Effects of egg shell quality and washing on Salmonella Infantis penetration

Samiullah, -; Chousalkar, Kapil K.; Roberts, Juliet R.; Sexton, Margaret; May, Damian; Kiermeier, Andreas

doi:10.1016/j.ijfoodmicro.2013.05.002

Cited by 45 publications

(18 citation statements)

References 30 publications

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“…Statistical analysis also showed that, in most cases, shell thickness was not related to the eggshell penetration. Similarly, a number of studies have observed that the shell thickness did not affect whole egg and agar egg penetration [16], [18], [33].…”

Section: Discussionmentioning

confidence: 87%

“…Shell pieces of approximately 1 cm 2 were cut out from around the equator of the eggshell using a Dremel tool, mounted on a 9 mm diameter aluminium stub, sputter coated and viewed under the SEM, as described above. Scoring of the cuticle was done according to Samiullah et al [16] with following criteria: Cuticle score 1 = 90 to 100% cuticle cover, score 2 = 60 to 90% cuticle cover, score 3 = 20 to 60% cuticle cover, score 4 = 0 to 10% cuticle cover.…”

Section: Methodsmentioning

confidence: 99%

“…However, there is a lack of information available regarding how well these S. Typhimurium strains survive on the eggshell surface and penetrate the shell to contaminate the internal contents of the egg. A preliminary study suggested that eggshell ultrastructure may have an impact on eggshell penetration by Salmonella [16], but there is a lack of direct evidence.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Egg Washing and Correlation between Eggshell Characteristics and Egg Penetration by Various Salmonella Typhimurium Strains

et al. 2014

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Salmonella is an important foodborne pathogen, causing an estimated 11,992 cases of infection in Australia per year. Egg or egg product related salmonellosis is a major concern for the egg industry. Worldwide, S. Typhimurium is one of the most common serovars identified in Salmonella food poisoning cases. The current study investigated the ability of five S. Typhimurium strains to penetrate washed and unwashed eggs using whole egg and agar egg penetration methods. All S. Typhimurium strains were able to penetrate eggshells and survive in egg albumen (at 20°C) according to whole egg penetration results. Polymerase Chain Reaction results demonstrated that S. Typhimurium strain 2 (103 and 105 CFU/mL), and strain 5 (103 and 105 CFU/mL) egg penetration was significantly higher (p<0.05) in washed eggs when compared to unwashed eggs. Statistical analysis of the agar penetration experiment indicated that S. Typhimurium was able to penetrate washed eggs at a significantly higher rate when compared to unwashed eggs (p<0.05). When compared to unwashed eggs, washed eggs also had significantly damaged cuticles. Statistical analysis also indicated that eggshell penetration by S. Typhimurium was related to various eggshell ultrastructural features such as cap quality, alignment, erosion, confluence, Type B bodies and cuticle cover.

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Section: Discussionmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Egg Washing and Correlation between Eggshell Characteristics and Egg Penetration by Various Salmonella Typhimurium Strains

et al. 2014

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“…Salmonella organisms could penetrate the intestines mucosal epithelium to outcompete the resident microbial flora and cause the pathological reaction [7]. Egg-related salmonellosis is costing $44 million per year in Australia [8]. …”

Section: Introductionmentioning

confidence: 99%

Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer

Liu

et al. 2017

BMC Genomics

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Background Salmonella enterica serovar Enteritidis (SE) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. MicroRNAs (miRNAs) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. The objective of this study is to identify miRNAs associated with SE infection in laying chicken cecum.ResultsAverage number of reads of three libraries constructed from infected and non-infected chickens was 12,476,156 and 10,866,976, respectively. There were 598 miRNAs including 194 potential novel miRNAs identified in which 37 miRNAs were significantly differentially expressed between infected and non-infected chickens. In total, 2897 unique target genes regulated by differentially expressed miRNAs were predicted, in which, 841 genes were uniquely regulated by up-regulated miRNAs (G1), 636 genes were uniquely regulated by down-regulated miRNAs (G2), and 1420 were co-regulated by both up and down- regulated miRNAs (G3). There were 118, 73 and 178 GO (Gene ontology) BP (Biological process) terms significantly enriched in G1, G2 and G3 groups, respectively. More immune-related GO BP terms than metabolism-related terms were found in G1. Expression of 12 immune-related genes of four differentially expressed miRNAs was detected through qRT-PCR. The regulatory direction of gga-miR-1416-5p, gga-miR-1662, and gga-miR-34a-5p were opposite with the target genes of TLR21, BCL10, TLR1LA, NOTCH2 and THBS1, respectively.ConclusionThe miRNAs contribute to the response to SE infection at the onset of egg laying through regulating the homeostasis between metabolism and immunity. The gga-miR-125b-5p, gga-miR-34a-5p, gga-miR-1416-5p and gga-miR-1662 could play an important role in SE infection through regulating their target genes. The finding herein will pave the foundation for the studies of microRNA regulation in SE infection in laying hens.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3413-8) contains supplementary material, which is available to authorized users.

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“…Such occurrence poses risks to the consuming public (Murchie et al, 2008), especially with the increased desire of the consumers for raw and unprocessed eggs, potential risk of salmonellosis Quilloy et al 919 (Whiley and Ross, 2015). In Australia, the impact of eggrelated salmonellosis was estimated to be 44 million dollars (Samiullah et al, 2013). Automation of collecting processes and mechanization of sorting in high-end egg farms are able to minimize contact with human hands.…”

Section: Introductionmentioning

confidence: 99%

Single-line automated sorter using mechatronics and machine vision system for Philippine table eggs

Quilloy¹,

Suministrado²,

Bato³

2018

Afr. J. Agric. Res.

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An automated single-line table egg sorting machine that integrates machine vision and mechatronics principles was developed in this study. The machine was fabricated using low cost and locally available materials. The developed machine was composed of the feeding unit, computing unit and the sorting unit. The conveyor was powered by a 12V DC geared motor, and the sorting arm was actuated by a DC servo motor which positions the arm. A machine vision software, EGGSoTiC, was used in the sorting of the table eggs -moving through a conveyor at 13 cm-s -1. Test for similarity of readings revealed that the developed machine is capable of yielding consistent results with low values of coefficient of variation ranging from 0.38 to 0.85 mm 2 and 0.42 to 0.94 g for the projected area and estimated weight, respectively. Results of dynamic test for 100 table egg samples indicated that the machine could sort table eggs with an accuracy of 91% at 2.52 seconds per sample, yielding a projected capacity of 1,426 eggs per hour. Results also indicated that there were no large errors in the estimation of weights yielding a root mean square error of 1.90 g which is not significantly higher than the bias of 0.93 g.

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Effects of egg shell quality and washing on Salmonella Infantis penetration

Cited by 45 publications

References 30 publications

Effect of Egg Washing and Correlation between Eggshell Characteristics and Egg Penetration by Various Salmonella Typhimurium Strains

Effect of Egg Washing and Correlation between Eggshell Characteristics and Egg Penetration by Various Salmonella Typhimurium Strains

Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer

Single-line automated sorter using mechatronics and machine vision system for Philippine table eggs

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