Effects of Dynorphin A (1-13) on Lipid Peroxidation and the Activity of Antioxidant Enzymes in Immobilization Stress of Different Durations

Abstract

Experiments on rats showed that the selective opioid κ receptor agonist dynorphin A (1-13) had marked antioxidant actions in immobilization stress of different durations, apparent as reductions in lipid peroxidation products in liver tissues. The effects of this peptide on the activities of the antioxidant defense enzymes superoxide dismutase and catalase depended on the duration of stress. While the peptide had a notable stimulatory effect on catalase activity in animals subjected to immobilization for 3 h, this effect was signifi cantly smaller in rats exposed to stress for 6 or 12 h. These studies demonstrated that dynorphin A (1-13) has a stimulatory action on superoxide dismutase activity in animals exposed to immobilization for 6 or 12 h, this being seen at all study time points. Peptide had no effect on the activity of this enzyme in rats exposed to immobilization stress for 3 h.

Impact of Immobilization Stress on Erythropoiesis, Alterations in the Antioxidant System, and Potential Correction with Stevia rebaudiana Bertoni in Rabbit

“…An increase in catalase activity was observed in the liver and kidneys: on the 20th day of stress exposure, it was 16% and 13%, respectively. An increase in catalase activity in the early stages of exposure to various types of stress was also noted by other authors [5][6][7].…”

“…1, B) increases by 21% as compared to the norm, and after 5 days -by 14%. Catalase activity increasing at the early stages of different stress factor effects was mentioned by other authors as well [5,7]. With stress effect in uence enhancement in the chronic stage, the catalase activity gradually decreases.…”

“…As a stress factor, it causes tension in regulatory mechanisms, disruption of vegetative functions reduces the reserve capacity of the body, and inhibits energy processes [3,4]. Short-term exposure to immobilization stress causes erythropenia, a decrease in hemoglobin content and osmotic stability of erythrocytes, and an increase in the number of destroyed erythrocytes in the spleen [5][6][7] against the background of changes in various organs and systems of the body. When exposed to stress factors, the body quickly mobilizes its adaptive, compensatory, and protective mechanisms to counteract the violation of physiological balance, however, prolonged exposure causes anatomical and functional disorders of organ systems, and causes the development of pathological processes [8].…”

Сравнительный Анализ Стимулирующего Действия Селективных Агонистов Опиоидных Рецепторов На Массу Печени Крыс После Обширной Резекции

“…Ðàíåå íàìè ïîêàçàíî, ÷òî îïèîèîäíûå ïåïòèäû àêòèâèðóþò ïðîöåññû ðåãåíåðàöèè â ïå÷åíè ïîñëå ñòðåññ-èíäóöèðîâàííîãî ïîâðåaeäåíèÿ â ýêñïåðèìåíòå. Êàê óñòàíîâëåíî, íàèáîëåå âûðàaeåííûé ñòèìóëèðóþùèé ýôôåêò íàáëþäàëñÿ ïðè âíóòðèáðþøèííîì ïðèìåíåíèè ñåëåêòèâíîãî àãîíèñòà îïèîèäíûõ êàïïà-ðåöåïòîðîâ äèíîðôèíà À (1 -13) â äîçå 20,1 ìêã/êã â òå÷åíèå 5 äíåé ïîñëå ñòðåññîðíîãî âîçäåéñòâèÿ [11,12].…”

“…Ëîaeíîîïåðèðîâàííûì êðûñàì âûïîëíÿëè ñðåäèííóþ  ðàáîòå èñïîëüçîâàíû ñåëåêòèâíûå àãîíèñòû îïèîèäíûõ ðåöåïòîðîâ â ýêâèìîëÿðíûõ äîçàõ: DAGO (àãîíèñò ìþ-ðåöåïòîðîâ, ôèðìà "Sigma-Aldrich", ÑØÀ) â äîçå 6,3 ìêã/êã, DSLET (àãîíèñò äåëüòà-ðåöåïòîðîâ, ëàáîðàòîðèÿ ñèíòåçà ïåïòèäîâ ÍÌÈÖ êàðäèîëîãèè, Ìîñêâà) â äîçå 10 ìêã/êã, äèíîðôèí À (1 -13) (àãîíèñò êàïïà-ðåöåïòîðîâ, ëàáîðàòîðèÿ ñèíòåçà ïåïòèäîâ ÍÌÈÖ êàðäèîëîãèè, Ìîñêâà) â äîçå 20,1 ìêã/êã.  íàøèõ ïðåäøåñòâóþùèõ ýêñïåðèìåíòàõ è ëèòåðàòóðíûõ äàííûõ ïîêàçàíà âûñîêàÿ ýôôåêòèâíîñòü ïðèìåíåíèÿ îïèîèäîâ äëÿ êîððåêöèè ñòðåññ-èíäóöèðîâàííûõ ïîðàaeåíèé ïðè èõ èñïîëüçîâàíèè â óêàçàííûõ äîçàõ [4,11,12]. Ïåïòèäû ââîäèëè âíóòðèáðþøèííî åaeåäíåâíî 1 ðàç â ñóòêè, íà÷èíàÿ ñ ïåðâîãî äíÿ ýêñïåðèìåíòà, â òå÷åíèå 5 äíåé â âèäå âîäíîãî ðàñòâîðà â îáúåìå 0,2 ìë.…”