Effects of different dietary lipid sources on tissue fatty acid composition, serum biochemical parameters and fatty acid synthase of juvenile mud crabScylla paramamosain(Estampador 1949)

Abstract

A 6‐week study was conducted to determine the effects of different lipid sources in pelleted diets on juvenile mud crab Scylla paramamosain. Five isonitrogenous and isolipidic diets containing 8% level of fish oil (FO), lard (LD), safflower oil (SO), perilla seed oil (PO) or mixture oil (MO; VFO:VSO:VPO = 1:1:1), and a live food of marine bivalve Potamocorbula rubromuscula as the control diet (CF), were fed to groups of 25 juvenile crabs (average initial weight 7.4 g, carapace width 3.5 cm) in triplicate. The results showed that crabs fed MO had the highest survival (P < 0.05). The moisture content was significantly higher in crabs fed LD, SO and PO (P < 0.05). Crabs fed SO exhibited the lowest crude protein and lipid (P < 0.05). Ash contents were obviously lower in LD group (P < 0.05). Highest total lipid in the hepatopancreas and muscle was in LD and FO group respectively. Glucose, total cholesterol and low‐density lipoprotein were higher while high‐density lipoprotein was lower (P < 0.05) in LD group. Tissue fatty acid compositions were consistent with those in diets. FO and MO diets had the same depression effect like CF on fatty acid synthase activity and mRNA expression in the hepatopancreas. The results of this study indicated that FO and mixed oil are suitable for preparation of pelleted diets with better effects for juvenile S. paramamosain compared with live food, and the ratio of n‐6/n‐3 fatty acids in pelleted diets must be <1.

Effects of Dietary Soybean Oil Replacement by Silkworm,Bombyx moriL., Chrysalis Oil on Growth Performance, Tissue Fatty Acid Composition, and Health Status of Juvenile Jian Carp,Cyprinus carpiovar. Jian

“…However, our results showed that the serum GLU of Jian carp was not influenced by the experimental diets. Zhao et al () reported no significant differences in the serum Chol and TG levels of mud crabs between the safflower oil group (rich in LA) and the perilla seed oil group (rich in LNA), corresponding to our results.…”

Effects of dietary lipid sources on growth performance, fatty acid composition and hepatic lipid metabolism of juvenile blunt snout bream, Megalobrama amblycephala

“…We show that blunt snout bream fed the CO diet had the highest levels of FAS and ACC activities and the highest FAS expression, indicating that high levels of dietary SFA increase the activity and mRNA expression of FAS. Similarly, Zhao, Wen, Li, Zhu, and Li () reported that compared to saturated fatty acids, polyunsaturated fatty acids have a stronger inhibitory effect on FAS activity. CPT I and CPT II are considered to be the key regulatory enzymes in mitochondrial β‐oxidation (Kerner & Hoppel, ).…”

Dietary lipid and n-3 long-chain PUFA levels impact growth performance and lipid metabolism of juvenile mud crab, Scylla paramamosain

“…In 2018, the yield of farmed mud crabs (mainly S. paramamosain) reached 157 712 tons (19) . However, the development of commercial feed for S. paramamosain has lagged in the crab farming industry, and there are few reports on the nutritional requirements of mud crab (20)(21)(22)(23) , although dietary lipid levels of 8•52-11•63 % (optimum 9•50 %) could maintain growth performance (20) . The requirement for dietary LC-PUFA in swimming crab was supposed to range from 6•0 to 23•6 mg/g of diets (7,8,10,11) , and the optimum dietary DHA: EPA ratio for juvenile crab was about 1•0.…”