Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

Zheng, Keke; Zhu, Xiaoming; Han, Dong; Yang, Yunxia; Wu, Lei; Xie, Shouqi

doi:10.1016/j.aquaculture.2009.11.028

Cited by 90 publications

(67 citation statements)

References 41 publications

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“…This is similar to other studies that showed that survival increases with crude lipid up to an asymptote (Chou et al, 2001;Zheng et al, 2010). Interestingly, we found no relationship between survival and levels of individual fatty acids in the diet despite previous research suggesting that regulation of stress by essential fatty acids could affect survival (Watanabe 1993, Izquierdo 2005.…”

Section: Discussionsupporting

confidence: 40%

Linking fatty acids in the diet and tissues to quality of larval southern flounder (Paralichthys lethostigma)

Oberg

Fuiman

2015

Journal of Experimental Marine Biology and Ecology

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Section: Discussionsupporting

confidence: 40%

Linking fatty acids in the diet and tissues to quality of larval southern flounder (Paralichthys lethostigma)

Oberg

Fuiman

2015

Journal of Experimental Marine Biology and Ecology

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“…The activity of phosphoenolpyruvate carboxykinase (PEPCK, EC 4.1.1.32) was determined by the method of Lee, Hebda, and Nowak (). Total lipase (TLP) including lipoprotein lipase (LPL, EC 3.1.1.34) and hepatic lipase (HL, EC 3.1.1.3) was measured as described previously (Zheng et al, ). The activities of HK, PEPCK and TLP were determined spectrophotometrically using diagnostic reagent kits (A077–1, A131 and A067, respectively).…”

Section: Methodsmentioning

confidence: 99%

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

et al. 2019

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Japanese flounder juveniles (initial body weight: 7.12 ± 0.02 g) were fed three diets containing 0, 120 and 200 g/kg of carbohydrates (C0, C120 and C200, respectively) for 10 weeks. Results showed that higher dietary carbohydrate intake enabled further deposition of glycogen and lipids in liver and muscle. The mRNA levels of glucokinase (gck), phosphofructokinase (pfkl) and hexokinase (HK) activity involved in glycolysis were significantly up‐regulated in C120 (p < .05) but showed no further up‐regulation except for gck in C200. Besides, the gluconeogenic phosphoenolpyruvate carboxykinase 1 (pck1) mRNA levels were down‐regulated significantly in fish fed the C120 (p < .05). However, further increase in dietary carbohydrate levels increased the mRNA levels and activities of enzymes involved in gluconeogenesis and lipolysis (p < .05). Additionally, plasma glucose remained unchanged in C120 (p > .05) but significantly increased in C200 group (p < .05). In conclusion, Japanese flounder was able to use carbohydrates efficiently through regulation of glucose and lipid metabolism when dietary carbohydrate was not higher than 120 g/kg, while 200 g/kg of dietary carbohydrate caused the deregulation of glucose homoeostasis.

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“…To confirm the mRNA expression of the significant DEG analyses, 11 unigenes were selected and quantified using qRT‐PCR, including glycine dehydrogenase ( gldc ), maltase‐glucoamylase , intestinal‐like ( mgam ), glucose phosphate isomerase ( gpi ), ectonucleotide pyrophosphatase/phosphodiesterase 7 ( enpp7 ), glutamate dehydrogenase ( glud ), long chain fatty acid CoA ligase 5 ( acsl5 ), glucose‐6‐phosphatase catalytic subunit ( g6pc ), diacylglycerol O‐acyltransferase 2 ( dgat2 ), nitric oxide synthase 2 ( nos2 ), fatty acid‐binding protein , intestinal‐like ( fabp2 ), and apolipoprotein A‐I ( apoa1 ). The β ‐actin was used to obtain the 200‐bp fragment as a reference gene for calculating the relative expression levels of the 11 unigenes (Zheng et al., ). The paired primers were listed in supporting information Table .…”

Section: Methodsmentioning

confidence: 99%

Comparative transcriptome sequencing of the intestine reveals differentially expressed genes in Pelteobagrus vachellii

et al. 2018

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To better understand the molecular mechanisms underlying digestion and absorption in the intestine of the darkbarbel catfish (Pelteobagrus vachellii), we studied the intestinal transcriptome between day and night‐time. In this study, two triplicate groups of P. vachellii (1.05 ± 0.36 g) were fed either at 08:00 (D24 group), control) or 20:00 (W24 group) with the same amount of commercial feed. A significant increase of the apparent digestibility (AD) of protein and lipid was observed in the night group (p < 0.05). Comparison of gene expression levels revealed that 1,125 genes were significantly up‐regulated and 364 genes were significantly down‐regulated in the night group. Among them, 54 digestion‐ and absorption‐related genes, apolipoprotein A‐I, trypsin 1b, solute carrier family 26 member 6 b, sodium/glucose cotransporter 1, cholecystokinin B, maltase‐glucoamylase, diacylglycerol O‐acyltransferase 2, ATP‐binding cassette subfamily A member 1‐like and phospholipase A2, etc., were up‐regulated. Additionally, in the night group, 18 carbohydrate metabolism‐related genes, 17 protein metabolism‐related genes and 16 lipid metabolism‐related genes were up‐regulated, including fatty acid‐binding protein, diacylglycerol O‐acyltransferase 2, adipose triglyceride lipase, phospholipase A2, glucose phosphate isomerase, glycogen phosphorylase, arginase‐2, d‐amino‐acid oxidase, glutamate dehydrogenase, glycine n‐acyltransferase, tryptophan 2,3‐dioxygenase, kynureninase, cysteine dioxygenase type 1 and glycine dehydrogenase. The results indicated that a shift in feeding time from day to night may improve the digestion and absorption of proteins and lipids. Moreover, the amino acid metabolism, triglyceride synthesis, gluconeogenesis may be also influenced by the shifted feeding time.

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Effects of dietary lipid levels on growth, survival and lipid metabolism during early ontogeny of Pelteobagrus vachelli larvae

Cited by 90 publications

References 41 publications

Linking fatty acids in the diet and tissues to quality of larval southern flounder (Paralichthys lethostigma)

Linking fatty acids in the diet and tissues to quality of larval southern flounder (Paralichthys lethostigma)

Molecular adaptations of glucose and lipid metabolism to different levels of dietary carbohydrates in juvenile Japanese flounderParalichthys olivaceus

Comparative transcriptome sequencing of the intestine reveals differentially expressed genes in Pelteobagrus vachellii

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