Effects of Different Concentrations of Lactobacillus plantarum and Bacillus licheniformis on Silage Quality, In Vitro Fermentation and Microbial Community of Hybrid Pennisetum

Zhu, Yanchen; Xiong, Haoming; Tian, Hanchen; Chen, Yiye; Wu, Long‐Fei; Guo, Yongqing; Sun, Baoli

doi:10.3390/ani12141752

Cited by 20 publications

(15 citation statements)

References 50 publications

(44 reference statements)

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“…A study demonstrated that addition of Lactiplantibacillus plantarum and cellulase decreased the abundance of Weissella and Leuconostoc [52]. Another study indicated that adding both 1 × 10 5 and 1 × 10 7 cfu/g of L. plantarum could reduce the relative abundance of Weissella in silage [ 53 ] , and the optimum addition amount of Lactiplantibacillus plantarum is 1 × 10 7 cfu/g FW, which was consistent with our results. In this study, the addition of L. plantarum also significantly decreased the relative abundance of Weissella compared with that of the control group.…”

Section: Effects Of Harvest Period and Lactiplantibacillus Plantarum ...supporting

confidence: 90%

Effects of Lactiplantibacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages

Dong

Chen

et al. 2022

Chem. Biol. Technol. Agric.

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Background Silage is achieved by anaerobic fermentation of lactic acid bacteria. However, harvest stage and Lactiplantibacillus plantarum (L. plantarum) content affect the changes of microbial community in silage, which play an important role in silage quality and mycotoxin levels. The current study evaluated the effects exerted by the application of L. plantarum on fermentation, mycotoxins and bacterial community of whole-crop corn silage at different harvest stages. The fresh whole plant corn from the same area was harvested during the milk stage and the dough stage, following which from each harvest stage was subjected to four L. plantarum treatments: 0 cfu/g; 1 × 106 cfu/g; 1 × 107 cfu/g; and 1 × 108 cfu/g in a 2 × 4 factorial experimental design. Samples were collected on days 0, 3, 7, 21 and 42. Subsequently, nutrition parameters, fermentation parameters, mycotoxin levels and bacterial community were determined. Results Prolonging the harvest stage resulted in an increase in the levels of starch and dry matter as well as deoxynivalenol in corn silage. Addition of 1 × 107 cfu/g and 1 × 108 cfu/g of L. plantarum applied after 42 d of ensilage reduced dry matter loss, which significantly lowered deoxynivalenol concentration in corn silage at the dough stage (P < 0.01). The pH of corn silage decreased, whereas the lactic acid concentration increased after ensiling. The effects exerted by the interaction (harvest stage × dose of L. plantarum) on pH on day 21 as well as on fermentation and lactic acid on days 21 and 42 were significant (P < 0.05). Addition of 1 × 107 cfu/g of L. plantarum at the dough stage significantly increased the relative abundance of Firmicutes while decreasing that of Bacteroidetes, compared to when L. plantarum was not added (P < 0.05). Conclusions Adding L. plantarum improved the fermentation quality and bacterial community of silage, which reduced part of mycotoxin content and relative abundance of harmful bacteria. In addition, adding L. plantarum in dough stage resulted in greater improvements to mycotoxin content and bacterial community.

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Section: Effects Of Harvest Period and Lactiplantibacillus Plantarum ...supporting

confidence: 90%

Effects of Lactiplantibacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages

Dong

Chen

et al. 2022

Chem. Biol. Technol. Agric.

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“…Inoculation with Bacillus subtilis has been shown to lower pH and increase lactic acid concentrations in maize silage [62,63]. When used as silage inoculants, Bacillus licheniformis [64] and Bacillus subtilis [65] have been proven to be effective at reducing yeast and mold quantities and increasing fiber degradation [64]. Using Bacillus as an inoculant has been shown to increase the viable count of LAB in maize silage [65]; however, we did not observe this in our study.…”

Section: Effects Of B Cereus On Silage Qualitycontrasting

confidence: 78%

Fermentation and Microbial Community of Maize Silage Inoculated with Lentilactobacillus buchneri NCIMB 40788 and Contaminated with Bacillus and Clostridium Spore Formers

Huffman,

Drouin,

Dunière

et al. 2023

Fermentation

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Spore-forming bacteria in silage may reduce dry matter intake or affect dairy product quality when transferred to milk. The present study investigated the effects of three facultative anaerobes (Bacillus cereus, Bacillus subtilis, and Bacillus licheniformis) and two strict anaerobes (Clostridium tyrobutyricum and Clostridium beijerinckii) commonly found in low-quality silage, milk, and cheese. Maize silage was intentionally contaminated with these spore formers in separate mini silos at 1 × 105 CFU spore former per g and treated with commercial silage inoculant Lentilactobacillus buchneri NCIMB 40788 at 4 × 105 CFU per g or left untreated. The microbial and chemical profiles of maize silage, which were determined using Nuclear Magnetic Resonance (NMR), were measured after fermentation for 100 days, and they were also measured for silage exposed to air for 72 h after opening at 100 days. The effect of the inoculant strain L. buchneri NCIMB 40788 on these contaminated silages was investigated to determine if the inoculant could prevent/limit the negative impacts caused by the spore former contaminants. Overall, inoculation improved silage quality and aerobic stability. Acetic acid content was higher in the INOC samples than in the NIS (p < 0.001), while lactic acid content was lower in INOC than in NIS (p < 0.001). Both lactic and acetic acid levels were higher in the silage samples contaminated with B. cereus. Contamination with the spore formers increased the aerobic and anaerobic spore counts of the samples contaminated with B. subtilis and B. licheniformis compared to the control silage after opening. After 3 days of aerobic exposure, the samples contaminated with B. cereus, B. subtilis, and B. licheniformis showed higher aerobic spore counts than the control. The dominant bacterial population was significantly modified by inoculation. Neither inoculation nor contamination types impacted fungal populations upon opening, but a dominance of Wickerhamomyces was observed after aerobic exposure in the B. cereus silages. The γ-aminobutyrate (GABA) content after aerobic exposure was higher than the uncontaminated control for the silage contaminated with B. licheniformis. The samples contaminated with Clostridium species remained largely unchanged compared to the control samples. Physicochemical and bacterial profiles were mainly affected by inoculation, and a limited impact of spore contaminant was noted. Our results show that when L. buchneri inoculation was carried out, higher aerobic and anaerobic spore counts following contamination with bacterial spore formers were mitigated after reaching aerobic stability.

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“…Ensiling is a good method for preserving fresh forages in anaerobic conditions (Weinberg et al 2010 ). Different additives have been used to increase the speed of pH reduction after ensiling, improve silage fermentation and increase the digestibility of fodders (Muck et al 2018 ; Kazemi et al 2019 , 2022 ; Zhu et al 2022 ). Based on Contreras et al ( 2020 ) reports, the addition of SC to barley silage (5, 10, and 15 g/kg fresh matter) had a detrimental effect on the nutritional quality of barley after ensiling.…”

Section: Introductionmentioning

confidence: 99%

Can Alhaji maurorum as a halophyte plant be ensiled with molasses and Saccharomyces cerevisiae well?

Kazemi

Valizadeh

2023

AMB Expr

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Alhagi maurorum (Caspian Manna, AM) is a species of legume found commonly in the semi-arid region of the world. Nutritional aspects of silage prepared from AM have not been scientifically investigated so far, therefore, in this study, chemical-mineral composition, gas production parameters, ruminal fermentation parameters, buffering capacity, and silage characteristics of AM were investigated by standard laboratory methods. Fresh AM was ensiled in the mini-silos (3.5 kg) and treated with (1) no additive (control), (2) 5% molasses, (3) 10% molasses, (4) 1 × 104 CFU of Saccharomyces cerevisiae [SC]/g of fresh silage, (5) 1 × 104 CFU of SC/g of fresh silage + 5% molasses, (6) 1 × 104 CFU of SC/g of fresh silage + 10% molasses, (7) 1 × 108 CFU of SC/g of fresh silage, (8) 1 × 108 CFU of SC/g of fresh silage + 5% molasses, and (9) 1 × 108 CFU of SC/g of fresh silage + 10% molasses for 60 days. The lowest concentrations of NDF and ADF were related to treatments no. 6 and 5, respectively (p < 0.0001). The ash content as well as sodium, calcium, potassium, phosphorus and magnesium were highest in treatment no 2. Silages containing 10% molasses (no. 3) had the highest and lowest lactic (p < 0.0001) and butyric (p < 0.0001) acids, respectively. The highest amount of potential gas production was observed in treatments no. 5 and 6, respectively (p < 0.0001). Total yeast was decreased with increasing molasses in the silages (p < 0.0001). Acid-base buffering capacity was also highest in treatments no. 6 and 5, respectively (p = 0.0003). In general, due to the fibrous nature of AM, it is recommended to add molasses at levels of 5 or 10% when ensiling. The silages containing SC at a lower level (1 × 104 CFU) along with higher levels of molasses (10% of DM) had better ruminal digestion-fermentation characteristics compared to other silages. Also, the addition of molasses improved the internal fermentation characteristics of AM in the silo.

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Effects of Different Concentrations of Lactobacillus plantarum and Bacillus licheniformis on Silage Quality, In Vitro Fermentation and Microbial Community of Hybrid Pennisetum

Cited by 20 publications

References 50 publications

Effects of Lactiplantibacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages

Effects of Lactiplantibacillus plantarum inoculation on the quality and bacterial community of whole-crop corn silage at different harvest stages

Fermentation and Microbial Community of Maize Silage Inoculated with Lentilactobacillus buchneri NCIMB 40788 and Contaminated with Bacillus and Clostridium Spore Formers

Can Alhaji maurorum as a halophyte plant be ensiled with molasses and Saccharomyces cerevisiae well?

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