Effects of different postbreeding treatments on fertility of thoroughbred mares

Mattos, R.C.; Malschitzky, Eduardo; Mattos, Rodrigo Costa; Gregory, Ricardo Macedo

doi:10.21836/pem19970514

Cited by 6 publications

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“…Data presented in this work showed that the pregnancy rate of maiden mares bred in the 1 st estrus cycle during the breeding season was significantly lower (63.7%) than that of the mares bred in other estrus cycles (80.3%), results similar to those observed previously by Mattos et al (1997 and1999). However, no significant difference (p>0.05) regarding the EL rate was observed between the first and other estrus cycles, being the EL rates in agreement with those found by Ginther (1992).…”

Section: Discussionsupporting

confidence: 91%

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Malschitzky¹,

Schilela²,

Mattos³

et al. 2003

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The aim of the present study was to compare pregnancy and embryo loss rates in non-lactating mares bred either in the first, or in other estrus cycles during the breeding season. A total of 324 non-lactating mares, 179 of them barren and 145 maiden, were allocated in a stallion management center in Southern Brazil. All the mares were submitted to an artificial photoperiod of 15 hours light/9 hours darkness, either from May 15th on, or later on, from the moment they entered the management center. All the mares have been examined twice a week, from July 15th to July 31st, in order to determine the estrus phase, being classified, according to the findings, into two groups: group 1 = 1st estrus-mares showing reduced uterine tonus, small sized ovaries or ovaries with irregular follicular activity, lacking evidence of preovulatory follicle and, mainly, of corpus luteum (CL) at ultrasound; group 2 = ∆ 2 nd estrus-mares presenting CL at examination. The last ones received Prostaglandin on the 5th day after CL identification, or later on, for induction of a new estrus cycle. From August 1st on, all mares in estrus have been examined at maximal intervals of 48 hours, in order to evaluate follicular growth, grade of uterine edema and presence of intra-uterine fluid (IUF). Mares have been bred when a preovulatory follicle and low grade of uterine edema were found. A new examination was performed 36 to 48 hours after breeding to confirm ovulation and to detect IUF. Mares showing IUF were treated by means of uterine flushes and antibiotic infusion and those showing IUF for more than three consecutive days were excluded from the experiment. Barren and maiden mares bred in the 1st estrus cycle showed significant lower (p<0,026) pregnancy rates in days 12 and 42 in comparison to mares bred during subsequent cycles. Barren mares bred during the 1st estrus cycle showed higher embryo loss rate (p= 0.048) than barren mares bred during other cycles (20.4% vs. 8.0%). Embryo loss rate was not significantly different in maiden mares bred in 1st or in other estrus (9.1% vs. 4.9%). Grade of uterine edema and presence of IUF during estrus did not differ in barren and maiden mares bred either in the 1st, or in other cycles. However, barren mares bred during the 2nd and other cycles showed higher incidence of IUF (p=0.040) 36 to 48 hours after breeding than those bred in the 1st estrus cycle (51.7% vs.27.5%). Presence of IUF was not significantly different (p=0.680) between maiden mares bred during the 1st or other cycles (18,2% vs. 27.0%). It was concluded that: (a) during the breeding season the fertility of non-lactating mares is lower in the 1st than in other estrus cycles; (b) that a higher embryo loss rate may be expected in barren mares bred in the 1st than in other estrus cycles of the breeding season and (c) that the lower fertility rate observed during the 1st estrus cycle is not related to the incidence of post-breeding endometritis.

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Section: Discussionsupporting

confidence: 91%

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Malschitzky¹,

Schilela²,

Mattos³

et al. 2003

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“…Hiermit ist die In-Vitro-Basis fur eine mögliche therapeutische Anwendung des Verfahrens geschaffen, die in der intrauterinen Inokulation vitaler und im mu nk o mp et e n te r , ho rn o lo g e r od e r h e ter o lo ge r PM N bei bakteriell endometritiserkrankten Stuten bestehen wird. Felduntersuchungen an suszeptiblen StLrten liegen inzwischen vor (Castilho 1994;Mattos et al 1997) . Nach der Bedeckung wurden aus dem Blut frisch separierle PMN intrauterin appliziert.…”

Section: Materials Und Methodenunclassified

Separation and Cryopreservation of lmmune Competent Polymorphonuclear Granulocytes (PMN) for Intrauterine Inoculation in Equine Endometritis

Castilho¹,

Zerbe²,

Rabe³

et al. 1997

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ZusammenfassungAus Sper-rderblut von 6 Stuterr wurden neutrophrle Granulozfen mittels hypotoner Erythrozytenlyse und Zentrrfugation separiert und tiefgefroren in Makrotüb konserviert. Für den Einfriervorgang wurde ein Kryoprotektivum bestehend aus Pferde-Blutplasma und DMSO (5%) sowie e in Pr o tok o l l m it ei n erEin fri ert em pe rat u rku rve( 4b i s-70 "C1 "C/min ; -71 b is -14 0' C1 O"C /min ; -14 1 b i s-l96 "CTa u che ni nfl üssig en St ickstoffl entwickelt. Vitalität, Bildung reaktiver Sauerstoffspezies und Phagozytoseaktivität der aufgetauten PMN wurden mittels durchflußzyto-metrischer Meßmethoden mit frischen, nicht gefrorenen Zellen des identischen Spenderlieres verglichen. lm Vitalitätstest wurden mit 95,506 resp. 94,5o./o membranintakten PMN glejchwertige Resultate bei den Kontrollen resp. gefrorenen/aufgeiauten PMN-Populationen gemessen. Die Phagozytoseaktivität opsonisierter Bakterien war mit 81,0 % resp. 80,0 % in den frischen resp. gefrorenen/aufgetauten PMN-Populationen iderrtisch, Die gefrorenen/aufgetauten PMN zeigten eine signifikant höhere Kapazität zur ROSBildung nach Stimulation mit Pl\/A. r-ria trr^öhnicca Äiocor at;lig demonstrieren die Brauchbarkeit des Verfahrens zur Gewinnung und Gefrierkonservierung einer vitalen und immunkompetenten PMN-Population für den geplanten therapeutischen Einsatz bei bakteriell bedingter equiner Endometritis.Schlüsselwörter: Stute, Endometritis, neutrophile Granulozyten, Kryokonservierung Separation and Cryopreservation of lmmune Competent Polymorphonuclear Granulocytes (PMN) for Intrauterine Inoculation in Equine EndometritisPMN from blood of 6 mares were separated using hypotonic lysis of erythroc\4es and centrifugation, After addition of a solution of equine plasma and DMSO (5%) the PMN were frozen. A method for freezing was developed with control of temperature decreasing rate. Tempera ture curve was 4 to -70"C at 1'Clmin, -71 to -140"C at 1O"C/min, 140 to -196"C diving. To verify cell survival rate after thawing as well as its functional capacity i.e. phagocytosis and respiratory burst (ROS) an experiment using flow cytometry assay was pedormed. 95.5 o/o resp. 94.5 o/o of propidium iodide negative PMNs indicate cell membrane integrity of both the fresh and frozenlthawed PMN populations. Phagocytosis of opsonized bacteria was similar in both populations of cells i.e. 81.0 % resp. 80.0 % of the tresh resp. frozenlthawed PMNs. Increasing capacity for generation of reactive oxygen species after stimulation by PMA was obserued with frozenlthawed neutrophils. The results of this study indicate the practicability of the method for achieving and cryopreservation of a vital and immune competent PMN oooulation for intended theraoeutic use in susceptible mares for endometritis.

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Reproductive Tract Infections

Tibary¹,

Fite²

2007

Equine Infectious Diseases

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Effects of different postbreeding treatments on fertility of thoroughbred mares

Cited by 6 publications

References 2 publications

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Pregnancy and embryo loss rates in non-lactating mares bred in the first or in other estrus cycles during the breeding season

Separation and Cryopreservation of lmmune Competent Polymorphonuclear Granulocytes (PMN) for Intrauterine Inoculation in Equine Endometritis

Reproductive Tract Infections

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