Effects of dietary supplementation with icariin on growth performance, antioxidant capacity and non-specific immunity of Chinese mitten crab (Eriocheir sinensis)

Zheng, Xiaochuan; Chi, Cheng; Xu, Chenyuan; Liu, Jiadai; Zhang, Caiyan; Zhang, Ling; Huang, Yeen; He, Chunyan; He, Chang; Jia, Xiaoyan; Liu, Wenbin

doi:10.1016/j.fsi.2019.04.296

Cited by 61 publications

(21 citation statements)

References 67 publications

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“…ACP is a vital hydrolytic enzyme in phagocytic lysosomes [25,26], which participates in degradation of foreign proteins, carbohydrates, and lipids [27]. Our data revealed that the ACP activity was enhanced by exposure to TD 49 at various time intervals.…”

Section: Discussionmentioning

confidence: 71%

Effect of the Algicide Thiazolidinedione 49 on Immune Responses of Bay Scallop Argopecten Irradians

Chi

Yun²,

Giri³

et al. 2019

Molecules

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The thiazolidinedione 49 (TD49) is an effective algaecide against harmful algae; however, its potential effects on the immune function of the edible bay scallop are unclear. Therefore, the present work studied the effects of TD49 on the immune response in bay scallop by evaluating activities of acid phosphatase (ACP), alkaline phosphatase (ALP), and superoxide dismutase (SOD), as well as nitric oxide (NO) levels, total protein content, and expression of immune genes (CTL-6, PGRP, PrxV, MT, and Cu/Zn-SOD) at 3–48 h post-exposure (hpe) to TD49. The activities of ACP and ALP significantly increased in TD49-treated groups at 3–24 hpe, whereas NO levels decreased significantly in 0.58 and 0.68 μM of TD49 at 6–24 hpe, after which the level was similar to that in the untreated control. Moreover, SOD activity significantly increased in all three concentration groups at 3–6 hpe, while it decreased at 12 hpe in the 0.68 μM TD49 treatment group. Notably, total protein content increased with TD49 treatment at each time interval. The results revealed that variable effects on the expression of immune-related genes were observed after treatment with TD49. The findings demonstrate that exposure of scallops to TD49 changes immune responses and expression of immune-related genes. We hypothesize that TD49 may disrupt immune system in bay scallop. The current investigation highlights the potential negative effects of using TD49 as an algaecide on marine economic bivalves to control harmful algal blooms in marine environments.

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Section: Discussionmentioning

confidence: 71%

Effect of the Algicide Thiazolidinedione 49 on Immune Responses of Bay Scallop Argopecten Irradians

Chi

Yun²,

Giri³

et al. 2019

Molecules

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“…Antioxidant status in fish, as determined by several antioxidant enzymes including CAT, SOD, GPx, and MDA have been considered as the first line of defensive biomarkers to protect cells and tissues from oxidative damage, caused by some free radicals such as superoxide anion (O 2-), hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (OH) [80,81]. Glutathione peroxidase, GPx is an important antioxidant enzyme showing strong radical-scavenging capacity against free radicals and lipid peroxides [82].…”

Section: Plos Onementioning

confidence: 99%

Total replacement of fishmeal with poultry by-product meal affected the growth, muscle quality, histological structure, antioxidant capacity and immune response of juvenile barramundi, Lates calcarifer

2020

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The present study investigates if the total replacement of dietary fishmeal (FM) with poultry by-product meal (PBM), supplemented with methionine influences the muscle fatty acids composition, normal gut morphology, histological traits of the liver, muscle, and gill, liver enzymes, immune and antioxidant response, and stress-related gene in juvenile barramundi, Lates calcarifer in relation to growth and feed utilization. Barramundi (3.58±0.01g) were randomly distributed into six 300 L seawater recirculating tanks (25 fish/tank) and fed two formulated isonitrogenous and isolipidic diets for 6 weeks. The control diet had FM as the sole animal protein source, whereas other test diet had only PBM as an animal protein source. Dietary PBM affected the fish performance and feed utilization. Regarding muscle fatty acid profile, total saturated fatty acids and monounsaturated fatty acids elevated while total PUFA particularly n-3 LC-PUFA and EPA decreased in PBM fed fish than control diet fed fish. Liver, muscle, gill, and intestinal histology showed no obvious alteration in control diet fed fish, however, more lipid droplets and hepatic vacuolization in the liver, necrotic myotome in muscle, hyperplasia in secondary lamellae in gill and short and broken folds in the intestine were observed in PBM fed fish. Similar to light microscopy observation of intestinal morphology, the transmission electron microscopy (TEM) analysis revealed shorter and smaller microvilli in fish fed PBM. Histopathological alterations in the liver of PBM fed fish were further associated with the elevated levels of aspartate aminotransferase (AST) and glutamate dehydrogenase (GLDH) and the significant upregulation of stress-related genes, HSP70 and HSP90. Also, a negative influence on lysozyme activity, and antioxidant enzymatic activities were recorded in fish fed PBM. Overall, it can be concluded that a total substitution of FM protein by methionine supplemented PBM negatively influenced the growth performance, liver health, histological traits of different organs, immune and antioxidant response, and expression of stress-related genes in juvenile barramundi.

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“…A diet without ICA was used as the control (CON). The ingredients and proximal composition of the CON diet are the same as those reported previously (Zheng et al, 2019). The 32% fishmeal, 20% soybean meal, 13% peanut meal, 4.5% blood powder, and 2% rapeseed meal served as protein sources, and equal portions of fish oil and soybean oil (2.4%) were included as lipid sources.…”

Section: Diets Crab Management and Sample Collectionmentioning

confidence: 99%

“…Subsequently, a small piece of fresh ovary tissue was collected and fixed in Bouin's solution for ovarian histological analysis, and the remaining ovarian tissue and all the hepatopancreas tissue were quick-frozen in liquid nitrogen and stored at -80 • C for further analysis. Next, 0.5 mL of hemolymph was collected from each crab's second last pair of walking legs using a 1-mL sterile syringe, and mixed with pre-inhaled precooling crab anticoagulant solution at the ratio of 1:1 (Zheng et al, 2019), and immediately centrifuged at 9000 r/min and 4 • C for 20 min. The supernatant was collected and stored at -20 • C for later determination of VTG and gonadotropin releasing hormone (GnRH), FSH, and E 2 contents.…”

Section: Diets Crab Management and Sample Collectionmentioning

confidence: 99%

Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis

Zheng

Liu

et al. 2020

Front. Mar. Sci.

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In Eriocheir sinensis aquaculture, crabs feeding on an artificial diet without steroid hormones available in ice-fresh fish or snails show delayed ovarian maturation and late listing. Icariin (ICA), a safe plant-derived monomer compound, can effectively promote estradiol (E 2) synthesis and ovarian development in mammals, but its effect in crustaceans has not been reported. We investigated the effects of ICA on estrogen synthesis, vitellogenesis, and oocyte development in Chinese mitten crabs fed with one of four diets containing 0, 50, 100, and 200 mg/kg ICA, respectively, for 8 weeks. Results showed that vitellogenin (VTG) contents, gonadosomatic indexes (GSIs), and oocyte volumes in ICA50 and ICA 100 crabs were significantly higher than those in the control. The highest upregulated expression of VTG in hepatopancreases and ovaries was detected in ICA100 crabs. Hemolymph E 2 contents were also significantly increased in ICA100 crabs. Enzyme-linked immunosorbent assay (ELISA) and western blot analysis indicated that the increase in E 2 concentrations was attributable to an elevation in aromatase protein levels in ovaries through the cyclic adenosine monophosphate (cAMP)/protein kinase A (PKA)/cAMP response element binding (CREB) protein pathway. These findings suggest that ICA could serve as a natural additive to resolve the problem of delayed ovarian maturation of crabs raised in ponds.

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Effects of dietary supplementation with icariin on growth performance, antioxidant capacity and non-specific immunity of Chinese mitten crab (Eriocheir sinensis)

Cited by 61 publications

References 67 publications

Effect of the Algicide Thiazolidinedione 49 on Immune Responses of Bay Scallop Argopecten Irradians

Effect of the Algicide Thiazolidinedione 49 on Immune Responses of Bay Scallop Argopecten Irradians

Total replacement of fishmeal with poultry by-product meal affected the growth, muscle quality, histological structure, antioxidant capacity and immune response of juvenile barramundi, Lates calcarifer

Dietary Supplementation With Icariin Affects Estrogen Synthesis, Vitellogenesis, and Oocyte Development in the Chinese Mitten Crab, Eriocheir sinensis

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