Effects of Dietary Addition of Perilla frutescens Seeds on the Content of Polyunsaturated Fatty Acids in Egg Yolk of Gallus domesticus

Abstract

This study aims to evaluate the co-expression of specific AT sequence binding protein 2 (SATB2 Group) and Cytokeratin 20 (CK20 Group) in mucinous ovarian tumors and their correlation with tumor pathological classification and prognostic outcome. One hundred and sixty cases of ovarian mucinous tumors diagnosed from January 2018 and January 2020 were selected. They were divided into four groups: mucinous cystadenocarcinoma group (age 62.78±7.92, n=53), borderline mucinous cystadenocarcinoma group (age 63.82±9.26, n=55), mucinous cystadenocarcinoma group (age 62.81±6.28, n=52) Group and normal ovarian group (age 62.71±7.97, n=160). All cases were examined by two gynecological pathologists. The expression levels of SATB2 and CK20 in different groups were detected by immunohistochemical staining. SATB2 in mucinous ovarian tumors had a diagnostic sensitivity of 82%, a specificity of 78%, and an overall accuracy rate of 82%. CK20 in mucinous ovarian tumors had a diagnostic sensitivity of 94%, a specificity of 59%, and an overall accuracy rate of 85%. SATB2/CK20 in mucinous ovarian tumors has a diagnostic sensitivity of 65%, a specificity of 99%, and an overall accuracy rate of 89%. Compared with SATB2/CK20 single staining, SATB2/CK20 double staining had lower sensitivity, higher specificity and increased overall accuracy. The expression levels of SATB2 and CK20 were not related to the patient's age, menstrual status and tumor diameter (P>0.05), but was related to recurrence, pathological type, tissue differentiation, prognostic outcome, disease course and clinical stage (P<0.05). According to immunohistochemical examination, the co-expression of SATB2 and CK20 could improve the specificity of mucinous ovarian tumors. In addition, SATB2/CK20 co-expression is related to recurrence, pathological type, tissue differentiation, prognostic outcome, disease course and clinical stage.

Effects of Perilla Seed Meal on Productive Performance, Egg Quality, Antioxidant Capacity and Hepatic Lipid Metabolism of Wenchang Breeder Hens

“…Previous studies have shown that PS supplementation in the diet of laying hens significantly increases the levels of PUFAs in their eggs [26,27]. Consistent with previous research, dietary PSM had a significant impact on the fatty acid composition of the egg yolks.…”

“…Previous studies have found that perilla seed extract (PSE) lowers blood lipids in mice and breeder hens and affects egg quality and fatty acid composition [23][24][25]. PS consumption by the hens could also influence the unsaturated fatty acid content of the eggs and thus improve their nutrition [26,27]. Since PSM is rich in high-quality fats and nutrients, these experiments examined whether PSM can affect liver lipid metabolism and antioxidant capacity in breeder hens, as well as the effects of PSM on laying performance, egg quality and fatty acid content of the eggs.…”

A Novel Strategy for the Production of Edible Insects: Effect of Dietary Perilla Seed Supplementation on Nutritional Composition, Growth Performance, Lipid Metabolism, and Δ6 Desaturase Gene Expression of Sago Palm Weevil (Rhynchophorus ferrugineus) Larvae

“…PS formulation led to a significant increase in C18:3 (all-cis-cis-9,12,15-linolenic acid; ALA), which was 8–26 times greater than the control diet ( p < 0.05). This led to an increase in n-3 FA enhancement following PS fortification into PI mixed GSPT diets, resulting in PS being an excellent source of ALA [ 19 ]. SFA, MUFA, and PUFA differed statistically between the test diets ( p < 0.05), ranging from 16.87 to 47.26 g/100 g total lipid, 13.90 to 36.55 g/100 g lipid, and 8.96 to 66.94 g/100 g lipid, respectively ( Table 1 ).…”

“…However, higher PS intake in SPWL fed on high PS-formulated diets may alter mineral absorption from the gut by chelating with phytate, resulting in lower element uptake. The Zn content of these SPWL was higher than that of Gryllus assimilis (52.2 mg/kg) and Zophobas morio (24.7 mg/kg), with both insects having higher Fe content (27.8 and 22.7 mg/kg, respectively) than the SPWL [ 19 ]. Infants, children, teenagers, and women of childbearing age, particularly pregnant women, are the most vulnerable to iron deficiency [ 33 ].…”

“…By significantly altering the FA composition of SPWL, it is possible to obtain healthier lipids for hypercholesterolemic or diabetic people by feeding SPWL with 7-20% PS-formulated diets. 0.22 ± 0.07a 0.10 ± 0.03a 0.13 ± 0.13a 0.06 ± 0.06a 0.07 ± 0.08a 0.04 ± 0.02a 0.01 ± 0.01a C18:3 (all-cis-cis -9,12, 15) 0.08 ± 0.01e 0.17 ± 0.02e 0.83 ± 0.07d 0.99 ± 0.05d 1.90 ± 0.05c 4.97 ± 0.06b 8.40 ± 0.45a C20:0 0.09 ± 0.05a 0.08 ± 0.04a 0.07 ± 0.04a 0.08 ± 0.02a 0.09 ± 0.02a 0.06 ± 0.01a 0.09 ± 0.00a C20:3 (all-cis-8,11,14) 0.22 ± 0.05a 0.03 ± 0.02b 0.12 ± 0.13ab 0.03 ± 0.04b 0.06 ± 0.04b 0.04 ± 0.03b 0.04 ± 0.01b C20:3 (all-cis-11,14,17) 0.07 ± 0.03a 0.04 ± 0.02a 0.06 ± 0.02a 0.06 ± 0.02a 0.06 ± 0.05a 0.05 ± 0.04a 0.03 ± 0.03a C20:4n6 0.06 ± 0.01abc 0.06 ± 0.02ab 0.04 ± 0.02bc 0.04 ± 0.01c 0.07 ± 0.00a 0.01 ± 0.00d 0.01 ± 0.01d C20:5 (all -cis-5,8,11,14,17) 0.03 ± 0.02b 0.02 ± 0.01b 0.04 ± 0.00b 0.02 ± 0.01b 0.06 ± 0.00a 0.03 ± 0.00b 0.02 ± 0.01b C22:2 (all-cis-13,16) 0.04 ± 0.02a 0.06 ± 0.04a 0.06 ± 0.06a 0.03 ± 0.01a 0.05 ± 0.05a 0.04 ± 0.04a 0.04 ± 0.01a C22:6 (all-cis-4,7,10, 13,16,19) 0.04 ± 0.01b 0.05 ± 0.01b 0.08 ± 0.02a 0.06 ± 0.00b 0.05 ± 0.02b 0.03 ± 0.00b 0.04 ± 0.00b Other 2.17 ± 0.60a 1.39 ± 0.33a…”