Effects of Dietary Resveratrol Supplementation on Growth Performance and Anti-Inflammatory Ability in Ducks (Anas platyrhynchos) through the Nrf2/HO-1 and TLR4/NF-κB Signaling Pathways

Abstract

The aim of this study was to explore the effect of dietary resveratrol on the growth performance and anti-inflammatory mechanism in ducks. A total of 280 one-day-old specific pathogen-free male ducklings (Anas platyrhynchos) with an average body weight of 35 ± 1 g were randomly divided into two dietary treatment groups with different supplementation levels of resveratrol for growth performance experiments: R0 and R400 (0 and, 400 mg kg−1 resveratrol, respectively). At the age of 28 days, 16 ducks were selected from each treatment group and divided into four subgroups for a 2 × 2 factorial pathological experiment: R0; R400; R0 + LPS; R400 + LPS, (0 mg kg−1 resveratrol, 400 mg kg−1 resveratrol, 0 mg kg−1 resveratrol, 400 mg kg−1 resveratrol + 5 mg lipopolysaccharide/kg body weight). The results showed that resveratrol significantly improved final body weight and average daily gain (p < 0.01) and alleviated the lipopolysaccharide-induced inflammatory response with a reduction in IL-1β and IL-6 in the plasma and the liver (p < 0.05). Resveratrol improved mRNA levels of Nrf2 and HO-1 and decreased the mRNA levels of TLR4 and NF-κB in duck liver (p < 0.05). Dietary resveratrol can improve growth performance and reduce inflammation through the Nrf2/HO-1 and TLR4/NF-κB signaling pathways in duck.

Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway

“…Zhang et al [ 11 ] revealed that the mRNA expression levels of CAT , GR , MnSOD , HO-1, and Nrf2 in the spleen of broilers under HS were increased by dietary RES, indicating that the Nrf2 signaling pathway in the spleen might be activated. Similarly, Wang et al [ 17 ] reported that Nrf2 and SOD mRNA levels in the jejunum of heat-stressed boilers could be improved by RES, which may alleviate the inhibitory effect of HS on the Nrf2 signaling pathway. The present study found that Nrf2 , HO-1 , and NQO1 mRNA in the muscle of heat-stressed broilers were remarkably improved by RES.…”

“…Moreover, our research confirmed that RES could activate the Nrf2 signaling pathway in the spleen [ 11 ] and intestinal tract [ 16 ] of broilers under HS and alleviate the oxidative damage caused by HS. A recent study in ducks revealed that RES could alleviate lipopolysaccharide-induced liver peroxidation through the enhanced expression of Nrf2 and HO-1 [ 17 ]. According to the above findings, we hypothesized that RES possibly improves the muscle antioxidant capacity of broilers subjected to HS through Nrf2 signaling pathway activation in muscle tissue, which improves meat quality.…”

Curcumin: a potential exogenous additive for the prevention of LPS‐induced duck ileitis by the alleviation of inflammation and oxidative stress

“…RNA isolation and RT‐qPCR were performed as previously reported 7 . The gene accession numbers of the ducks were obtained from the National Center for Biotechnology Information, and duck gene primers were purchased from Sangon Biotech Co. Ltd (Shanghai, China) (Supporting Information, Table S2).…”

“…Lipopolysaccharides (LPS) are the primary pathogenic components of the outer membrane of Gram‐negative bacteria; thus, LPS have become a research focus 4 . Previous studies have indicated that the LPS challenge is linked to a decrease in growth performance, oxidative stress, and inflammatory reactions in animals and humans 5‐7 . In the past century, antibiotics have been recognized as alternative therapeutic agents to combat the reduction in animal growth performance and multiple inflammatory diseases caused by bacterial infections.…”

Threonine supplementation prevents the development of fat deposition in mice fed a high-fat diet

“…Gene expression was normalized to β-actin mRNA levels in each sample and fold change was determined using the comparative method (2 −ΔΔCt ), where Ct is defined as the cycle number at which fluorescence reaches the fixed threshold. 19,20…”