Effects of dietary intake of heat-inactivated <i>Lactobacillus gasseri</i> CP2305 on stress-induced behavioral and molecular changes in a subchronic and mild social defeat stress mouse model

Abstract

The intestinal ecosystem is involved in the pathogenesis of mood disorders such as depression. Intestinal microbes can affect the central nervous system through the gut-brain axis, which raises the possibility of using probiotics for preventing depression. In this study, we examined the effect of heat-inactivated Lactobacillus gasseri CP2305 (CP2305) in a subchronic and mild social defeat stress (sCSDS) mouse model. sCSDS suppressed food intake. However, dietary CP2305 intake rescued it, suggesting that CP2305 improved the decreased appetite in sCSDS mice. sCSDS did not alter the gene expression of brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3 in the hippocampus. However, dietary CP2305 provided following sCSDS increased the gene expression of these neurotrophins in the hippocampus. These findings suggest that CP2305 supplementation would aid in preventing psychosocial stress-induced disorders.

Effect of Probiotic Bifidobacterium bifidum TMC3115 Supplementation on Psychosocial Stress Using a Sub-Chronic and Mild Social Defeat Stress in Mice

“…Secondly, the TMC3115 supplementation alleviated the polydipsia-like symptom (excessive water intake) during the sCSDS period ( Figure 2 D). A polydipsia-like symptom is the most characteristic phenotype of the sCSDS model [ 17 , 21 , 22 , 23 ]. Although this phenotype is caused by stress, the mechanism behind this is still unknown.…”

“…It has been reported that microbiota in the cecum and feces of mice subjected to the sCSDS model is different from that of non-stressed control mice [ 19 ]. Therefore, some studies assessed the effects of probiotic or postbiotic [ 20 ] lactobacilli, but not bifidobacteria, on stress-induced behavioral, molecular, and/or intestinal microbiota changes using the sCSDS model [ 21 , 22 , 23 ].…”

Functional bacterial cultures for dairy applications: Towards improving safety, quality, nutritional and health benefit aspects

“…Remarkably, some postbiotics composed of heat‐inactivated lactobacilli retain their neuro activity. In this regard, heat‐killed lactobacilli were able to favour sociability and decrease corticosterone levels in mice (Warda et al, 2019), and dietary intake of heat‐killed Lactobacillus gasseri CP2305 improved appetite in a mouse model and triggered an increase in the gene expression of neurotrophins in the hippocampus (Toyoda et al, 2020). In relation to bifidobacteria with a potential role in the gut‐brain axis, some studies have been focused on its potential to impact on sleep behaviour or depression, although the molecules that are behind the dialogue with the nervous system have not always been characterized.…”

Consecutive intra-gingival injections of lipopolysaccharide and butyric acid to mice induce abnormal behavior and changes in cytokine concentrations

“…The concentrations of neurotransmitters such as acetylcholine (Ach), adrenaline, dopamine (DA), gamma amino butyric acid (GABA), kynurenic acid (KA), 3-hydroxykynurenine (3-HK), serotonin (5-HT), tryptophan (Trp), and tyrosine (Tyr) in blood plasma and brain tissues were measured using an ultrapressure liquid chromatography apparatus equipped with a binary solvent manager, an autosampler, a column heater, and tandem mass spectrometry (Acquity TQD UPLC-MS/MS; Nihon Waters, Tokyo, Japan). The measurement procedure was the same as that previously described [17].…”