Effects of Eggshell Membrane on Keratinocyte Differentiation and Skin Aging In Vitro and In Vivo

Furukawa, Kyohei; Kono, Michiko; Kataoka, Tateshi; Hasebe, Yukio; Jia, Huijuan; Kato, Harubumi

doi:10.3390/nu13072144

Cited by 8 publications

(7 citation statements)

References 31 publications

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“…Yoo et al reported the anti-wrinkle, anti-inflammatory, and antimicrobial activities of ESM hydrolysates (Yoo et al 2014). Furthermore, the effects of oral ingestion of ESM on skin aging and differentiation have been studied (Furukawa et al 2021). However, the mechanism underlying the antiaging effect of ESM on the skin was not clearly identified in these studies.…”

Section: Discussionmentioning

confidence: 99%

Anti-skin aging activity of eggshell membrane administration and its underlying mechanism

Sim

Ahn

Lim

et al. 2022

Mol. Cell. Toxicol.

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Background There is active research on developing materials for improving skin function. Eggshell membrane (ESM) is one such raw material that is consumed as a functional food to support skin health. However, studies on the mechanism of improvement of skin function on ingestion of ESM are still lacking. Objectives To explore this mechanism of action, we conducted an ultraviolet (UV) irradiation study on a SKH-1 hairless mouse model. Feeding ESM was found to improve skin moisture and reduce wrinkles during 12 weeks of UVB irradiation. Results Oral administration of ESM restored moisture in the dorsal skin tissue of mice. In addition, oral ingestion of ESM also reversed the increased transepidermal water loss and reduction of mRNA expression of hyaluronic synthases induced by UVB irradiation. Furthermore, UVB irradiation-induced collagen degradation was inhibited, and the expression of the collagenase MMP was reduced in the ESM intake group compared to the control. These results confirmed that oral ingestion of the ESM has an anti-wrinkle effect. In addition, the mRNA expression of the antioxidant enzyme SOD1, which was reduced on UVB irradiation, was restored on ingestion of the ESM. Restoring the expression of antioxidant enzymes is a key strategy for improving skin function of the ESM. Conclusion Taken together, the findings from our study reveal the potential of ESM as a nutricosmetic material with anti-wrinkle and skin moisturizing properties.

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Section: Discussionmentioning

confidence: 99%

Anti-skin aging activity of eggshell membrane administration and its underlying mechanism

Sim

Ahn

Lim

et al. 2022

Mol. Cell. Toxicol.

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show abstract

“…Total RNA from the tibialis anterior muscle of three mice, which had a body and muscle weight close to the average value, was used for DNA microarray analysis according to previous studies [ 17 , 18 ]. RNA samples (250 ng) were reverse transcribed into single-stranded cDNA using GeneChip ® 3′IVT Express Kit (Thermo Fisher Scientific).…”

Section: Methodsmentioning

confidence: 99%

Suppressive Effects of Turmeric Extract on Muscle Atrophy in Dexamethasone-Treated Mice and Myotubes

Furukawa¹,

Kousaka²,

Jia³

et al. 2022

Nutrients

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Sarcopenia is the decline in skeletal muscle mass, strength, and functions, which decreases the quality of life in elderly people. This study investigated the suppressive effect of turmeric (Curcuma longa) extract (TE) on muscle atrophy in dexamethasone (DEX)-treated mice and C2C12 myotubes. DEX treatment significantly decreased the muscle weight and significantly increased Fbxo32 and Murf1 expression in mice, and these changes were suppressed by the supplementation of an AIN-93 based diet with 2% TE. A similar pattern was observed in FBXO32 and MuRF1 protein expression. In C2C12 myotubes, DEX treatment significantly increased FBXO32 and MuRF1 gene and protein expression, and these increases were significantly suppressed by TE supplementation at a concentration of 200 µg/mL. Furthermore, one of the five TE fractions, which were separated by high-performance liquid chromatography had a similar effect with TE supplementation. The present study proposes the suppressive effect of turmeric on sarcopenia.

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“…Furthermore, telomerase transcriptase (Tert) expression shows that ESM can improve telomere shortening, which is a sign of aging. The expression of growth factors indicates the possibility of ESM influencing genes associated with inflammation (34).…”

Section: Benefits Of Eggshell On Skin Tissuementioning

confidence: 99%

Review of the Benefits of Eggshell Content in Body Tissue Structure Repair

Herman¹,

Minhajat²,

Muis³

et al. 2023

MJMHS

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Eggshell (ES) is a waste material that cannot be consumed with low economic value. ES mainly contains calcium carbonate (CaCO3) and an organic matrix in the form of proteins, glycoproteins, and proteoglycans. Meanwhile, the eggshell membrane (ESM) contains osteopontin collagen, fibronectin, keratin, histones, avian beta defensins, ovocalyxin-36, apolipoproteins, protocadherin, chondroitin sulfate, ovotransferrin, hyaluronic acid, and sialic acid as well as various amino acids. Recently, ES has been widely used in industry, agriculture, food, and medical fields. The potential of ES in the medical field is interesting to discuss, especially in relation to tissue repair. Efforts to reduce the prevalence of wounds that generally originate from acute wounds but become chronic due to various factors that are neglected in their management. In particular, this review will describe the benefits of ES content in repairing body tissues. ES-derived active ingredients such as CaCO3, brushite, and hydroxyapatite exhibit osteoconductive properties that promote bone regeneration. Calcium ions can increase insulin and leptin sensitivity in the liver and can induce repair of acute kidney injury. Meanwhile, ESM contributes positively to neural tissue repair and plays an important role in wound healing, response to external stimuli, defense response, inflammatory response, cell-substrate adhesion, promoting cell growth, migration, differentiation, and tissue remodeling.

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Effects of Eggshell Membrane on Keratinocyte Differentiation and Skin Aging In Vitro and In Vivo

Cited by 8 publications

References 31 publications

Anti-skin aging activity of eggshell membrane administration and its underlying mechanism

Anti-skin aging activity of eggshell membrane administration and its underlying mechanism

Suppressive Effects of Turmeric Extract on Muscle Atrophy in Dexamethasone-Treated Mice and Myotubes

Review of the Benefits of Eggshell Content in Body Tissue Structure Repair

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