Effects of ectomycorrhizal fungi on growth and development of poplar plants derived from tissue culture

Effect of ectomycorrhizal fungi on survival and growth of micropropagated plants and seedlings of Castanea sativa mill.

“…Oak and pine seedlings mycorrhizal with Pisolithus tinctorius showed differences in survival related to the extent of mycorrhization of their roots, and seedlings with abundant Pisolithus tinctorius ectomycorrhizas tolerated environmental stresses, such as soil water deficit and high temperatures, better than seedlings without Pisolithus tinctorius ectomycorrhizas (Marx and Cordell 1989). Heslin and Douglas (1986) did not find a significant improvement in the survival of micropropagated poplar plants mycorrhizal with different fungi at the moment of ex vitro transfer, and other reports with micropropagated plants do not refer to survival (Rancillac 1983;Grellier et al 1984;Poissonier 1986;Strullu et al1986). However, other reports on seedling survival and growth after mycorrhization with different fungal species support our conclusions (Boyd et al 1986;Meyer 1987;Feil et al 1988;Le Tacon et al 1992).…”

Thelephora

“…Holden et al (1983), however, compared two Tt isolates from a nursery and a forest. A Tt isolate from Picea has, however, been used to adapt Populus plants from in vitro tissue culture conditions to the glasshouse (Heslin and Douglas 1986). In contrast, inoculation in containers and field testing of Picea sitchensis and Pseudotsuga menziesii with five isolates of Tt indicated no intraspecific differences in growth responses (Jackson et al 1995).…”

In vitro Mycorrhization of Micropropagated Plants: Studies on Castanea sativa Mill

“…Mycorrhization of micropropagated plants before acclimation increases survival, enhancing the functionality of the root system and the mineral plant nutrition (Rancillac, 1982;Grellier et al, 1984;Heslin and Douglas, 1986;Poissonier, 1986;Tonkin et al, 1989;Martins, 1992;2004;Martins et al, 1996;Herrmann et al, 1998;Díez et al, 2000). Similarly, in vitro mycorrhization of micropropagated plants can be used to increase survival and growth during ex vitro weaning (Nowak, 1998).…”

“…Mycorrhization trials have been made with different micropropagated plant species: pine (Rancillac, 1982;Normand et al, 1996), birch (Grellier et al, 1984), poplar (Heslin and Douglas, 1986), eucalyptus (Poissonier, 1986;Tonkin et al, 1989), oak (Herrmann et al, 1998), chestnut (Strullu et al, 1986;Martins, 1992;2004;Martins et al, 1996;Martins and Pais, 2005), cork oak (Díez et al, 2000). These trials were performed as an effort to make micropropagation a sustainable propagation method for plant species recalcitrant to conventional propagation, increasing in vitro plant performances.…”