2008
DOI: 10.1016/j.atherosclerosis.2007.02.007
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Effects of dietary soy protein on iliac and carotid artery atherosclerosis and gene expression in male monkeys

Abstract: Male cynomolgus macaques (n=91) consumed an isoflavone (IF)-free, atherogenic control diet containing casein/lactalbumin for five months, then were randomized to three groups: Control (n=30) continued on the control diet; Low IF (n=30) received a mixture of unmodified and IF-depleted soy protein isolate (SPI) (0.94 mg IF/g protein, approximating a human intake of 75 mg/day); and High IF (n=31) received unmodified SPI (1.88 mg IF/g protein, approximating a human intake of 150mg/ day) for 31 months. Iliac and ca… Show more

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Cited by 29 publications
(41 citation statements)
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“…The carotid arteries were removed, cleaned of adherent connective tissues, opened longitudinally, and sectioned. The common carotid arteries were subdivided into individual segments and processed as previously described for iliac arteries 22 . Briefly, adjacent segments were flash frozen in liquid nitrogen for RNA isolation and fixed in 4% paraformaldehyde for histologic examination.…”
Section: Methodsmentioning
confidence: 99%
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“…The carotid arteries were removed, cleaned of adherent connective tissues, opened longitudinally, and sectioned. The common carotid arteries were subdivided into individual segments and processed as previously described for iliac arteries 22 . Briefly, adjacent segments were flash frozen in liquid nitrogen for RNA isolation and fixed in 4% paraformaldehyde for histologic examination.…”
Section: Methodsmentioning
confidence: 99%
“…Briefly, paraffin blocks were sectioned at 5 μm and stained with Verhoeff Van Gieson as well as hematoxylin and eosin for assessment of tissue histology. Plaque area (mm2) of the carotid artery sections were determined by computer assisted histomorphometric methods using Image Pro Plus software 22 (Media Cybernetics, Inc. Silver Springs, MD).…”
Section: Methodsmentioning
confidence: 99%
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“…Quantitative real-time polymerase chain reaction (RTPCR) was used to evaluate the inflammatory cell content of thymus, spleen, and lymph node using previously described methods (Sophonsritsuk et al 2013; Walker et al 2008a, 2008b). Levels of mRNA transcripts for a specific marker associated with macrophages cluster of differentiation 68 (CD68), and four markers of T cells T-cell receptor beta, cluster of differentiation 3, 4 and 8 (TCRB, CD3, CD4, CD8) were measured in order to define the cellular composition of the tissue.…”
Section: Methodsmentioning
confidence: 99%
“…RNA quality was assessed by intensity of 28S and 18S ribosomal RNA bands on an Agilent Microanalyzer. Quantitative Taqman ® RT-PCR assays employing cynomolgus-specific primers and Fam-labeled Taqman ® MGB probes were used to quantitate mRNA steady-state levels (Sophonsritsuk et al, 2013; Walker et al, 2008a, 2008b). Aliquots of purified total RNA (~ 1–10 ug RNA) were reverse transcribed using a High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA) in combination with random hexamers to generate cDNA from which real time RT-PCR quantitative analyses were performed.…”
Section: Methodsmentioning
confidence: 99%