet (hen's egg yolk) as compared with worms from mice fed a standard laboratory diet. Worms were removed from the hosts at 2, 3, and 4 weeks postinfection (p.i.). Analysis by TLC-densitometry showed significantly greater amounts of triacylglycerols and free sterols at 2, 3, and 4 weeks p.i. in worms from mice on the high-fat diet as compared with worms from mice on the standard laboratory diet. Significantly greater amounts of phosphatidylcholine and phosphatidylethanolamine were found in worms from mice on the high-fat diet as compared with worms from those on the standard diet at 2 weeks p.i. but not at 3 and 4 weeks p.i. The results of this study suggest that the host diet influences the lipid content of E. caproni adults.

Effects of tonicity on the release of neutral lipids in Echinostoma caproni adults and observations on lipids in excysted metacercariae

“…In addition to studies on the lipophilic substances released into these media, observations were also made on the neutral lipid content of the worms. Previous observations on the neutral lipid content of E. caproni adults have been presented by Frazer et al (1997) and Lee et al (1998). Marsit et al (2000) examined lipids in encysted metacercariae of E. caproni.…”

Light and confocal microscopic observations of adultSchistosoma mansonifrom mice fed on a high-fat diet

“…For example, a vitamin B-deficient diet and a protein-free diet had a detrimental effect on the growth of Echinostoma caproni in ICR mice (Rosario & Fried, 1999; Simpkins & Fried, 2001), and E. trivolvis developed poorly in avian hosts lacking fat-soluble vitamins (Simpkins & Fried, 1999). Significantly greater amounts of phosphatidylcholine and phosphatidylethanolamine were found in worms from mice maintained on a high-fat diet as compared with worms from those on a standard diet, suggesting that the host diet influences the lipid content of adults of E. caproni (Frazer et al , 1997).…”

Lipids

“…The major phospholipids were phosphatidylcholine (0.45%) and phosphatylidylethanolamine (0.34%). In a companion study, Frazer et al (167) used HPTLC to identify neutral lipids in the intestinal trematode Echinostoma caproni from experimentally infected ICR mice fed a high fat diet of hen's egg yolk compared with worms from mice fed a standard laboratory diet. Significantly greater amounts of phosphatidylcholine and phosphatidylethanolamine were found in worms from mice on the high fat diet at 2 weeks post-infection.…”

“…Frazer et al (166) used silica gel HPTLC to identify neutral lipids and phospholipids in the freshwater snail Lymnaea elodes. The mobile phase for neutral lipids was petroleum ether-diethyl ether-acetic acid (40:20:1), and zones were detected with 5% ethanolic phosphomolybdic acid; quantification was by densitometry at 700 nm.…”