This study aims to evaluate the co-expression of specific AT sequence binding protein 2 (SATB2 Group) and Cytokeratin 20 (CK20 Group) in mucinous ovarian tumors and their correlation with tumor pathological classification and prognostic outcome. One hundred and sixty cases of ovarian mucinous tumors diagnosed from January 2018 and January 2020 were selected. They were divided into four groups: mucinous cystadenocarcinoma group (age 62.78±7.92, n=53), borderline mucinous cystadenocarcinoma group (age 63.82±9.26, n=55), mucinous cystadenocarcinoma group (age 62.81±6.28, n=52) Group and normal ovarian group (age 62.71±7.97, n=160). All cases were examined by two gynecological pathologists. The expression levels of SATB2 and CK20 in different groups were detected by immunohistochemical staining. SATB2 in mucinous ovarian tumors had a diagnostic sensitivity of 82%, a specificity of 78%, and an overall accuracy rate of 82%. CK20 in mucinous ovarian tumors had a diagnostic sensitivity of 94%, a specificity of 59%, and an overall accuracy rate of 85%. SATB2/CK20 in mucinous ovarian tumors has a diagnostic sensitivity of 65%, a specificity of 99%, and an overall accuracy rate of 89%. Compared with SATB2/CK20 single staining, SATB2/CK20 double staining had lower sensitivity, higher specificity and increased overall accuracy. The expression levels of SATB2 and CK20 were not related to the patient's age, menstrual status and tumor diameter (P>0.05), but was related to recurrence, pathological type, tissue differentiation, prognostic outcome, disease course and clinical stage (P<0.05). According to immunohistochemical examination, the co-expression of SATB2 and CK20 could improve the specificity of mucinous ovarian tumors. In addition, SATB2/CK20 co-expression is related to recurrence, pathological type, tissue differentiation, prognostic outcome, disease course and clinical stage.

In order to study the effect of dietary supplement of linseed oil on polyunsaturated fatty acids (PUFAs) in egg yolk of Gallus domestiaus, total 160 healthy Gallus domestiaus of 26-week old were randomly selected and divided into 4 groups, each of which included 40 chickens fed outdoors with an area of 63 m2 and free food and drinking water. The control group (CK) was fed the basic diet, and the experimental group was added 1, 3 and 5% of linseed oil in the fundamentals of diet (experimental group 1, 2 and 3, abbreviated as P1, P2, P3), respectively. The content of PUFA and the value of n-6 PUFA and n-3 PUFA in egg yolk were determined by gas chromatography with 24 eggs randomly selected in each group after 10th, 25th and 40th days of the commence of the experiment. We found that the levels of α-linolenic acid (C18:3n3, ALA) and docosahexaenoic acid (C22:6n3, DHA) in egg yolk were clearly higher than the CK when 3% linseed oil or 5% linseed oil were added in the diet during the three experiment periods, in which the contents of linoleic Acid (18:2n6c, LA) and arachidonic acid (C20:4n6, ARA) in egg yolk differ little between P1, P2 and P3 and the CK while the value of n-6 PUFA and n-3 PUFA in egg yolk of the P2 and P3 groups decreased significantly. The comprehensive comparisons suggested that the content of C18:3n3 and C22:6n3 in egg yolk could be clearly increased in the P2 while the value of n-6 PUFA and n-3 PUFA decreased. This study provides theoretical basis for the production of functional eggs enriched with C22:6n3 and C18:3n3.

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The safe and reliable operation of a distribution network (DN) is closely related to the development of national economy and the improvement of people's living standards. At present, the design principle of a distribution network is still based on a primary system and primary equipment, without considering the relay protection on the secondary side, which is an important guarantee for the safe and reliable operation of a power grid. The authors theoretically proved the influence of network structures on the main protection (instantaneous overcurrent protection and time-delay instantaneous overcurrent protection) of conventional stage-type current protection, and main influencing factors are analysed and verified in an example. The result shows that reasonable planning and design are an important premise for overcurrent protection to meet sensitivity requirements, and it shows a theoretical and practical guiding significance for the planning of a distribution network. INTRODUCTIONElectricity consumers are directly connected to a distribution network (DN), where a failure rate is high, so the quality of the construction and reformation of a DN directly affects the development of national economy and the improvement of people's material and cultural living standards. DN refers to the network directly connected with users in the power system. Document [1-3] only improves the primary side of the system in the construction of DN, and relay protection, as the secondary side, is relatively weak in the early stage of design. In document [4], it is proposed that the development of relay protection technology should adhere to four performance principles: reliability, rapidity, selectivity and sensitivity. At the same time, it is pointed out that we should abide by this principle in all links of design, manufacturing, construction and operation from the perspective of power grid. Literature [5,6] only considers the influence of reliability unilaterally, and makes decisions on variables such as new lines and upgraded lines of distribution network by analysing the influencing factors. Document [7] designs a protection scheme, which can quickly and accurately judge the fault line, and can improve the selectivity and rapidity of the traditional phased current protection when applied to different scenarios.

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