Effects of Different Rootstocks in Micrografting on Growing of Washington Navel Orange Plants Obtained by Shoot Tip Grafting

Sertkaya, G.

doi:10.1080/13102818.2004.10817091

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…'Carrizo' citrange seeds were germinated in darkness for up to six weeks until used as a rootstock for micrografting. To enhance the scion growth in in vitro micrografting of 'Washington Navel' orange on 'Troyer' citrange seedlings, micrografting scions with two leaves were excised six weeks after micrografting and regrafted on 9-to 10-month-old seedlings of seven Citrus species, and the results suggested sour orange seedlings should be selected for rapid growth of scions obtained by micrografting (Sertkaya, 2004). In almost all of these in vitro micrografting studies, approximately 2-week-old etiolated seedlings of rootstock cultivars such as trifoliate orange, citranges and rough lemon were often used in initial in vitro micrografting and regrafted on other older vigorous seedlings 4-6 weeks after micrografting to enhance growth (Chand et al, 2013;Juarez et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Seedlings from 11 Citrus Accessions for In Vivo Micrografting

Sakai

Mizunoe

et al. 2020

Hortic J

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The very young seedlings of trifoliate orange, and its hybrids with trifoliate leaves as a marker, are usually used as a rootstock for in vitro and in vivo micrografting of citrus to eliminate viruses in spring in temperate regions. In tropical and subtropical regions or in summer, however, the production and use of trifoliate orange seedlings is difficult. Therefore, it is necessary to establish suitable micrografting using efficient Citrus seedlings adapted to these regions and summer. In decapitated seedlings for micrografting, adventitious shoots on the cut end of the epicotyls and shoots from cotyledon axillary buds are often formed. Hence, the potential for adventitious shoot formation and cotyledon axillary shoot formation was firstly studied with decapitated seedlings from 11 Citrus accessions and one Poncirus accession as a control. Mature seeds of the 12 accessions were germinated in vivo and seedlings of various ages (2-, 4-, and 8-week-old, 4-month-old and 8-month-old after germination) were decapitated at three (lower, middle, and upper) positions on the epicotyls. Adventitious shoot formation decreased with increases in the age of seedlings decapitated at eight weeks or 4 months after germination. The percentage of decapitated seedlings forming adventitious shoots was different in different accessions ranging from 0% to 100%, and increased with increases in decapitation height in the epicotyls. It was estimated from these results that 2-week-old seedlings of Natsudaidai, Shiikuwasha, 'Hiradobuntan', 'Variegated Daidai', and trifoliate orange 'Flying Dragon' had higher potential to support the initial growth of adventitious shoots, and cotyledon axillary shoots than the others, and that decapitation at the upper one-third and middle of epicotyls resulted in higher adventitious shoot formation than the lower one-third. In in vivo micrografting of satsuma mandarin on the seedlings of these accessions, Natsudaidai, 'Hirado-buntan', and 'Variegated Daidai' seedlings resulted in high micrografting success rates, whereas 'Kabusu', 'Flying Dragon' and Shiikuwasha seedlings resulted in very low success rates. The success rate decreased with increases in seedling age. It has become clear from these results that there is no relationship between the potential for shoot formation and micrografting success and that the 2-week-old seedlings of Natsudaidai, 'Hirado-buntan' and 'Variegated Daidai' are efficient rootstocks for micrografting.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Seedlings from 11 Citrus Accessions for In Vivo Micrografting

Sakai

Mizunoe

et al. 2020

Hortic J

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“…Various culture conditions have been tested to optimize the regrowth of micrografts with success dependent on the plant species and the source of plant material used. In rootstock seedlings, seeds are usually germinated under continuous darkness for 1 to 6 weeks [40,64,85,86], but successful protocols using seedlings germinated in light conditions have also been reported [27,51,72,87]. Working on grapefruit micrografted onto seedling sour orange, Ali et al [40] showed that the grafting success was related to the light conditions during seedling development.…”

Section: Culture Conditionsmentioning

confidence: 99%

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

et al. 2022

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In vitro micrografting is an important technique supporting the micropropagation of a range of plant species, particularly woody plant species. Over the past several decades, in vitro micrografting has become a strategy to facilitate shoot recovery and acclimatization of in vitro-grown horticultural species. This review focuses on studies on horticultural crops over the past two decades that cover the establishment of in vitro micrografting, discusses factors affecting the success of in vitro micrografting, and provides commentary on the contribution of micrografting applications to the field of micropropagation. Considering the important roles of micrografting in the restoration of vigor and rooting competence, in promotion of shoot recovery following somatic embryogenesis and organogenesis, and in facilitation of shoot regrowth after cryopreservation, the potential use of this technique in facilitation of genetic engineering and safe conservation of horticultural species are specially highlighted.

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“…Disease development is slow; it may take several years to manifest symptoms, which include bark scaling of the trunk, and chlorotic flecks and spots on young leaves. Gum may accumulate below the bark scales and may impregnate the xylem producing wood staining and vessel occlusion; these symptoms have been used for field diagnosis of Psorosis (Sertkaya, 2014). Citrus psorosis virus (CPsV), the type species of the genus Ophiovirus, is the putative causal agent of psorosis (Garcia et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Polyclonal antibodies against the recombinantly expressed coat protein of the Citrus psorosis virus

Salem

Arif

Salama

et al. 2018

Saudi Journal of Biological Sciences

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Psorosis is a damaging disease of citrus that is widespread in many parts of the world. Citrus psorosis virus (CPsV), the type species of the genus Ophiovirus, is the putative causal agent of psorosis. Detection of CPsV by laboratory methods, serology in particular is a primary requirement for large-scale surveys but their production has been impaired by the difficulty of obtaining sufficient clean antigen for immunization. Specific PAbs against coat protein were produced in using recombinant DNA approach. The full length CP gene fragment was amplified by RT-PCR using total RNA extracted from CPsV infected citrus leaves and CP specific primers. The obtained product (1320bp) was cloned, sequenced and sub-cloned into p expression vector. Expression was induced and screened in different bacterial clones by the presence of the expressed protein (48kDa) and optimized in one clone. Expressed CP was purified using batch chromatography under denaturing conditions. Specificity of expressed protein was demonstrated by ELISA before used as antigen for raising PAbs in mice. Specificity of the raised PAbs to CPsV was verified by ELISA and western blotting. The raised PAbs were showed highly effectiveness in screening by ELISA comparing with the commercial antibodies purchased from Agritest, Valanzano, Italy. The expression of CPsV CP gene in , production of PAbs using recombinant protein as an antigen, the suitability of these antibodies for use in immunodiagnostics against the CPsV Egyptian isolate have been accomplished in this work.

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Effects of Different Rootstocks in Micrografting on Growing of Washington Navel Orange Plants Obtained by Shoot Tip Grafting

Cited by 3 publications

References 5 publications

Evaluation of Seedlings from 11 <i>Citrus</i> Accessions for <i>In Vivo</i> Micrografting

Evaluation of Seedlings from 11 <i>Citrus</i> Accessions for <i>In Vivo</i> Micrografting

In Vitro Micrografting of Horticultural Plants: Method Development and the Use for Micropropagation

Polyclonal antibodies against the recombinantly expressed coat protein of the Citrus psorosis virus

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