Effects of dietary antioxidant supplementation on oxidative damage and resistance to oxidative damage during prolonged exercise in sled dogs

Baskin, Carole R.; Hinchcliff, Kenneth W.; DiSilvestro, Robert A.; Reinhart, Gregg A.; Hayek, Michael G.; Chew, Boon P.; Burr, John; Swenson, Richard A.

doi:10.2460/ajvr.2000.61.886

Cited by 64 publications

(43 citation statements)

References 27 publications

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“…Another study has demonstrated that fish oil decreased MDA levels in healthy dogs [68]. The slight elevation in values of 8-OH-dG that we found in both fish and corn oil groups was non-significant and fell below the reported plasma values for healthy dogs [69]. It indicates that during the study period the dogs did not exhibit evidence of DNA damage detected by measuring 8-OH-dG either before or after receiving fish or corn oil.…”

Section: Discussionmentioning

confidence: 52%

Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

et al. 2016

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BackgroundOxidative stress plays an important role in the pathogenesis of disease, and the antioxidant physiological effect of omega-3 from fish oil may lead to improvement of canine spontaneous osteoarthritis (OA).MethodsIn this prospective randomized, controlled, double-blinded study, we assessed haematological and biochemical parameters in dogs with OA following supplementation with either a concentrated omega-3 deep sea fish oil product or corn oil. Blood samples from 77 client-owned dogs diagnosed as having OA were taken before (baseline) and 16 weeks after having orally ingested 0.2 ml/Kg bodyweight/day of deep sea fish oil or corn oil. Circulating malondialdehyde (MDA), glutathione (GSH), non-transferrin bound iron (NTBI), free carnitine (Free-Car), 8-hydroxy-2-deoxyguanosine (8-OH-dG), and serum fatty acids, haemograms and serum biochemistry were evaluated. Differences within and between groups from baseline to end, were analysed using repeated samples T-test or Wilcoxon rank test and independent samples T-test or a Mann-Whitney test.ResultsSupplementation with fish oil resulted in a significant reduction from day 0 to day 112 in MDA (from 3.41 ± 1.34 to 2.43 ± 0.92 μmol/L; P < 0.001) and an elevation in Free-Car (from 18.18 ± 9.78 to 21.19 ± 9.58 μmol/L; P = 0.004) concentrations, whereas dogs receiving corn oil presented a reduction in MDA (from 3.41 ± 1.34 to 2.41 ± 1.01 μmol/L; P = 0.001) and NTBI (from −1.25 ± 2.17 to −2.31 ± 1.64 μmol/L; P = 0.002). Both groups showed increased (albeit not significantly) GSH and 8-OH-dG blood values. Dogs supplemented with fish oil had a significant reduction in the proportions of monocytes (from 3.84 ± 2.50 to 1.77 ± 1.92 %; P = 0.030) and basophils (from 1.47 ± 1.22 to 0.62 ± 0.62 %; P = 0.012), whereas a significant reduction in platelets counts (from 316.13 ± 93.83 to 288.41 ± 101.68 × 109/L; P = 0.029), and an elevation in glucose (from 5.18 ± 0.37 to 5.32 ± 0.47 mmol/L; P = 0.041) and cholesterol (from 7.13 ± 1.62 to 7.73 ± 2.03 mmol/L; P = 0.011) measurements were observed in dogs receiving corn oil.ConclusionsIn canine OA, supplementation with deep sea fish oil improved diverse markers of oxidative status in the dogs studied. As corn oil also contributed to the reduction in certain oxidative markers, albeit to a lesser degree, there was no clear difference between the two oil groups. No clinical, haematological or biochemical evidence of side effects emerged related to supplementation of either oil. Although a shift in blood fatty acid values was apparent due to the type of nutraceutical product given to the dogs, corn oil seems not to be a good placebo.

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Section: Discussionmentioning

confidence: 52%

Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

et al. 2016

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“…Associated supplementation with vitamin E, beta-carotene and lutein has been shown to decrease 8-OHdG levels after 3 days of endurance training in sled dogs (Baskin et al 2000). Prevention of DNA damage by vitamin E after an incremental exercise test to exhaustion has also been demonstrated (Hartmann et al 1995).…”

Section: Discussionmentioning

confidence: 97%

The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists

et al. 2005

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The objective of this study was to test the effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress in moderately trained cyclists. A double-blind study was conducted in moderately trained cyclists. They were randomly allocated to receive either an antioxidant (13 subjects) or a placebo (13 subjects) 15 min pre-exercise (30 ml.kg(-1)) and during a 90 min constant-load test (30 ml.kg(-1).15 min(-1)) on a bicycle ergometer at 70% VO2max The supplemented beverage contained black grape (81 g/l), raspberry (93 g/l) and red currant (39 g/l) concentrates, and its total antioxidant capacity, measured by the ABTS(.+) technique, was 0.41 mM Trolox. No significant difference from basal to post-exercise period was detected for plasma TBARS in either the placebo group or the group receiving the antioxidant supplemented beverage. Post-exercise carbonyls decreased by 29% in the group receiving antioxidants, and the pattern of change was significantly different between antioxidant and placebo conditions. The urinary excretion of 8-OHdG increased significantly by 21% in the placebo group. Again differences in the pre- to post-exercise change were significant between both conditions These results suggest that in moderately trained cyclists, antioxidant supplementation counters oxidative stress induced by a 90 min exercise at 70% VO2max.

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“…Currently, field studies of dogs performing specific activities and reported in the veterinary literature include investigations of sled dogs (Hinchcliff et al, 1993(Hinchcliff et al, , 1997Querengaesser et al, 1994;Burr et al, 1997;Baskin et al, 2000;Piercy et al, 2000Piercy et al, , 2001aPiercy et al, , 2001bDunlap et al, 2006;McKenzie et al, 2007;Angle et al, 2009;Wakshlag et al, 2010) and Greyhounds (Lassen et al, 1986;Snow et al, 1988;Ilkiw et al, 1989;Rose and Bloomberg, 1989;Nold et al, 1991;Pieschl et al, 1992). These two groups represent large, accessible populations of canine athletes that are often relatively uniform in age and genetic background, and the studies demonstrate that important changes do occur in the exercising canine.…”

Section: Introductionmentioning

confidence: 98%

Serum biochemical, blood gas and antioxidant status in search and rescue dogs before and after simulated fieldwork

Spoo¹,

Zoran

Downey³

et al. 2015

The Veterinary Journal

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Effects of dietary antioxidant supplementation on oxidative damage and resistance to oxidative damage during prolonged exercise in sled dogs

Cited by 64 publications

References 27 publications

Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

Evaluating oxidative stress, serological- and haematological status of dogs suffering from osteoarthritis, after supplementing their diet with fish or corn oil

The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists

Serum biochemical, blood gas and antioxidant status in search and rescue dogs before and after simulated fieldwork

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