Effects of dietary Nannochloropsis sp. powder and lipids on the growth performance and fatty acid composition of larval and postlarval kuruma shrimp, Marsupenaeus japonicus

Abstract

The current study evaluated the effects of fish oil replacement with eicosapentaenoic acid-enriched single-cell microalgae in the diets of larval and postlarval kuruma shrimp. Experimental diets containing different level of Nannochloropsis sp. powder (10, 40, 70 g/kg) and lipids (2, 8, 10, 14, 35, 58 g/kg) were evaluated. The substitution of fish oil with algal powder significantly improved shrimp growth compared to that in the control group, with the highest final body weight recorded at 70 g/kg in larvae and 40 g/kg in postlarvae. Larvae fed algal lipids with a dry weight percentage in the diet of greater than 10 g/kg showed significantly lower performance than those in the control group. The neutral lipids in the tissue of the postlarvae that had been fed algal lipids had an improved fatty acid profile, as the content of highly unsaturated fatty acids increased compared to that in the other groups. The content of docosahexaenoic acid increased in the tissue, indicating the possible occurrence of the metabolism and accumulation of Nannochloropsis sp. essential fatty acids. Algal powder could replace FO up to 140 g/kg in shrimp diets without compromising growth and FA profiles.

The nucleolus as a genomic safe harbor for strong gene expression in Nannochloropsis oceanica

“…This novel tool represents an outstanding asset for genetic and metabolic engineering of an industrially attractive microalga (Li et al, 2019). Moreover, it can be used to transform Nannochloropsis into a viable source of functional (aquaculture) feed (Sarker et al, 2018(Sarker et al, , 2020Adissin et al, 2020). A list of potential strategies includes tailoring the algal proteome to dietary requirements (Herman and Schmidt, 2016), enrichment for bioactive proteins, including antimicrobial peptides and immunostimulants (Falco et al, 2012), or design of a feed-based vaccine (Clarke et al, 2013;Herman and Schmidt, 2016).…”

“…Microalgae are at the interface of industrial fermentation and agriculture. Their high protein content, nutritional value, and phototrophic growth rate, together with the prospect of cultivation on non-arable land, make them a potential replacement for traditional agricultural products in food and feed, as well as platforms for the biomanufacturing of a variety of desirable products for the chemical and pharmaceutical sector (Lamminen et al, 2019;Adissin et al, 2020;Sarker et al, 2020). Microalgae naturally produce many compounds of interest, including highvalue lipids, vitamins, pigments, and sterols (Levasseur et al, 2020).…”

Effects of adding rotifers on the water quality, plankton composition and growth of Pacific white shrimp,Litopenaeus vannameijuvenile, when cultured with biofloc technology

“…To improve the nutritional content of rotifers, microalgae are used in various forms (frozen, dry, concentrated or fresh) (Kotani et al, 2010; Seychelles et al, 2009; Tzovenis et al, 2004). One widely used microalgae is Nannochloropsis sp., which contains substantial amounts of EPA (Adissin et al, 2019). Other forms of enrichment include the use of baker's yeast ( Saccharomyces cerevisiae ) (Penglase et al, 2011), microcapsules (Langdon, 2003) and emulsions (Haché & Plante, 2011) rich in lipids.…”

The Application of Single-Cell Ingredients in Aquaculture Feeds—A Review

“…The use of microalgal SCP and SCO has been particularly active in shrimp nutrition and has been examined in a range of studies in the past decade [124][125][126][127][128][129][130][131][132][133][134].…”

“…The effect of a Nannochloropsis SCP on post-larval kuruma shrimp (Marsupenaeus japonicus) performance was evaluated by Adissin et al [133]. The SCP was included at various inclusion levels up to 70 g/kg.…”