Effects of Different Environmental Factors on the Growth and Bioactive Substance Accumulation of Porphyridium purpureum

Abstract

Genus Porphyridium is a primitive single-celled red algae widely distributed in seawater, freshwater, and moist soil. It can synthesize bioactive substances such as phycoerythrin, extracellular polysaccharides and polyunsaturated fatty acids during the growth process. In this paper, the culture and bioactive substance yield of Porphyridium purpureum were studied by setting salinity, nitrogen-to-phosphorus ratio, and pH at different gradient levels. The results showed that the optimal conditions for the growth of P. purpureum were salinity 34 ppt, nitrogen-to-phosphorus ratio 169:1, and pH 8; the optimal conditions for obtaining the polysaccharides were salinity 17 ppt, nitrogen-to-phosphorus ratio 14:1, and pH 8; the optimal conditions for obtaining phycoerythrin were salinity 17 ppt, nitrogen-to-phosphorus ratio 68:1, and pH 8; the optimal conditions for obtaining the lipids were salinity 34 ppt, nitrogen-to-phosphorus ratio 1:1, and pH 8. In actual production applications, culture conditions should be set according to different product accumulation purposes in order to achieve the optimal production efficiency.

Malaysian Virgin Soil Extracts as Natural Growth Enhancer for Targeted Green Microalgae Species

“…A study carried out by Allaguvatova [34] reported enhanced production of Chlorella vulgaris due to the addition of soil extracts and vitamins to the culture medium as evidenced by the increase in the optical density. Similarly, Porphyridium purpureum cultivated in Kock medium in addition to soil extracts promoted the growth of the microalgae as revealed by the research established by Lu [35].…”

Effect of salinity on the biochemical characteristics and antioxidant activity of exopolysaccharide of Porphyridium purpureum FACHB 806

“…For instance, the growth rate of P. cruentum was 3-fold higher at optimal salinity (0.8 mol•L -1 NaCl) compared to 1.5 mol•L -1 NaCl (Lee et al, 1989). P. purpureum grew slowly under excessively low (0 ‰) or high salinity (68 ‰) conditions (Lu et al, 2020). The growth inhibition of microalgae when exposed to salinity stress might be attributed to the loss of water, ionic imbalance, reduction of photosynthesis rate and overproduction of reactive oxygen species (Martıńez-Roldań et al, 2014).…”

“…Porphyridium has strong salinity tolerance and the 35‰ -46‰ salinity had no significant effect on its growth (Li et al, 2019a). Salinity could influence the synthesis of EPS, total lipids and phycoerythrin in Porphyridium (Lu et al, 2020). This study aimed to investigate the effect of salinity on the yield, biochemical characteristics and antioxidant activity of P. purpureum EPS.…”

Therapeutic Potential of Microalgae-Derived Bioactive Metabolites Is Influenced by Different Large-Scale Culture Strategies

“…In contrast, if the goal is to obtain EPS, the culture must be run in batch mode, in order to stress the culture and reach the stationary phase, allowing the EPS accumulation as a response of the nutrient (mainly nitrogen) depletion [ 14 , 30 ]. Different stress strategies in microalgae have been applied in different microalgae strains to obtain several metabolites from protein, carbohydrate, or lipid fractions [ 23 , 31 , 32 , 33 ]. In this study, we evaluated whether stressing (by nutrient depletion) two common commercial marine microalgae cultures resulted in biomass extracts with different bioactivity properties using ovarian SKOV3 cancer cells as a cancer model.…”