Effects of dietary probiotic (Pediococcus acidilactici) supplementation on productive performance, egg quality, and body composition in laying hens fed diets varying in energy density

Abstract

This study was conducted to determine the effect of probiotic Pediococcus acidilactici ( PA ) strain MA18/5M supplementation of diets with different dietary energy levels on productive performance, egg quality, and body composition in Hy-Line Brown hens during a 16-week period from 32 to 47 wk of age. The experimental treatments with a 2 × 2 factorial design received a 2 wheat–corn–soybean diet: a moderately low energy density diet with 2,650 kcal ME/kg ( M-LED ) and a low energy density diet based on the M-LED diet with 2,550 kcal ME/kg ( LED ), each diet without and with probiotic supplementation (M-LED, LED, M-LEDp , and LEDp , respectively). Reduced dietary energy levels had a particularly negative effect on egg weight (61.7 vs. 63.3 g; −2.6%, P < 0.001), egg mass output (1.67 vs. 1.71 kg; −2.4%, P = 0.015), and FCR (2.01 vs. 1.97 kg feed/kg egg; +2%, P = 0.028). In hens administered the LED diet, deteriorated productive performance was accompanied by greater body weight loss ( P < 0.001) and reduced abdominal fat content ( P < 0.033) as compared with the M-LED group. Dietary probiotic inclusion increased egg weight ( P = 0.015), including relative eggshell weight ( P = 0.008) and eggshell thickness ( P = 0.002) and significantly improved FCR ( P = 0.010). No interactions between the PA-based probiotic and dietary energy levels were found in any of the tested parameters. Adding the probiotic on top of the M-LED diet improved layers performance but resulted in nonbioequivalence for the egg weight, egg mass output, and FCR compared with this group without probiotic. Probiotic supplementation of the LEDp diet improved all performance parameters except for egg weight. As a result, the laying rate, egg mass output, daily feed intake, and FCR in the LEDp treatment were bioequivalent to those noted in the M-LED group without the probiotic. The results of a bioequivalence test suggest that a low energy diet fed to laying hens promoted a probiotic response to improve energy utilization by birds.

Probiotics (Direct-Fed Microbials) in Poultry Nutrition and Their Effects on Nutrient Utilization, Growth and Laying Performance, and Gut Health: A Systematic Review

“…Studies found that probiotic supplementation has effects on egg production [ 29 , 33 , 34 , 45 , 69 ]. The following variables were considered to evaluate egg production performance: ADFI, ADG, FCR, the number of the eggs laid, egg weight, specific gravity, serum and egg yolk cholesterol, and serum triglyceride.…”

“…Improves laying performance and modulates the gut microbiota. [44][45][46] Propionibacterium acidipropionici…”

In-vitro investigation of cholesterol removal, ß-galactosidase synthesis, antioxidant, and antidiabetic potential of probiotic organisms

“…When the treatment was continued for 24 h, it resulted in 52 ± 0.02% removal. In a similar way, Mikulski et al [33] have observed 12.33% cholesterol removal from egg yolk by Pediococcus acidilactici. With a step ahead, Lokapirnasari et al [34] aimed to study the combitorial effect of Bifidobacterium spp.…”

Performance of distinct microbial based solutions in a Campylobacter infection challenge model in poultry

“…To address global bans on the use of AGPs, the poultry industry has explored the use of probiotics, natural microbes that confer a beneficial effect on their host [ 12 , 13 ]. Single strain probiotic species including species of Streptococcus , Bacillus , Bifidobacterium , Enterococcus , Lactobacillus, Pediococcus and Saccharomyces , have been shown to have beneficial effects on broiler performance, laying hens, modulation of intestinal gut microbiota and pathogen inhibition [ 13 – 16 ]. However, probiotics have shown varying results between products and farms [ 17 ], limiting our understanding of their specific impact on the composition of the gut microbiome.…”