Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens

Zhang, B.; Yang, Xin; Guo, Yuming; Long, Fangyu

doi:10.1017/s1751731111001066

Cited by 20 publications

(25 citation statements)

References 37 publications

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“…Barter et al (2010) reported that a link may exist between the changes in HDL-C and TG, but they found no apparent relationship between LDL-C reduction and HDL-C increase. However, Zhang et al (2011) and Zhao et al (2013) observed that diet containing Clostridium butyricum and Enterococcus faecium exerted no effect on the concentrations of TG, TC, HDL-C, and LDL-C in broiler sera. Meanwhile, the serum INS levels were enhanced by C .…”

Section: Discussionmentioning

confidence: 98%

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

et al. 2017

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Numerous studies have focused on the beneficial effects of probiotics in animals. Even so, additional information should be obtained about the mechanisms by which a useful probiotic strain successfully exerts such beneficial effects. In this study, we evaluated the effect of the dietary supplementation of both live and disrupted Lactobacillus johnsonii (LJ) strain BS15 in broilers at different ages. Specifically, growth performance, lipid metabolism, gut microbiota, intestinal development, and digestive ability of the broilers were assessed. A total of 180 1-day-old Cobb 500 chicks were randomly distributed into three groups. These chicks were fed diets supplemented with 1 × 106 colony-forming units (cfu) LJ per gram of feed (LJ group); 1 × 106 cfu disrupted LJ per gram of feed (D-LJ group); and de Man, Rogosa, and Sharpe liquid medium (control group), respectively, throughout a 42-day experimental period. The results demonstrated that LJ supplementation of feed had a positive effect on the average daily gain and starter feed conversion ratio. In addition, LJ supplementation of feed decreased serum triglyceride and low-density lipid cholesterol levels, as well as abdominal fat deposition. LJ also reduced the mRNA levels of lipoprotein lipase in adipose tissue and stearoyl-CoA desaturase-1 in the liver. LJ diminished the mRNA quantities of the sterol regulatory element binding protein-1c and fatty acid synthase, as well as increased the level of serum high-density lipid cholesterol. LJ increased the mRNA quantities of peroxisome proliferator-activated receptor α, acyl-CoA oxidase in the liver, and carnitine palmitoyltransferase-1. LJ also improved the intestinal development and digestive ability mainly by increasing the villus height/crypt depth ratio in the ileum. The probiotic increased the levels of epidermal growth factor and insulin-like growth factor-1, as well as the activities of trypsin and lipase in the jejunum and ileum. LJ exerted beneficial effects on the intestinal flora. Specifically, LJ markedly enhanced the population of Bacteroidetes and Lactobacillus spp. Moreover, the probiotic reduced the population of Enterobacteriaceae and the Firmicutes/Bacteroidetes ratio. Slight changes caused by disrupted LJ were detected. These findings indicated that live LJ supplementation may promote growth performance and lower fat deposition in broilers.

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Section: Discussionmentioning

confidence: 98%

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

et al. 2017

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“…PPAR-α, as a member of nuclear receptors, is highly expressed in the liver and plays an important role in regulating glucose and lipid metabolism or enhancing the related-gene expression in fatty acid oxidation (Lee et al, 1995;Zhang et al, 2011;Huang et al, 2013). LPL, on the other hand, catalyzes triglycerides from circulating chylomicron and very low-density lipoprotein to generate fatty acids and glycerol for tissue utilization or storage (Zhao et al, 2013).…”

Section: Effect Of Cottonseed Meal Fermented With Yeast On the Lipid-mentioning

confidence: 99%

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

Nie

et al. 2015

Rev. Bras. Cienc. Avic.

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Fermented cottonseed meal (FCSM) is widely used in poultry diets in China. This study was conducted to investigate the effect of FCSM on lipid-related gene expression in broilers. Initially, 180 broiler chickens (21-days-old, equal number of males and females) were randomly divided into three groups, with six pens per group and 10 birds per pen. The chickens in the control group were fed a diet containing unfermented cottonseed meal, and those in the treatment groups were fed with diets including either CSM fermented by Candida tropicalis (Ct group) or CSM fermented by Candida tropicalis plus Saccharomyces cerevisae (Ct-Sc group) until 64 days old. The results revealed that, compared with the control group (p<0.05), the mRNA expression of peroxisome proliferator-activated receptor alpha (PPAR-α) and lipoprotein lipase (LPL) were upregulated in the livers of Ct-Sc males. The expression of PPAR-α was also upregulated in the livers of Ct females. The expression levels of acetyl CoA carboxylase (ACC) and LPL in the liver of males and the expression of PPAR-α in the liver of females were significantly different between the Ct and Ct-Sc groups (p<0.05). However, gene expressions of fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) in the liver were not altered when the broilers were fed FCSM-supplemented diets (p>0.05). Likewise, the expressions of peroxisome proliferator-activated receptor gamma (PPAR-γ) and LPL in the abdominal fat were not altered by the FCSMsupplemented diets (p>0.05). The results in this study indicate that CSM fermented by Candida tropicalis and Saccharomyces cerevisiae effectively regulated the genes involved in fatty acid β-oxidation and triglyceride hydrolysis in male broiler chickens. Furthermore, the effects of the FCSM-supplemented diets were significantly different between bird sexes and between yeast strains used in the fermentation process.

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“…Apart from being an important macronutrient, dietary fat has recently gained much prominence for its role in regulating gene expression in poultry (Royan et al 2011;Zhang et al 2011;Jing et al 2013). LPIN1, LPIN2, WDTC1/ADIPOSE, and △-6 fatty acid desaturase (FADS2) were reported as lipid metabolism genes in human and mouse.…”

Section: Introductionmentioning

confidence: 99%

Effects of the replacement of corn oil with linseed oil on fatty acid composition and the expression of lipogenic genes in broiler chickens

Chen¹,

Zhao²,

Yan³

et al. 2014

CZECH J ANIM SCI

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ABSTRACT:The effect of dietary 18:2n-6/18:3n-3 ratio (by the replacement of corn oil with linseed oil) on n-3 polyunsaturated fatty acids (PUFA) enrichment in breast muscle of broiler chickens and the expression of lipogenic genes were investigated. Broiler chickens were fed ad libitum with diets containing 5% corn oil (CO), 3.75% corn oil + 1.25% linseed oil (CL1), 2.5% corn oil + 2.5% linseed oil (CL2), and 5% linseed oil (LO) based on the basic diets, respectively. Dietary 18:2n-6/18:3n-3 ratio did not affect 42-day body weight and 0-42-day feed conversion efficiency (feed/gain, P > 0.05) of broiler chickens, however, 5% linseed oil significantly increased 0-21-day feed conversion efficiency (feed/gain, P < 0.05) and decreased breast muscle weight (by 16%, P < 0.05) of broiler chickens. With the decrease of dietary 18:2n-6/18:3n-3 ratio, the enrichment of total n-3 PUFA, 18:3n-3, 20:5n-3, and 22:5n-3 increased linearly (P < 0.01), while the enrichment of total n-6 PUFA and 18:2n-6 decreased linearly (P < 0.01) in breast muscle of broiler chickens. Dietary corn oil increased the enrichment proportion of 20:4n-6 in a dosage-independent manner. Replacing 1.5% corn oil with linseed oil increased the enrichment proportion of 22:6n-3 (P < 0.05), but continuing to increase dietary linseed oil could not further elevate its deposition. Real-time quantitative RT-PCR was used to determine the expression of the mRNA levels of related genes. Dietary PUFA had insignificant effect on the expressions of LPIN2, WD, and tetratricopeptide repeats 1 (WDTC1) and △-6 fatty acid desaturase (FADS2) in both breast muscle and abdominal fat. The effect of dietary PUFA on the expression of LPIN1 gene showed clear tissue dependence. Equivalent adding of corn oil and linseed oil could up-regulate the mRNA level of LPIN1 in abdominal fat (P < 0.01). This study demonstrated that decreasing dietary 18:2n-6/18:3n-3 ratio promoted the deposition of desirable n-3 long chain PUFA in the edible tissue and influenced the expression of LPIN1 in a tissue-dependent manner.

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Effects of dietary lipids and Clostridium butyricum on serum lipids and lipid-related gene expression in broiler chickens

Cited by 20 publications

References 37 publications

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

Effects of the replacement of corn oil with linseed oil on fatty acid composition and the expression of lipogenic genes in broiler chickens

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