Effects of tonicity on the release of neutral lipids in Echinostoma caproni adults and observations on lipids in excysted metacercariae

Schneck, Jessica L.; Fried, Bernard; Sherma, Joseph

doi:10.1007/s00436-003-1051-y

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…In mice harboring a 2-week-old E. caproni -infection, an increased breakdown of membrane lipids in the host intestinal tissue has been observed [35], which is consistent with the present findings of a maximum lipid increase on day 12 post-infection [36]. The excretory products of E. caproni in the intestinal mucosa are primarily free sterols, triaglycerols, and free fatty acids [37], but it is unlikely that the amounts excreted by the parasite make a substantial contribution to the host metabolic profile, given that the total parasite mass to host weight ration is ∼1:300.…”

Section: Discussionsupporting

confidence: 91%

Metabolic Profiling of an Echinostoma caproni Infection in the Mouse for Biomarker Discovery

Saric

Wang

et al. 2008

PLoS Negl Trop Dis

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BackgroundMetabolic profiling holds promise with regard to deepening our understanding of infection biology and disease states. The objectives of our study were to assess the global metabolic responses to an Echinostoma caproni infection in the mouse, and to compare the biomarkers extracted from different biofluids (plasma, stool, and urine) in terms of characterizing acute and chronic stages of this intestinal fluke infection.Methodology/Principal FindingsTwelve female NMRI mice were infected with 30 E. caproni metacercariae each. Plasma, stool, and urine samples were collected at 7 time points up to day 33 post-infection. Samples were also obtained from non-infected control mice at the same time points and measured using 1H nuclear magnetic resonance (NMR) spectroscopy. Spectral data were subjected to multivariate statistical analyses. In plasma and urine, an altered metabolic profile was already evident 1 day post-infection, characterized by reduced levels of plasma choline, acetate, formate, and lactate, coupled with increased levels of plasma glucose, and relatively lower concentrations of urinary creatine. The main changes in the urine metabolic profile started at day 8 post-infection, characterized by increased relative concentrations of trimethylamine and phenylacetylglycine and lower levels of 2-ketoisocaproate and showed differentiation over the course of the infection.Conclusion/SignificanceThe current investigation is part of a broader NMR-based metabonomics profiling strategy and confirms the utility of this approach for biomarker discovery. In the case of E. caproni, a diagnosis based on all three biofluids would deliver the most comprehensive fingerprint of an infection. For practical purposes, however, future diagnosis might aim at a single biofluid, in which case urine would be chosen for further investigation, based on quantity of biomarkers, ease of sampling, and the degree of differentiation from the non-infected control group.

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Section: Discussionsupporting

confidence: 91%

Metabolic Profiling of an Echinostoma caproni Infection in the Mouse for Biomarker Discovery

Saric

Wang

et al. 2008

PLoS Negl Trop Dis

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“…(Muller et al 1999) ; E. trivolvis redial bodies ) ; E. caproni adults and excysted metacercariae (Schneck et al 2004a) ; and SCW and feces from Biomphalaria glabrata infected with E. caproni (Schneck et al 2004b) . The Folch procedure involves extraction of the sample with chloroformmethanol (2:1) in a glass homogenizer followed by washing the organic phase with 0.88% KCl.…”

Section: Sample Preparationmentioning

confidence: 97%

“…The concentrations of FS were determined in all stages by densitometry. Schneck et al (2004a) used HPTLC to analyze neutral lipids in worm incubates isotonic, hypotonic, and hypertonic to the mouse intestinal habitat of E. caproni adults. An array of neutral lipids was found in all incubate samples, the most abundant of which was TG.…”

Section: Lipids In Echinostomesmentioning

confidence: 99%

Studies of echinostomes using chromatography and atomic spectrometry.

Sherma

Fried

2008

The Biology of Echinostomes

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“…The concentrations of free sterols were determined in all stages by densitometry. Schneck et al (2004a) used HPTLC to analyze the neutral lipids in the incubates from E. caproni excysted metacercariae. Several neutral lipids were found in the incubates, the most abundant of which was the triacylglycerol fraction.…”

Section: Thin-layer Chromatography and Larval Echinostomesmentioning

confidence: 99%

The use of echinostomes to study host–parasite relationships between larval trematodes and invertebrate and cold-blooded vertebrate hosts

2007

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Echinostomes are intestinal trematodes with life cycles that are easy and inexpensive to maintain in the laboratory. For this reason, echinostomes have served for years as experimental models in different areas of parasitology. However, the usefulness of these trematodes in experimental parasitology has been under estimated. In this paper, we discuss the life cycles of echinostomes and the techniques used to maintain them in the laboratory. We further examine the characteristics of these trematodes that make them useful models for the analysis of larval parasite-host relationships. Echinostomes are useful for studies on the immunobiology of snails, host-finding processes of free-living larval stages, effects of larval trematode infections on anuran populations, and studies on analytes in the larval trematodes and their snail intermediate hosts.

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Effects of tonicity on the release of neutral lipids in Echinostoma caproni adults and observations on lipids in excysted metacercariae

Cited by 7 publications

References 15 publications

Metabolic Profiling of an Echinostoma caproni Infection in the Mouse for Biomarker Discovery

Metabolic Profiling of an Echinostoma caproni Infection in the Mouse for Biomarker Discovery

Studies of echinostomes using chromatography and atomic spectrometry.

The use of echinostomes to study host–parasite relationships between larval trematodes and invertebrate and cold-blooded vertebrate hosts

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