Effects of Dietary Barley Starch Contents on the Performance, Nutrient Digestion, Rumen Fermentation, and Bacterial Community of Fattening Hu Sheep

Ma, Xiaowen; Zhou, Wenjing; Guo, Tongqing; Li, Fēi; Li, Fadi; Tao, Ran; Zhang, Zhian; Guo, Long

doi:10.3389/fnut.2021.797801

Cited by 6 publications

(6 citation statements)

References 43 publications

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“…These results indicated that a high barley starch level in the diet promoted the proliferation of amylolytic bacteria, but the abundances of pH-sensitive bacteria decreased. Compared with the previous study by Ma et al [ 8 ], more differences in the microflora of the rumen were observed in this trial. The reason for the differences between these studies could be due to the different animals, with fattening sheep of a similar age (about 3 months) and initial BW (29.70 ± 1.70 kg) used in the former, while fistula sheep of about 10 months age and an initial BW of 53.78 ± 3.14 kg were used in our study.…”

Section: Discussioncontrasting

confidence: 92%

“…However, barley has a higher amylopectin content than corn, which can be fermented and digested in the rumen to a greater extent than corn and could be a potential replacement for corn as a source of energy for ruminants [ 5 , 6 ]. Combining barley starch (BS) with corn starch (CS) could improve the efficiency of starch digestion by balancing starch fermentation in the rumen or small intestine [ 3 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, most studies usually involve one source of non-structural carbohydrates being completely replaced by another source of carbohydrates, but research data about the effect of different ratios of two non-structural carbohydrates in the diet of sheep on rumen fermentation and microflora are relatively limited [ 11 , 12 ]. Our previous study has shown that replacing CS with BS in the diet affected the performance, nutrient digestion and rumen fermentation of Hu sheep [ 8 ]; one main reason for this might be due to changes in the rumen microflora. However, insufficient attention was paid to the effects on rumen microflora in the previous study; a few bacteria were determined, which could not reflect an alteration of the rumen’s microbial structure.…”

Section: Introductionmentioning

confidence: 99%

“…However, insufficient attention was paid to the effects on rumen microflora in the previous study; a few bacteria were determined, which could not reflect an alteration of the rumen’s microbial structure. In addition, Ma et al also found that, within a day of equipping sheep with fistulas, the mean pH decreased with increasing dietary proportions of BS, which would change microbial structure in the rumen [ 8 ]; unfortunately, there was no relevant information to confirm this. Further research is needed to clarify the effects of replacing corn starch with barley starch on the rumen’s microbial diversity and richness, and on the bacteria that dominate at the phylum or genus level.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Barley Starch Level in Diet on Fermentation and Microflora in Rumen of Hu Sheep

Zhang

et al. 2022

Animals

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This study aimed to explore the effects of different levels of barley starch instead of corn starch on the rumen fermentation and microflora when feeding a corn-based diet to Hu sheep. Thirty-two male Hu sheep equipped with permanent rumen fistulas were selected and fed in individual metabolic cages. All sheep were randomly divided into four groups (eight sheep in each group) and fed with four diets containing a similar starch content, but from different starch sources, including 100% of starch derived from corn (CS), 33% of starch derived from barley + 67% of starch derived from corn (33 BS), 67% of starch derived from barley + 33% of starch derived from corn (67 BS) and 100% of starch derived from barley (100 BS). The experimental period included a 14 d adaptation period and a 2 d continuous data collection period. The results showed that the molar proportions of acetate, isobutyrate, butyrate and isovalerate and the ratio of acetate to propionate in the 67 BS and 100 BS groups decreased compared with the CS and 33 BS groups (p < 0.001), while the molar proportions of propionate and valerate increased (p < 0.001). The combination of 33% barley starch and 67% corn starch in the diet improved the production of TVFAs (p = 0.007). The OTUs and Shannon indexes of the CS and 33 BS groups were higher than the 67 BS and 100 BS groups (p < 0.001), and the Chao1 and Ace indexes were higher than the 100 BS group (p < 0.05). In addition, the 33 BS group had increased the relative abundances of Bacteroidetes, Prevotella and Ruminococcus and the abundances of Fibrobacter succinogenes, Ruminococcus flavefaciens, Streptococcus bovis, Selenomonas ruminantium and Prevotella brevis relative to the CS group (p < 0.05). These results indicate that the substitution of 33% of the CS with BS did not change the rumen fermentation pattern relative to the CS group, and increased the richness and diversity of the rumen microbes in Hu sheep compared with other two starch substitute groups.

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Section: Discussioncontrasting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Barley Starch Level in Diet on Fermentation and Microflora in Rumen of Hu Sheep

Zhang

et al. 2022

Animals

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“…A high-energy dietary formulation alters the rumen micro-ecosystem accordingly, leading to improvements in performance traits [ 10 ]. Feeding fattening Hu sheep a high-concentrate corn-based diet improves the performance and rumen fermentation indexes [ 11 ]. There are sophisticated exchanges between ruminants and rumen microorganisms.…”

Section: Introductionmentioning

confidence: 99%

High-energy diet modify rumen microbial composition and microbial energy metabolism pattern in fattening sheep

Yang

et al. 2023

BMC Vet Res

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Higher dietary energy is often used to achieve better animal performance in mutton sheep production. Notably, changing the diet formula affects rumen fermentation and the microbiota of ruminants. In this study, we investigated the effect of dietary energy on rumen fermentation and ruminal microbiota in fattening sheep. Fifteen 2-month-old white-headed Suffolk sheep (♂) × Hu sheep (♀) crossbred lambs were randomly divided into three treatments based on the dietary energy of the feeds fed: 8.67 MJ/kg (Low energy (LE); n = 5), 10.38 MJ/kg (standard energy (CON); n = 5), and 12.31 MJ/kg (high energy (HE); n = 5) groups. After 70 days of feeding, sheep were slaughtered and the ruminal fluids were collected and analyzed to determine fermentation parameters. Microbiota was determined using metagenomics sequencing. Notably, the microbial cell protein (MCP) and butyric acid concentrations were significantly high in the HE group. Metagenomic sequencing revealed that ACE and Chao indexes of the HE group were significantly decreased. Four genera among the major classified taxa across all the kingdoms differed in relative abundance in the three dietary energy levels. The relative abundances of Prevotella_brevis, Succiniclasticum_ruminis, Prevotellace-ae_bacterium, and Lachnospiraceae_bacterium were significantly correlated with rumen fermentation. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis further revealed that a high-energy diet increased lipid metabolism of microbiota. The Carbohydrate Active enzymes (CAZy) gene, which participates in energy metabolism, was upregulated, while genes regulating plant cell wall degradation were downregulated in the HE group. These results suggest that a high-energy diet had minimal influence on the rumen fermentation pattern but altered the composition of the rumen microbiota, enhancing microbial lipid metabolism and limiting crude fiber metabolism. The findings of this study provide scientific evidence of the effect of dietary energy on ruminant fermentation and fattening sheep production.

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Variability in chewing, ruminal fermentation, digestibility and bacterial communities between subacute ruminal acidosis-susceptible and acidosis-tolerant sheep

Zhang

et al. 2023

animal

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Effects of Dietary Barley Starch Contents on the Performance, Nutrient Digestion, Rumen Fermentation, and Bacterial Community of Fattening Hu Sheep

Cited by 6 publications

References 43 publications

Effects of Barley Starch Level in Diet on Fermentation and Microflora in Rumen of Hu Sheep

Effects of Barley Starch Level in Diet on Fermentation and Microflora in Rumen of Hu Sheep

High-energy diet modify rumen microbial composition and microbial energy metabolism pattern in fattening sheep

Variability in chewing, ruminal fermentation, digestibility and bacterial communities between subacute ruminal acidosis-susceptible and acidosis-tolerant sheep

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