Effects of Dosage and Treatment Phase of Two GnRH Analogues at the Estrous Stage on Superovulation in Ewes.

Okada, Aki; Andoh, Toshio; Mizuochi, Yasutomo; Yoshii, Koji; Ishida, Naohisa; Fukui, Yutaka

doi:10.1262/jrd.47.275

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…From April to December, 2000, 79 donor ewes were treated with a vaginal sponge impregnated with synthetic progesterone (FGA sponge: chronogest; intervet, Boxmeer, Holland) for 12 days, followed by single 20 mg FSH and 250 IU eCG injections for estrous synchronization and superovulation [12]. All ewes were inseminated at 32 to 36 h after FGA sponge removal and embryos were recovered surgically by uterine flushing 5 days after insemination.…”

Section: Donor Ewesmentioning

confidence: 99%

Viability of Cryopreserved and Vitrified Embryos and Fertility after Direct Transfer in Ewes.

Okada

Yoshii

Mizuochi

et al. 2002

Journal of Reproduction and Development

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Abstract. The present study investigated the in vitro viability of cryopreserved-thawed ovine embryos by three methods, slow freezing (ethylene glycol (EG)-3 step method), freezing for direct transfer (Direct method) and vitrification (Experiment 1), and the in vivo viability by two transfer methods, direct ET (d-ET) and stepwise ET (s-ET), for the Direct method and vitrified embryos, respectively (Experiment 2). In vivo produced embryos (morula and blastocyst) were recovered from 79 superovulated and artificially inseminated ewes, and were cryopreserved by three methods: EG-3 step method, Direct method and vitrification. In Experiment 1, all thawed embryos were removed from the respective cryoprotectants and cultured for 144 h. There were no significant differences among in the mean survival rates, normal hatching rates (by 72 h) and hatching rates (by 144 h) of the three methods. The viability of frozen-thawed embryos by the EG-3 step method tended to be higher than that of the other methods, but some of the viable embryos frozen by the EG-3 step method had delayed hatching or were degenerated (28.6% vs. 16.7% for Direct method and 6.7% for vitrification). In Experiment 2, the frozen-thawed embryos were transferred by s-ET and d-ET. The pregnancy and lambing rates of both transfer methods for embryos frozen-thawed by the Direct method were 25.0%, but the subsequent viability of embryos in d-ET was higher than that of s-ET (the survival rates of transferred embryos, 18.8% vs. 10.0%, and the efficiency of embryonic utility, 18.8% vs. 3.7%). Sixtynine percent (20/29) of vitrified-warmed embryos with normal morphology were transferred to 8 ewes by s-ET resulting in a lambing rate of 62.5%. The present results indicate that direct transfer of frozen-thawed ovine embryos is practical, and vitrification is a useful cryopreservation method with less influence on embryonic developmental ability.

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Section: Donor Ewesmentioning

confidence: 99%

Viability of Cryopreserved and Vitrified Embryos and Fertility after Direct Transfer in Ewes.

Okada

Yoshii

Mizuochi

et al. 2002

Journal of Reproduction and Development

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“…From May to June in 2001, 17 donor ewes were treated for estrous synchronization, superovulation and artificial insemination (AI), and embryos were recovered surgically by uterine flushing 5 days after AI [10]. The embryos (morula to blastocyst) were washed in D-PBS containing 0.3% BSA, and were classified for stages and ranks as described in a previous study [1].…”

Section: Embryos Recoverymentioning

confidence: 99%

Birth of Lambs after Direct Transfer of Vitrified Ovine Embryos.

Okada

Wachi

Iida

et al. 2002

Journal of Reproduction and Development

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Abstract. The aim of this study was to investigate in vivo viability of vitrified ovine embryos after direct transfer using a laparoscope. In vivo-derived ovine morulae and blastocysts were equilibrated in 25% glycerol and 25% ethylene glycol solution, loaded into a heat-pulled straw and vitrified. Thirty-eight embryos were transferred into 19 recipients (2 embryos per recipient) and pregnancy was examined at 54-59 days after transfer. The pregnancy, implantation, lambing, and survival rates were 15.8% (3/19), 10.5% (4/38), 15.8% (3/19) and 7.9% (3/38), respectively. The result indicates that viable lambs can be produced by direct transfer of vitrified ovine embryos.

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Results of embryo transfer using laparoscope in sheep

et al. 2009

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Effects of Dosage and Treatment Phase of Two GnRH Analogues at the Estrous Stage on Superovulation in Ewes.

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Cited by 3 publications

References 20 publications

Viability of Cryopreserved and Vitrified Embryos and Fertility after Direct Transfer in Ewes.

Viability of Cryopreserved and Vitrified Embryos and Fertility after Direct Transfer in Ewes.

Birth of Lambs after Direct Transfer of Vitrified Ovine Embryos.

Results of embryo transfer using laparoscope in sheep

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