Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm

Samadian, Farhad; Towhidi, Armin; Rezayazdi, K.; Bahreini, Majid

doi:10.1017/s1751731110001308

Cited by 79 publications

(62 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There have been several studies with regards to the supplementation of fish oil into the rooster (10), boar (12) goat (29), sheep (30) and turkey (31) diet. The results revealed that the diet supplemented with fish oil led to an increase in sperm progressive motility.…”

Section: Discussionmentioning

confidence: 99%

Combined n-3 Fatty Acids and α-Tocopherol Supplementation Improved the Ovine Sperm Cryosurvival

et al. 2013

View full text Add to dashboard Cite

Background: Spermatozoa preservation is an approach to improve the fertility rate to pass on the valuable genetic material from sire to their offspring. During the last 20 years, reproductive biotechnologists have focused on the approaches that improve spermatozoa cryosurvival. One of the possible mechanisms is supplementation in semen extender. Objectives: The aim of this study was to evaluate the combined effects of healthy ram semen with n-3 fatty acids and α-tocopherol (Vitamin E) on freezing ability and fatty acid (FA) content of sperm cell. Materials and Methods: Semen collection was performed on six mature Zandi rams by an artificial vagina. In the present study, two experiments were carried out. In Experiment 1, the specimen quality was assessed. Then the samples were pooled. The pooled specimens were allocated into 12 groups, in a 3 × 4 factorial design, including four levels of n-3 FA (0, 0.1, 1, 10 ng.mL -1 ) and three levels of α-tocopherol (0. 0.1, 0.2 mM). Then sperm critical characteristics such as proportion of motile sperm, progressive motile sperm, viable and abnormal sperms were measured. Furthermore, after freezing-thawing procedure, the recovery rate was considered as a vital indicator of semen quality. After thawing, the highest progressive motility was obtained when treated with 0.1 mM α-tocopherol and 1 ng.mL -1 n-3 FA. So, the second experiment was designed to measure the content of FA in specimens that fortified with 0.1 mM α-tocopherol, 1 ng.mL -1 n-3 FA and also in groups without α-tocopherol and FA. Results: The data showed that before freezing, docosahexaenoic acid (DHA) level of sperm was increased when the FA introduced into extender (P ≤ 0.01). On the other hand, in the FA group, the n-3 FA and polyunsaturated fatty acid content were significantly higher compared with n-6 FA and saturated fatty acid level. However, in other groups, there were no significant alteration in the overall proportion of n-3 FA and n-6 FA were recorded (P ≤ 0.01). conclusions: It was concluded that the cryosurvival of ram semen could be improved by adding DHA along with alfa-tocopherol as an antioxidant.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined n-3 Fatty Acids and α-Tocopherol Supplementation Improved the Ovine Sperm Cryosurvival

et al. 2013

View full text Add to dashboard Cite

show abstract

“…In most mammals, fatty acid composition of their spermatozoa is characterized by very high proportions of n-3 PUFAs (Kelso et al, 1997;Samadian et al, 2010). Docosahexaenoic (DHA) has been reported to be the predominant PUFAs within the sperm plasma membrane (Maldjian et al, 2005), but during the cryopreservation process, the level of PUFAs decreases significantly because of lipid peroxidation (Cerolini et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of DHA supplementation of the extender containing egg yolk and α- tocopherol on the freezability and post-thawing fertility of ram semen

Abdi‐Benemar

Jafaroghli

Khalili³

et al. 2015

Small Ruminant Research

View full text Add to dashboard Cite

“…Many researchers have assessed the effect of fish oil addition on semen quality parameters in domestic animals. They have reported that fish oil caused increments in sperm motility and reductions in abnormal sperm in boars [9] , improved fertility in turkeys [10] as well as increased total of sperm count in rams [11] . On the other hand, the addition of fish oil as a source of omega-3 fatty acids to diet of boars has been demonstrated to possess no significant improvement effect on the quality of frozen-thawed sperm [12] .…”

Section: Introductionmentioning

confidence: 99%

Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi

Malik¹,

Syarifdjaya²,

Gunawan³

et al. 2017

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Citation of This ArticleMalik A, Syarifdjaya M, Gunawan A, Erlina S, Jaelani A, Wibowo DB: Effect of fish oil addition to the skim milk-egg yolk extender on the quality of frozen-thawed Bali bull spermatozoa. Kafkas Univ Vet Fak Derg, 23 (4): 651-654, 2017651-654, . DOI: 10.9775/kvfd.2017 AbstractThis study was conducted to investigate the effect of fish oil addition to the semen extender on post-thaw quality of Bali bull spermatozoa. Fish oil was added at the doses of 0 (control), 50, 100, 150 and 200 mg/100 mL to the skim milk-egg yolk extender. Supplementation of fish oil at the dose of 100 mg/mL significantly (P<0.05) increased both live sperm rate and motility, but its dose of 200 mg/mL caused a significant (P<0.05) decrease in the live sperm rate when compared with the control group. A significant (P<0.05) increment was observed in abnormality rate of frozen-thawed spermatozoa in comparison with the control group when all doses of fish oil except 50 mg/100 mL were added. In conclusion, addition of 100 mg fish oil to 100 mL of skim milk-egg yolk extender could be beneficial for the improvement of the quality of Bali bull spermatozoa after freeze-thawing. Keywords: Bull semen, Fish oil, Cryopreservation, Live sperm, Motility Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi ÖzetBu çalışma sperma sulandırıcısına katılan balık yağının Bali boğa spermatozoonlarının çözdürme sonrası kalitesi üzerine etkisini araştırmak amacıyla yapıldı. Süt tozu-yumurta sarısı sulandırıcısına 0 (kontrol), 50, 100, 150 ve 200 mg/100 mL dozunda balık yağı ilave edildi. 100 mg/100 mL balık yağı ilavesi kontrol grubu ile karşılaştırıldığında hem canlı sperm oranını hem de motiliteyi önemli derecede (P<0.05) artırdı, ancak 200 mg/100 mL'lik doz canlı sperm oranında önemli (P<0.05) bir azalmaya neden oldu. 50 mg/100 mL'lik doz hariç balık yağının tüm dozları ilave edildiğinde kontrol grubuna kıyasla dondurulmuş-çözdürülmüş spermatozoonların anormallik oranında önemli (P<0.05) bir artış gözlendi. Sonuç olarak, süt tozu-yumurta sarısı sulandırıcısının 100 mL'sine 100 mg balık yağı ilavesi Bali boğa spermatozoonlarının dondurma-çözdürme sonrası kalitesinin artırılması için faydalı olabilir.

show abstract

Effects of dietary n-3 fatty acids on characteristics and lipid composition of ovine sperm

Cited by 79 publications

References 30 publications

Combined n-3 Fatty Acids and α-Tocopherol Supplementation Improved the Ovine Sperm Cryosurvival

Combined n-3 Fatty Acids and α-Tocopherol Supplementation Improved the Ovine Sperm Cryosurvival

Effects of DHA supplementation of the extender containing egg yolk and α- tocopherol on the freezability and post-thawing fertility of ram semen

Süt Tozu-Yumurta Sarısı Sulandırıcısına Balık Yağı İlavesinin Dondurulmuş-Çözdürülmüş Bali Boğa Spermatozoonlarının Kalitesi Üzerine Etkisi

Contact Info

Product

Resources

About