Effects of dietary forage to concentrate ratio on nutrient digestibility, ruminal fermentation and rumen bacterial composition in Angus cows

Chen, Hao; Wang, Chunjie; Simujide, Huasai; Chen, Aorigele

doi:10.1038/s41598-021-96580-5

Cited by 58 publications

(38 citation statements)

References 67 publications

(86 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increasing the concentrate proportion increased ( p < 0.05) DM intake and digestibility of DM, OM, and CP but decreased ( p < 0.05) NDF and ADF digestibility. These findings agree with Chen et al [ 27 ], cow fed a high concentrate diet showed significantly increased digestibility of nutrients. It could be due to the concentrate diet can provide additional nutrients for rumen microbial growth and can enhance rumen fermentation.…”

Section: Resultssupporting

confidence: 93%

The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle

Phesatcha

Wanapat

Cherdthong

2021

Animals

View full text Add to dashboard Cite

The objective of this research is to investigate the effect of yeast (Saccharomyces cerevisiae) adding and roughage-to-concentrate ratio (R:C ratio) on nutrients utilization, rumen fermentation efficiency, microbial protein synthesis, and protozoal population in Thai native beef cattle. Four Thai native beef cattle, weighing an average of 120 ± 10 kg live weight, were randomly assigned to four dietary treatments using a 2 × 2 factorial arrangement in a 4 × 4 Latin square design. Factor A was the level of roughage-to-concentrate ratio (R:C ratio) at 60:40 and 40:60; factor B was the levels of live yeast (LY) supplementation at 0 and 4 g/hd/d; urea–calcium-hydroxide-treated rice straw were used as a roughage source. Findings revealed that total intake and digestibility of dry matter (DM), organic matter (OM), and crude protein (CP) were increased (p < 0.05) by both factors, being greater for steers fed a R:C ratio of 40:60 supplemented with 4 g LY/hd/d. Ruminal ammonia nitrogen, total volatile fatty acid (VFA), and propionate (C3) were increased (p < 0.05) at the R:C ratio of 40:60 with LY supplementation at 4 g/hd/d, whereas rumen acetate (C2) and the C2 to C3 ratio were decreased (p < 0.05). With a high level of concentrate, LY addition increased total bacterial direct counts and fungal zoospores (p < 0.05), but decreased protozoal populations (p < 0.05). High-concentrate diet and LY supplementation increased nitrogen absorption and the efficiency of microbial nitrogen protein production. In conclusion, feeding beef cattle with 4 g/hd/d LY at a R:C ratio of 40:60 increased C3 and nutritional digestibility while lowering protozoal population.

show abstract

Section: Resultssupporting

confidence: 93%

The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle

Phesatcha

Wanapat

Cherdthong

2021

Animals

View full text Add to dashboard Cite

show abstract

“…Many studies have confirmed that the metabolism in the cecum of meat rabbits is similar to that in the rumen, and there are cocci similar to the rumen in the cecum of meat rabbits ( 34 ). Ruminococcaceae can degrade cellulose and hemicellulose in feed, produce butyrate by fermenting complex non-digestible polysaccharides, and plays a pivotal role in the maintenance of intestinal health ( 35 ). This study, may explain the significant increase of butyric acid content in AM- and PV-treated meat rabbits.…”

Section: Discussionmentioning

confidence: 99%

Gut Microbiota Modulate Rabbit Meat Quality in Response to Dietary Fiber

et al. 2022

View full text Add to dashboard Cite

Antibiotics are widely used in gastrointestinal diseases in meat rabbit breeding, which causes safety problems for meat products. Dietary fiber can regulate the gut microbiota of meat rabbits, but the mechanism of improving meat quality is largely unknown. The objective of this study was to evaluate the effects of adding different fiber sources to rabbit diets on the growth performance, gut microbiota composition, and muscle metabolite composition of meat rabbits. A total of 18 New Zealand white rabbits of similar weight (40 ± 1 day old) were randomly assigned to beet pulp treatment (BP), alfalfa meal treatment (AM), and peanut vine treatment (PV). There were 6 repeats in each treatment and all were raised in a single cage. The predictive period was 7 days and the experimental period was 40 days. The results revealed that AM and PV supplementation increased growth performance, slaughter performance, and intestinal development of meat rabbits compared with the BP treatment, and especially the effect of AM treatment was better. The content of butyric acid was increased in PV and AM treatments compared with the BP treatment. The expression of mitochondrial biosynthesis genes of liver, cecum, and muscle showed that AM treatment increased gene expression of CPT1b compared to the BP treatment. In addition, AM and PV treatments significantly increased the microbial diversity and richness compared with BP treatment, and their bacterial community composition was similar, and there were some differences between AM and PV treatments and BP treatment. Metabonomics analysis of muscle showed that AM treatment significantly increased amino acid and fatty acid metabolites compared with BP treatment, which were mainly concentrated in energy metabolism, amino acid metabolism, and fatty acid regulation pathways. Furthermore, through correlation analysis, it was found that there was a significant correlation between rumenococci in the cecum and amino acid metabolites in the muscle. Overall, these findings indicate that AM may affect the body's health by changing its gut microbiota, and then improving meat quality, and the intestinal–muscle axis provides a theoretical basis.

show abstract

“…The PCR conditions for amplifying the 16S rDNA gene are as follows: initial denaturation at 95°C for 1 min, followed by 27 cycles of denaturation at 95°C for 30 s, annealing at 55°C for 30 s, extension at 72°C for 45 s, and a final extension at 72°C for 10 min. The PCR products were detected using agarose gel electrophoresis (2% gel), purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), and quantified using the Quantus™ Fluorometer (Promega, USA) ( Chen et al., 2021 ). The purified amplicons were sequenced using the Illumina MiSeq PE300/NovaSeq PE250 platform (Illumina, San Diego, CA, USA), according to the standard protocol of Meggie Biological Co., Ltd. (Shanghai, China) ( Chen et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The PCR products were detected using agarose gel electrophoresis (2% gel), purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA), and quantified using the Quantus™ Fluorometer (Promega, USA) ( Chen et al., 2021 ). The purified amplicons were sequenced using the Illumina MiSeq PE300/NovaSeq PE250 platform (Illumina, San Diego, CA, USA), according to the standard protocol of Meggie Biological Co., Ltd. (Shanghai, China) ( Chen et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Effect of Early Pathogenic Escherichia coli Infection on the Intestinal Barrier and Immune Function in Newborn Calves

Wang

Simujide

Areum

et al. 2022

Front. Cell. Infect. Microbiol.

Self Cite

View full text Add to dashboard Cite

We studied the effect of early pathogenic Escherichia coli infection on newborn calves’ intestinal barrier and immune function. A total of 64 newborn Holstein male calves (40–43 kg) were divided into two groups: normal (NG) and test (TG), each with 32 heads. At the beginning of the experiment, the TG calves were orally administered pathogenic E. coli O1 (2.5 × 1011 CFU/mL, 100 mL) to establish a calf diarrhea model. In contrast, the NG calves were given the same amount of normal saline. During the 30 d trial period, the feeding and management of the two groups remained constant. Enzyme-linked immunosorbent assay, quantification PCR, and high-throughput 16S rRNA sequencing technology were used to detect indicators related to the intestinal barrier and immune function in the calf serum and tissues. Pathogenic E. coli O1 had a significant effect on calf diarrhea in the TG; it increased the bovine diamine oxidase (P < 0.05) and endotoxin levels in the serum and decreased (P < 0.05) the intestinal trefoil factor (P < 0.05), Occludin, Claudin-1, and Zonula Occludens 1 (ZO-1) levels in the colon tissue, as well as downregulated the mRNA expression of Occludin, Claudin-1,and ZO-1 in the colon mucosa, leading to increased intestinal permeability and impaired intestinal barrier function. Additionally, pathogenic E. coli had a significant impact on the diversity of colonic microbial flora, increasing the relative abundance of Proteobacteria at the phylum level and decreasing the levels of Firmicutes and Bacteroides. At the genus level, the relative abundance of Escherichia and Shigella in the TG increased significantly (P < 0.05), whereas that of Bacteroides, Butyricicoccus, Rikenellaceae_RC9_gut_group, Blautia, and Lactobacillus was significantly decreased (P < 0.05). In addition, the level of IL-6 in the serum of the TG calves was significantly increased (P < 0.05), whereas the IL-4 and IL-10 levels were significantly decreased (P < 0.05), compared to those in the NG calves. Thus, pathogenic E. coli induced diarrhea early in life disrupts intestinal barrier and impairs immune function in calves.

show abstract

Effects of dietary forage to concentrate ratio on nutrient digestibility, ruminal fermentation and rumen bacterial composition in Angus cows

Cited by 58 publications

References 67 publications

The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle

The Effect of Yeast and Roughage Concentrate Ratio on Ruminal pH and Protozoal Population in Thai Native Beef Cattle

Gut Microbiota Modulate Rabbit Meat Quality in Response to Dietary Fiber

Effect of Early Pathogenic Escherichia coli Infection on the Intestinal Barrier and Immune Function in Newborn Calves

Contact Info

Product

Resources

About