Effects of egg yolk antibody against Porphyromonas gingivalis gingipains in periodontitis patients

Abstract

Porphyromonas gingivalis gingipains issuspected to be one of the most important causative agents of periodontitis. We postulated that the inhibition of gingipains may reduce the pathogenic nature of P. gingivalis. Anti-P. gingivalis egg yolk antibody (IgY-GP) was isolated from the yolks of hens immunized with purified gingipains. We applied IgY-GP gel subgingivally in periodontitis patients who harbored P. gingivalis in their subgingival flora. Five pairs of contralateral anterior single-rooted teeth were selected. One tooth in each contralateral pair was randomly treated with IgY-GP and subgingival scaling and root planing, whereas the other tooth was treated with SRP alone. The number of P. gingivalis bacteria was assessed by real-time PCR. Bacterial levels were expressed as the percentage of total bacteria. The IgY-GP group had a significant reduction in probing depth. BOP significantly decreased in the IgY-GP group compared to the control group at week 4. The levels of P. gingivalis significantly increased in the control group at week 4, whereas the reduction in the levels of P. gingivalis was sustained in the IgY-GP group. Within the limitations of the present study, IgY-GP was shown to be an effective immunotherapeutic agent in the treatment of periodontitis. (J. Oral Sci. 49,[201][202][203][204][205][206] 2007)

Anti-Pseudomonas aeruginosaIgY antibodies promote bacterial opsonization and augment the phagocytic activity of polymorphonuclear neutrophils

“…27 Human clinical studies on the efficacy of prophylactic IgY therapy have primarily targeted gastrointestinal infections. Oral pathogens causing dental caries, 28 periodontitis 29 and oral candidiasis 30 have been successfully reduced with pathogen-specific IgY. Infectious diseases caused by important gastrointestinal pathogens like Helicobacter pylori 31 and rotavirus 32 are diminished clinically with oral passive IgY immunotherapy.…”

Antibodies against Venom of the Snake Deinagkistrodon acutus

“…In addition to these advantages, a chicken can provide more than 40 g IgY per year (15). Thus far, numerous studies on IgY used for immunotherapy in clinical and experimental treatments have been reported (16)(17)(18)(19)(20). However, poly-clonal antibodies, including IgY, are prone to cross-reactions and are of inconsistent quality.…”

Periodontal Vaccine—A Boon In Periodontics

“…[13]- [16] HRgpA (90kDa) and RgpB(50kDa) products of 2 distinct but related genes rgpA and rgpB respectively are specific for Arg-Xaa peptide bonds. Kgp,a product kgp genes is specific for Lys-Xaa peptide bonds.…”