Effects of dihydromyricetin on ARPE‐19 cell migration through regulating matrix metalloproteinase‐2 expression

Wang, Kai; Yang, Shun‐Fa; Hsieh, Yi‐Hsien; Chang, Yuan Yen; Yu, Nuo-Yi; Lin, Hui-Wen; Lin, Hung‐Yu

doi:10.1002/tox.22637

Cited by 9 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cancer cells secrete numerous proteases, including MMPs, urokinase plasminogen, and cathepsin, to degrade the basement membrane and extracellular matrix, thus facilitating the passage of cancer cells into the circulation to metastasize to distant organs [26]. Furthermore, MMP-2 is associated with metastasis in oral cancer, nasopharyngeal carcinoma, eye cancer, and brain cancer [23,[27][28][29]. Therefore, we expected that MMP-2 inhibition would suppress metastasis.…”

Section: Discussionmentioning

confidence: 99%

Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2

Chen

Lin

et al. 2020

Molecules

View full text Add to dashboard Cite

Background: Sesamin is a lignin present in sesame oil from the bark of Zanthoxylum spp. Sesamin reportedly has anticarcinogenic potential and exerts anti-inflammatory effects on several tumors. Hypothesis/Purpose: However, the effect of sesamin on metastatic progression in human head and neck squamous carcinoma (HNSCC) remains unknown in vitro and in vivo; hence, we investigated the effect of sesamin on HNSCC cells in vitro. Methods and Results: Sesamin-treated human oral cancer cell lines FaDu, HSC-3, and Ca9-22 were subjected to a wound-healing assay. Furthermore, Western blotting was performed to assess the effect of sesamin on the expression levels of matrix metalloproteinase (MMP)-2 and proteins of the MAPK signaling pathway, including p-ERK1/2, P-p38, and p-JNK1/2. In addition, we investigated the association between MMP-2 expression and the MAPK pathway in sesamin-treated oral cancer cells. Sesamin inhibited cell migration and invasion in FaDu, Ca9-22, and HSC-3 cells and suppressed MMP-2 at noncytotoxic concentrations (0 to 40 μM). Furthermore, sesamin significantly reduced p38 MAPK and JNK phosphorylation in a dose-dependent manner in FaDu and HSC-3 cells. Conclusions: These results indicate that sesamin suppresses the migration and invasion of HNSCC cells by regulating MMP-2 and is thus a potential antimetastatic agent for treating HNSCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2

Chen

Lin

et al. 2020

Molecules

View full text Add to dashboard Cite

show abstract

“…ARPE‐19 cells were adjusted to 1 × 10 5 cells/well in the presence of kaempferol (0, 25, 50, 75, and 100 μM) for 24 hours. The cell migration and invasion assay was performed as described by Lin et al The invaded cells were photographed using a light microscope and the data are expressed as the average cell number in 4 fields as previously described …”

Section: Methodsmentioning

confidence: 99%

Kaempferol suppresses cell migration through the activation of the ERK signaling pathways in ARPE‐19 cells

Chien

Wang

Chang

et al. 2018

Environmental Toxicology

Self Cite

View full text Add to dashboard Cite

Kaempferol is a flavonoid with anticancer and anti-metastasis activity in different cancer-cell lines. However, the underlying mechanisms by which kaempferol acts on human retinal pigment epithelial (ARPE-19) cells remain unclear. In this study, we demonstrated that kaempferol inhibited migration and invasion in ARPE-19 cells at non-toxic dosages. We discovered that kaempferol obviously reduced the enzyme activity and protein expression of matrix metalloproteinase-2 by increasing the phosphorylated levels of extracellular signal-regulated kinases 1/2 (ERK1/2) signaling pathways. Additionally, ERK1/2-specific inhibitor PD98059 significantly reversed kaempferol's inhibitory effects on migration and expression of MMP-2 in ARPE-19 cells. Overall, our results are the first to demonstrate that kaempferol is capable of inhibiting cell migration by targeting ERK1/2 signaling in human retinal pigment epithelial cells. K E Y W O R D S ERK1/2 signaling, kaempferol, migration, MMP-2

show abstract

“…DHM inhibits the invasion and migration of human retinal pigment epithelial cells (ARPE-19) by decreasing the expression of MMP-2( 64 ). By contrast, Zhang et al ( 65 ) reported that DHM significantly inhibited the migration and invasion of SK-Hep-1 and MHCC97L hepatocellular carcinoma cells by decreasing MMP-9 protein expression, while downregulation of MMP-9 protein expression was closely associated with increased PKC-δ protein levels and decreased phosphorylation of p38, ERK1/2 and JNK in SK-Hep-1 and MHCC97L cells.…”

Section: Multiple Molecular and Cellular Mechanisms Of The Antitumour...mentioning

confidence: 99%

Multiple molecular and cellular mechanisms of the antitumour effect of dihydromyricetin (Review)

Xia,

Zhu

2024

Biomed Rep

View full text Add to dashboard Cite

Dihydromyricetin (DHM) is a natural flavonoid compound with multiple antitumour effects, including inhibition of proliferation, promotion of apoptosis, inhibition of invasion and migration, clearance of reactive oxygen species (ROS) and induction of autophagy. For example, DHM can effectively block the progression of the tumour cell cycle and inhibit cell proliferation. In different types of cancer cells, DHM can regulate the PI3K/Akt pathway, mTOR, and NF-κB pathway components, such as p53, and endoplasmic reticulum stress can alter the accumulation of ROS or induce autophagy to promote the apoptosis of tumour cells. In addition, when DHM is used in combination with various known chemotherapy drugs, such as paclitaxel, nedaplatin, doxorubicin, oxaliplatin and vinblastine, it can increase the sensitivity of tumour cells to DHM and increase the therapeutic effect of chemotherapy drugs. In the present review, the multiple molecular and cellular mechanisms underlying the antitumour effect of DHM, as well as its ability to increase the effects of various traditional antitumour drugs were summarized. Through the present review, it is expected by the authors to draw attention to the potential of DHM as an antitumour drug and provide valuable references for the clinical translation of DHM research and the development of related treatment strategies.

show abstract

Effects of dihydromyricetin on ARPE‐19 cell migration through regulating matrix metalloproteinase‐2 expression

Cited by 9 publications

References 38 publications

Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2

Antimetastatic Effects of Sesamin on Human Head and Neck Squamous Cell Carcinoma through Regulation of Matrix Metalloproteinase-2

Kaempferol suppresses cell migration through the activation of the ERK signaling pathways in ARPE‐19 cells

Multiple molecular and cellular mechanisms of the antitumour effect of dihydromyricetin (Review)

Contact Info

Product

Resources

About