In the present study, detailed comparative experiments on a heavy-duty diesel engine used in the world harmonized transient cycle (WHTC) and road spectrum reversely deduced cycle (RSRDC, which was derived from a road test) were carried out. Fuel consumption and gaseous and particulate pollutants, along with some engine operation parameters, were measured transiently; thus, specific emissions can be calculated. Results showed that the BSFC of WHTC and RSRDC was 201.8 and 210 g/kW·h, respectively, because the real road driving cycle (RSRDC) had wider operating point distributions and more points located in the low-efficiency zone relative to WHTC. Thus, WHTC operations exhibited higher raw CO (abundant CO formation needed a specific temperature threshold) and NOx but lower HC. Furthermore, with aftertreatment, all pollutants met the newest China regulation limit. Finally, transient emissions were analyzed in detail. Although the specific emissions of some pollutants were similar in value for both cycles, transient processes may largely be different. Therefore, the current study is meaningful, and we not only provide broad and detailed information but also directly compare two types of operations (one is a real road driving cycle) in the laboratory: this is rarely discussed in the literature.