Effects of EGF receptor ligands on fetal ovine myoblasts

Harper, J.M.M.; Buttery, P. J.

doi:10.1016/s0739-7240(00)00086-2

Cited by 8 publications

(4 citation statements)

References 49 publications

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“…This suggests that EGF plays a stimulating role in murine satellite cell proliferation. Our results are in agreement with previous studies reporting increased proliferation by EGF in the muscle cell lines BC 3 H-1 (Wang and Rubenstein 1988;Kelvin et al 1989), MM14 (Linkhart et al 1982) and L6 and in fetal bovine (Blachowski et al 1993) and ovine (Harper and Buttery 2001) myoblasts. Selection for growth + fitness or purely for growth nearly doubles the mitogenic response to EGF, whereas it is only minimally increased in response to selection for protein amount.…”

Section: Discussionsupporting

confidence: 94%

Intrinsic properties of muscle satellite cells are changed in response to long-term selection of mice for different growth traits

Rehfeldt

Walther²,

Albrecht

et al. 2002

Cell and Tissue Research

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Satellite cell cultures were derived from mice selected long-term over 70 generations for body weight (DU-6, growth), carcass protein amount (DU-6P, protein) and an index combining body weight and endurance treadmill performance (DU-6+LB, growth + fitness) at 42 days of age and from an unselected control line (DU-Ks). They were grown under identical environmental conditions to examine intrinsic cellular differences in proliferation, protein metabolism and responsiveness to growth factors. Growth kinetics (DNA and protein amounts) were determined over a 12-day period. During exponential growth, all growth-selected cultures grew faster than the control culture: (DU-6+LB=DU-6P)>DU-6>DU-Ks. The differences in DNA and protein levels were maintained until day 8. DU-Ks cultures reached similar levels as the growth (DU-6) and protein (DU-6P) cultures in terms of DNA at day 12 of cultivation. Thus, the cultures from the growth and protein lines, but not from the growth + fitness line, exhibited larger protein:DNA ratios (cell size) than the control cultures. Cell cultures from the selected lines were more responsive to serum and epidermal growth factor in terms of [(3)H] thymidine incorporation into DNA, whereas no stimulation by insulin or insulin-like growth factor-I was detectable in cultures from selected lines or controls. During differentiation, protein metabolism in cultures from selected lines was characterised by higher rates of protein synthesis (PS) and degradation (PD), as measured by [(3)H] phenylalanine incorporation or release, respectively, than in control cells. The ratios of the relative differences from the control in PS and PD were only >1.0 in the growth and protein lines. In conclusion, long-term selection for growth therefore modifies the intrinsic capability of satellite cells for proliferation and protein metabolism, with changes being dependent on the selection trait.

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Section: Discussionsupporting

confidence: 94%

Intrinsic properties of muscle satellite cells are changed in response to long-term selection of mice for different growth traits

Rehfeldt

Walther²,

Albrecht

et al. 2002

Cell and Tissue Research

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“…Likewise, activation of Wnt signaling enhances the proliferation of skeletal muscle precursor cells in the chick hindlimb muscles ( Takata et al, 2007 ). Furthermore, EGF signaling positively regulates proliferation in ovine fetal myoblasts ( Harper and Buttery, 2001 ). How these different pathways might converge to achieve the same end goal is not fully understood, however our studies for the first time demonstrate crosstalk between FGF and Wingless signaling in myoblast proliferation, suggesting that multiple signals might converge on the same regulatory module.…”

Section: Discussionmentioning

confidence: 99%

FGF signaling promotes myoblast proliferation through activation of wingless signaling

Vishal

Lovato

Bragg

et al. 2020

Developmental Biology

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Indirect flight muscles (IFMs) are the largest muscles in Drosophila and are made up of hundreds of myonuclei. The generation of these giant muscles requires a large pool of wing disc associated adult muscle precursors (AMPs), however the factors that control proliferation to form this myoblast pool are incompletely known. Here, we examine the role of fibroblast growth factor (FGF) signaling in the proliferation of wing disc associated myoblasts. We find that the components of FGF signaling are expressed in myoblasts and surrounding epithelial cells of the wing disc. Next, we show that attenuation of FGF signaling results in a diminished myoblast pool. This reduction in the pool size is due to decreased myoblast proliferation. By contrast, activating the FGF signaling pathway increases the myoblast pool size and restores the proliferative capacity of FGF knockdown flies. Finally, our results demonstrate that the FGF receptor Heartless acts through up-regulating β-catenin/Armadillo signaling to promote myoblast proliferation. Our studies identify a novel role for FGF signaling during IFM formation and uncover the mechanism through which FGF coordinates with Wingless signaling to promote myoblast proliferation.

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“…As such, HB‐EGF and EGF compete for EGF receptor (EGFR) binding. The EGFR is not expressed in adult differentiated skeletal muscle (Olwin & Haushka, 1988; Harper & Buttery, 2001); however, HB‐EGF may be required for skeletal muscle growth through its interactions with residential non‐muscle cells. For example, a number of physiological roles have been attributed to HB‐EGF in development (Iwamoto et al 2003), angiogenesis (Chalothorn et al 2005), wound healing (Fitsialos et al 2007) and as a chemoattractant (Higashiyama et al 1993).…”

mentioning

confidence: 99%

“…Similar to VEGF, HB‐EGF has also been implicated as a survival factor for differentiating myoblasts (Horikawa et al 1999; Germani et al 2003). Elevated HB‐EGF transcription has been shown in postnatal mouse, rat and human skeletal muscle compared with other tissue types, suggesting an important role during muscle development (Harper & Buttery, 2001). Finally, overexpression of HB‐EGF has been implicated in left ventricular hypertrophy following myocardial infarction (Ushikoshi et al 2005).…”

mentioning

confidence: 99%

Acute vascular endothelial growth factor expression during hypertrophy is muscle phenotype specific and localizes as a striated pattern within fibres

Parvaresh¹,

Huber²,

Brochin³

et al. 2010

Experimental Physiology

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Skeletal muscle hypertrophy requires the co-ordinated expression of locally acting growth factors that promote myofibre growth and concurrent adaptive changes in the microvasculature. These studies tested the hypothesis that vascular endothelial growth factor (VEGF) and heparinbinding epidermal growth factor (HB-EGF) expression are upregulated during the early stages of compensatory muscle growth induced by chronic functional overload (FO). Bilateral FO of the plantaris and soleus muscles was induced for 3 or 7 days in the hindlimbs of adult female Sprague-Dawley rats (n = 5 per group) and compared with control (non-FO) rats. Relative muscle mass (in mg (kg body weight) −1 ) increased by 18 and 24% after 3 days and by 20 and 33% after 7 days in the plantaris and soleus muscles, respectively. No differences in HB-EGF mRNA or protein were observed in either muscle of FO rats relative to control muscles. The VEGF mRNA was similar in the soleus muscles of FO and control rats, whereas a significant elevation occurred at 3 and 7 days of FO in the plantaris muscle. However, VEGF protein expression after 3 days of FO exhibited a differential response; expression in the soleus muscle decreased 1.6-fold, whereas that in the plantaris muscle increased 1.8-fold compared with the control muscle. After 7 days of FO, VEGF protein remained elevated within the plantaris muscle, but returned to basal levels in the soleus. Robust basal HB-EGF and VEGF protein expression was consistently seen in control muscles. In all groups, immunohistochemistry for VEGF protein displayed a distinct striated expression pattern within myofibres, with considerably less labelling in extracellular spaces. Constitutive expression of HB-EGF and VEGF in control myofibres is consistent with housekeeping roles for these growth factors in skeletal muscle tissue. However, the specific patterns of VEGF expression in these muscles during FO may reflect the chronic changes in neural recruitment between muscles and the co-ordination of angiogenic and/or other hypertrophic responses.

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Effects of EGF receptor ligands on fetal ovine myoblasts

Cited by 8 publications

References 49 publications

Intrinsic properties of muscle satellite cells are changed in response to long-term selection of mice for different growth traits

Intrinsic properties of muscle satellite cells are changed in response to long-term selection of mice for different growth traits

FGF signaling promotes myoblast proliferation through activation of wingless signaling

Acute vascular endothelial growth factor expression during hypertrophy is muscle phenotype specific and localizes as a striated pattern within fibres

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