Effects of different carbon sources addition on nutrition composition and extracellular enzymes activity of bioflocs, and digestive enzymes activity and growth performance ofLitopenaeus vannameiin zero-exchange culture tanks

Abstract

A 35-day feeding experiment was conducted to investigate the effects of different carbon sources addition on nutritional composition and extracellular enzymes activity of bioflocs, and digestive enzymes activity and growth performance of Litopenaeus vannamei juveniles (average 5.52 AE 0.21 g) in zero-water exchange culture tanks. Molasses, corn flour and wheat bran were used as carbon sources and added into the tanks to promote the development of bioflocs during the experiment. During the entire experiment, good water quality and biofloc development were achieved under the addition of different carbon sources. At the end of the experiment, the proximate composition and extracellular enzymes activities of the collected bioflocs from seven biofloc groups were influenced by the addition of the different carbon sources. Meanwhile, the specific activities of protease, amylase, lipase and cellulase in the hepatopancreas, stomach and intestine of the shrimp showed differences among the seven biofloc groups, and most of them were significantly higher than those obtained in the control group (P < 0.05). There were differences in the performance (growth and FCR) of the shrimp among the seven biofloc groups, and all of them were significantly better than those obtained in the control group (P < 0.05). Based on the results of this study, 60% molasses + 20% corn flour + 20% wheat bran could be an appropriate formula of the addition of carbon sources for intensive culture of L. vannamei (mainly in terms of growth and FCR) in zero-water exchange culture tanks.

Effect of the C/N ratio on inorganic nitrogen control and the growth and physiological parameters of tilapiasfingerlings,Oreochromis niloticureared in biofloc systems

“…The formation and development of biofloc in the water column of an aquaculture tank is likely linked to the direct assimilation of dissolved nitrogenous matter (especially ammonia nitrogen) from feed and fish excretions by heterotrophic bacteria (Schneider, Sereti, Eding, & Verreth, ; Wang et al., ); thus, levels of TAN can be controlled within recommended ranges for fish culture. In this study, no water was discharged from the biofloc tanks during the 120‐day trial period, and the three forms of inorganic nitrogen remained in the safe range for tilapia aquaculture (Luo et al., ).…”

Effects of Different Carbon Sources on Bioactive Compound Production of Biofloc, Immune Response, Antioxidant Level, and Growth Performance of Litopenaeus vannamei in Zero‐water Exchange Culture Tanks

“…It was possible that wheat bran, a kind of high fiber and slightly soluble substance, was poorly utilized by biofloc. The results of this study concurred with the study of Wang et al () in which adding varied levels of molasses, corn flour, and wheat bran into L . vannamei culture system could result in gradual increases of both BFV and TSS levels.…”

“…It was possible that wheat bran, a kind of high fiber and slightly soluble substance, was poorly utilized by biofloc. The results of this study concurred with the study of Wang et al (2015) in which adding varied levels of molasses, corn flour, and wheat bran into L. vannamei culture system could result in gradual increases of both BFV and TSS levels. Some studies reported that regulating C/N ratio or adding different carbon sources could affect T-AOC = total antioxidant capacity; SOD = superoxide dismutase; GSH = reduced glutathione; GSSG = oxidized glutathione; control = without addition of molasses and wheat bran; T1 = addition of 100% molasses; T2 = addition of 50% molasses and 50% wheat bran; T3 = addition of 75% molasses and 25% wheat bran.…”

JuvenileProcambarus clarkiifarmed using biofloc technology or commercial feed in zero‐water exchange indoor tanks: A comparison of growth performance, enzyme activity and proximate composition

“…Biofloc technology has been applied to the farming of a variety of crustaceans, including Litopenaeus vannamei (Wang et al, ), Macrobrachium rosenbergii (Asaduzzaman et al, ), Penaeus monodon (Ahmad et al, ) and Artemia franciscana (Crab, Lambert, Defoirdt, Bossier, & Verstraete, ). However, there is currently only one study (Li, Cheng, Wang, Wang, Chen & Li, ) on the use of biofloc technology for the cultivation of crayfish.…”