Four different cold plasma sources were directly applied onto a 24h inoculum of Candida glabrata inoculated on agar plates, within the limits of in vitro experiment. Their effects were compared and evaluated with respect to the size and stability of the inhibition zones formed in the posttreatment cultivation. The results prove significant inhibitory cold atmospheric‐pressure plasma effects on the yeast C. glabrata. The overall inhibitory effects are directly proportional to the treatment time, the applied power, and the overall functioning of the plasma source and indirectly proportional to the initial cell concentration, although this factor was less significant compared to the other examined factors. The unipolar microwave torch was found to be the most effective in the inhibition of C. glabrata.

Acne vulgaris is a prevalent skin condition that is caused by an imbalance in skin microbiomes mainly by the overgrowth of strains such as Cutibacterium acnes and Staphylococcus epidermidis which affect both teenagers and adults. Drug resistance, dosing, mood alteration, and other issues hinder traditional therapy. This study aimed to create a novel dissolvable nanofiber patch containing essential oils (EOs) from Lavandula angustifolia and Mentha piperita for acne vulgaris treatment. The EOs were characterized based on antioxidant activity and chemical composition using HPLC and GC/MS analysis. The antimicrobial activity against C. acnes and S. epidermidis was observed by the determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The MICs were in the range of 5.7–9.4 μL/mL, and MBCs 9.4–25.0 μL/mL. The EOs were integrated into gelatin nanofibers by electrospinning and SEM images of the fibers were taken. Only the addition of 20% of pure essential oil led to minor diameter and morphology alteration. The agar diffusion tests were performed. Pure and diluted Eos in almond oil exhibited a strong antibacterial effect on C. acnes and S. epidermidis. After incorporation into nanofibers, we were able to focus the antimicrobial effect only on the spot of application with no effect on the surrounding microorganisms. Lastly, for cytotoxicity evaluation, and MTT assay was performed with promising results that samples in the tested range had a low impact on HaCaT cell line viability. In conclusion, our gelatin nanofibers containing EOs are suitable for further investigation as prospective antimicrobial patches for acne vulgaris local treatment.