Evaluation of Seedlings from 11 <i>Citrus</i> Accessions for <i>In Vivo</i> Micrografting

Abstract

The very young seedlings of trifoliate orange, and its hybrids with trifoliate leaves as a marker, are usually used as a rootstock for in vitro and in vivo micrografting of citrus to eliminate viruses in spring in temperate regions. In tropical and subtropical regions or in summer, however, the production and use of trifoliate orange seedlings is difficult. Therefore, it is necessary to establish suitable micrografting using efficient Citrus seedlings adapted to these regions and summer. In decapitated seedlings for micrografting, adventitious shoots on the cut end of the epicotyls and shoots from cotyledon axillary buds are often formed. Hence, the potential for adventitious shoot formation and cotyledon axillary shoot formation was firstly studied with decapitated seedlings from 11 Citrus accessions and one Poncirus accession as a control. Mature seeds of the 12 accessions were germinated in vivo and seedlings of various ages (2-, 4-, and 8-week-old, 4-month-old and 8-month-old after germination) were decapitated at three (lower, middle, and upper) positions on the epicotyls. Adventitious shoot formation decreased with increases in the age of seedlings decapitated at eight weeks or 4 months after germination. The percentage of decapitated seedlings forming adventitious shoots was different in different accessions ranging from 0% to 100%, and increased with increases in decapitation height in the epicotyls. It was estimated from these results that 2-week-old seedlings of Natsudaidai, Shiikuwasha, 'Hiradobuntan', 'Variegated Daidai', and trifoliate orange 'Flying Dragon' had higher potential to support the initial growth of adventitious shoots, and cotyledon axillary shoots than the others, and that decapitation at the upper one-third and middle of epicotyls resulted in higher adventitious shoot formation than the lower one-third. In in vivo micrografting of satsuma mandarin on the seedlings of these accessions, Natsudaidai, 'Hirado-buntan', and 'Variegated Daidai' seedlings resulted in high micrografting success rates, whereas 'Kabusu', 'Flying Dragon' and Shiikuwasha seedlings resulted in very low success rates. The success rate decreased with increases in seedling age. It has become clear from these results that there is no relationship between the potential for shoot formation and micrografting success and that the 2-week-old seedlings of Natsudaidai, 'Hirado-buntan' and 'Variegated Daidai' are efficient rootstocks for micrografting.

Elimination of Citrus Tristeza Virus from Triploid Pummelo Cultivar [Citrus maxima (Burm.) Merr.] by In Vivo Micrografting on Seedlings of Three Citrus Cultivars

“…The numbers of days to initiate the growth of scion in summer micrografting are less than those in autumn (Table 2). The similar results have been reported in micrografting of satsuma mandarin (Le et al, 2020). These results suggest that the buds during non-dormancy period result in the acceleration of grafting process and scion growth.…”

“…; 2n=2x=18], polyembryonic 'Natsudaidai' tangelo (C. natsudaidai Hayata) and polyembryonic 'Variegated Daidai' sour orange (C. aurantium Linn. ), whose seedlings showed high potential for adventitious bud formation on the decapitated epicotyls and the high successful in vivo micrografting rates for satsuma mandarin scions (Le et al, 2020), were used for the production of 2week-old rootstock seedlings in this study.…”

Vi ghép bưởi năm roi (Citrus grandis cv. ‘nam roi’) trong điều kiện in vivo

“…Trong khi đó, vi ghép có thể thực hiện trong điều kiện in vivo (George et al, 2008) và kỹ thuật này có thể khắc phục các trở ngại trên. Le et al (2020) đã chọn lọc được một số gốc ghép và vi ghép thành công trên cây quýt (Citrus. unshiu Marc.)…”

<i>In Vivo</i> Micrografting to Eliminate Passiflora Latent Virus from Infected Passion Fruit Plants

“…In vivo micrografting has certain advantages over in vitro techniques; for example, it requires no aseptic handling resulting in a simpler procedure and avoids transplant shock when transplanting from sterile in vitro to in vivo conditions. It is therefore widely used to eliminate citrus viruses in Japan (Ohta et al, 2011;Le et al, 2020), and was also adopted by NARO (National Agriculture and Food Research Organization, Japan) to eliminate viruses and viroids from clean citrus plants prior to certification and distribution as new cultivars (Ohta, 2016).…”