Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes

Wang, Qiye; Wang, Yancan; Wang, Xin; Dai, Chunpeng; Tang, Wensheng; Li, Jianzhong; Huang, Pengfei; Li, Yali; Ding, Xueqin; Huang, Jing; Hussain, Tarique; Yang, Huansheng; Zhu, Mingzhi

doi:10.1002/fsn3.1955

Cited by 20 publications

(26 citation statements)

References 59 publications

(70 reference statements)

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“…The concentrations of total VFA (TVFA) and individual VFA were detected by gas chromatography (Agilent 7890A, NYSE: A, Palo Alto, CL, United States), referring to the method of Wang et al (2020b). A total of 0.15 ml of 25% metaphosphoric acid was added into 1.5 ml of rumen fluid samples and was properly mixed.…”

Section: Rumen Fermentation Parametersmentioning

confidence: 99%

“…Hematoxylin-eosin (HE) staining and optical microscopy were used for the rumen and intestinal morphology analysis. According to the method described by Wang et al (2020b) and Deng et al (2020), paraffin sections of tissues were fixed.…”

Section: Rumen and Intestinal Morphologymentioning

confidence: 99%

“…The Hu sheep is a unique breed native to China, and is one of the several white lamb-skin sheep breeds in the world (Wang et al, 2020a). Commonly, Hu sheep are identified as an ideal choice for factory production in our country, especially in southern agricultural areas (Wang et al, 2020b). With the deterioration of ecological environments and the increasing demand for consumption of lamb, ruminant production with traditional grazing patterns is dominated instead by large-scale farming with stall-feeding.…”

Section: Introductionmentioning

confidence: 99%

“…Bacteria are the dominant microorganism group (Pitta et al, 2016), and some of these microorganisms that are attached to feed particles participate in the transformation of plant ingredients into animal products (Preston and Leng, 1987;Han et al, 2015). Rumen bacterial diversity is influenced by diet, breed, host age, season, and geographic region (Lee et al, 2012), and diet is a key factor in determining its composition and function (Wang et al, 2020b). The energy and protein of dietary nutrition are the two determinants that most often limit microbial activity (Clark, 1975;Clark and Davis, 1980).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Dietary Energy Levels on Rumen Fermentation, Gastrointestinal Tract Histology, and Bacterial Community Diversity in Fattening Male Hu Lambs

Wang¹,

Zeng²,

Zeng³

et al. 2021

Front. Microbiol.

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This study investigated rumen fermentation and histological and microbial diversity in male Hu lamb fed diets with different metabolizable energy (ME) levels (MEA, 9.17 MJ/kg, MEB, 10.00 MJ/kg, and MEC, 10.82 MJ/kg). Thirty-six male Hu lambs were randomly allotted to three treatments, and the feeding trial lasted for 67 days. Rumen fermentation results suggest that the iso-valerate had a significant effect on dietary energy level. The papillary height (PH) of rumen was the highest in the MEB group, the crypt depth (CD) was significantly increased in the duodenum and jejunum, and the villus height (VH)-to-CD ratio (VH/CD) was significantly decreased in the duodenum by increasing dietary energy levels; the VH, villus width (VW), and VH/CD also had significant differences in the ileum. 16S rRNA sequencing results showed that the operational taxonomic units (OTUs) number, the ACE, and Chao1 indices were linearly decreased by increasing dietary energy level; 24 phyla including 124 genera were identified, and the relative abundance of Papillibacter and Quinella linearly decreased by increasing the dietary energy level. Compared to MEA and MEB groups, the relative abundance of unidentified_Veillonellaceae and Anaerovibrio was significantly increased in the MEC group at the genus level. The relative abundance of the carbohydrate metabolism pathway predicted by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was linearly increased by increasing the dietary energy levels. Three metabolic pathways identified in Kyoto Encyclopedia of Genes and Genomes (KEGG) level 3 were significantly influenced as the dietary energy level increased. In summary, these results demonstrated that the dietary energy levels affected the rumen fermentation parameters, morphological structures of the gastrointestinal tract (GIT), and the composition and function of rumen microflora in male Hu sheep.

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Section: Rumen Fermentation Parametersmentioning

confidence: 99%

Section: Rumen and Intestinal Morphologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Dietary Energy Levels on Rumen Fermentation, Gastrointestinal Tract Histology, and Bacterial Community Diversity in Fattening Male Hu Lambs

Wang¹,

Zeng²,

Zeng³

et al. 2021

Front. Microbiol.

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“…The microbiota’s F:B ratio was reversed between the rumen and the duodenum due to differences in the organs and functions. The most potent organ, which degrades and converts plant materials to SCFAs in the ruminants, is the rumen ( Wang et al 2020 ). It possesses the complex microbiota that plays a vital role in the fermentation of feed and energy metabolism, and the SCFAs provide more than 70% of the energy to guarantee host growth and reproduction performance ( Flint et al 2007 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of the Microbiota Between Rumen and Duodenum of Twin Lambs Based on Diets of Ceratoides or Alfalfa

Akonyani

Feng

Ying

et al. 2021

Polish Journal of Microbiology

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In our previous study, diet directly impacted the microbiota of the rumen in twin lambs. The duodenum is the first part of the small intestine, so we seek to determine whether there is a difference in the digesta between the two feed groups HFLP (high fiber, low protein) and LFHP (low fiber, high protein), and its impact on the biodiversity and metabolism of the duodenum. Results showed that the number of Operational Taxonomic Units (OTUs) in the duodenum (2,373 OTUs) was more than those in the rumen (1,230 OTUs), and 143 OTUs were significantly different in the duodenum between the two groups. The two most predominant phyla were Bacteriodetes and Firmicutes, but this ratio was reversed between the rumen and duodenum of lambs fed different feedstuffs. The difference in the digesta that greatly changed the biodiversity of the rumen and duodenum could affect the microbial community in the gastrointestinal tract (GIT). Sixteen metabolites were significantly different in the duodenum between the two groups based on the metabolome analysis. The relationships were built between the microbiome and the metabolome based on the correlation analysis. Some metabolites have a potential role in influencing meat quality, which indicated that the diet could affect the microbiota community and finally change meat quality. This study could explain how the diet affects the rumen and duodenum’s microbiota, lay a theoretical basis for controlling feed intake, and determine the relationship between the duodenum’s microbiota and metabolism.

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Oleuropein Prevents OVA‐Induced Food Allergy in Mice by Enhancing the Intestinal Epithelial Barrier and Remodeling the Intestinal Flora

Guo

et al. 2022

Molecular Nutrition Food Res

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Scope This study assesses whether oleuropein prevents ovalbumin (OVA)‐induced food allergy (FA) and investigates the underlying mechanisms. Methods and results A Balb/c FA mouse model is established and maintained for 7 weeks. The subjects are administered OVA by oral gavage to induce FA and supplemented with different oleuropein doses (1.00–20.00 mg kg−1 per day) to evaluate its preventative efficacy. The results indicate that oleuropein effectively alleviates OVA‐induced allergy symptoms and promotes temperature elevation in sensitized mice. The secretion of serology‐specific OVA‐immunoglobulin (Ig)E, OVA‐IgG, and histamine is inhibited in the sensitized mice. Oleuropein dramatically upregulates the expression of intestinal tight junction (TJ) proteins, regenerating gene (Reg) IIIγ, and interleukin (IL)‐22, enhancing the physical and biochemical barrier function of the intestinal epithelium. Additionally, oleuropein improves the immune homeostasis of the intestinal epithelium by affecting the function of mucosal mast cells and regulatory T (Treg) cells. The disordered intestinal flora of the sensitized mice also improves after oleuropein administration. Conclusions These findings suggest that oleuropein prevents FA by enhancing intestinal epithelial barrier function and improving immune homeostasis and intestinal flora in sensitized mice. Therefore, diets rich in oleuropein should be recommended for people with FA.

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Effects of dietary energy levels on rumen fermentation, microbiota, and gastrointestinal morphology in growing ewes

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 20 publications

References 59 publications

Effects of Dietary Energy Levels on Rumen Fermentation, Gastrointestinal Tract Histology, and Bacterial Community Diversity in Fattening Male Hu Lambs

Effects of Dietary Energy Levels on Rumen Fermentation, Gastrointestinal Tract Histology, and Bacterial Community Diversity in Fattening Male Hu Lambs

Comparative Analysis of the Microbiota Between Rumen and Duodenum of Twin Lambs Based on Diets of Ceratoides or Alfalfa

Oleuropein Prevents OVA‐Induced Food Allergy in Mice by Enhancing the Intestinal Epithelial Barrier and Remodeling the Intestinal Flora

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