Effects of different cold atmospheric‐pressure plasma sources on the yeast Candida glabrata

Abstract

Four different cold plasma sources were directly applied onto a 24h inoculum of Candida glabrata inoculated on agar plates, within the limits of in vitro experiment. Their effects were compared and evaluated with respect to the size and stability of the inhibition zones formed in the posttreatment cultivation. The results prove significant inhibitory cold atmospheric‐pressure plasma effects on the yeast C. glabrata. The overall inhibitory effects are directly proportional to the treatment time, the applied power, and the overall functioning of the plasma source and indirectly proportional to the initial cell concentration, although this factor was less significant compared to the other examined factors. The unipolar microwave torch was found to be the most effective in the inhibition of C. glabrata.

Plasma-Activated Media Produced by a Microwave-Excited Atmospheric Pressure Plasma Jet Is Effective against Cisplatin-Resistant Human Bladder Cancer Cells In Vitro

“…The DW's electrical conductivity and pH were measured using a conductivity stick meter (Starter ST3100C) and a digital pH meter (Starter ST3100) both Ohaus Corp., Parsippany, NJ, USA. Although the concentration of the active species is the highest at the tip of the plasma plume [60], the treated liquid surface was placed at 5 mm below the plume tip to prevent severe evaporation. The T-24, HT29, and DLD-1 cells were grown in DMEM (Capricorn Scientific GmbH, Ebsdorfergrund, Germany) and the MDA-MB-231, RKO, and HCT116 cells were incubated in Roswell Park Memorial Institute with L-glutamine media (Capricorn Scientific GmbH, Ebsdorfergrund, Germany).…”

Interdisciplinarity: In the DNA of plasma medicine

“…The article from Trebulová et al investigates the action of different plasma sources for preventing and treating fungal infections, which could be employed in different approaches (i.e., surface decontamination, medical device sterilization, or direct treatment of infected tissues) depending on their configuration. [ 9 ] From another perspective, Jirásek et al explore the chemistry underlying the antimicrobial effects of plasma‐treated liquids (PTLs). This article emphasizes the significance of comprehending the chemical processes and reactions within PTLs to establish a correlation between the chemical aspects of the PTLs and their therapeutic effects in medical applications.…”