Effects of electrospun scaffolds of di-O-butyrylchitin and poly-(ε-caprolactone) on wound healing

Abstract

Background:We sought to determine the usefulness of electrospun dibutyrylchitin (DBC) or poly-(ε-caprolactone [PCL]), in wound treatment. We investigated the mechanisms of action of these polymers on wound healing. Methods:We synthesized DBC, a newly identified ester derivative of chitin, using a patented method comprising the substitution of butyryl groups at positions C-3 and C-6 in chitin molecules. We confirmed the double substitution by the butyric groups using infrared spectrometry. The fibrous scaffolds were obtained using the electrospinning method. A polypropylene net was implanted subcutaneously in the rat and served as a wound model. Results:Both DBC and PCL increased granulation tissue weight in the wound. In contrast to PCL, DBC did not abolish glycosaminoglycan changes in wounds. The tested samples did not impair total collagen synthesis or induce excessive fibrosis. In both PCL-and DBC-treated wounds, we observed a lower level of soluble collagen (compared with controls). The results show better hydration of the wounds in both the DBC and PCL groups. No induction of large edema formation by the tested materials was observed. These polymers induced almost identical macrophagemediated reactions to foreign-body implantation. The implants increased the blood vessel number in a wound. Conclusion:Both PCL and DBC could be used as scaffolds or dressings for wound treatment. The materials were safe and well tolerated by animals. As DBC did not disturb glycosaminoglycan accumulation in wounds and absorbed twice as much liquid as PCL, it can be considered superior.Contexte : Nous avons cherché à déterminer l'utilité du dibutyryl-chitine (DBC) ou du poly-(ε-caprolactone [PCL]) électrofilés dans le traitement des plaies. Nous avons étudié les mécanismes d'action de ces polymères sur la cicatrisation des plaies.Méthodes : Nous avons synthétisé le DBC, un dérivé ester récemment identifié de la chitine, à l'aide d'une méthode brevetée incluant la substitution des groupes butyryl aux positions C-3 et C-6 des molécules de chitine. Nous avons confirmé la substitution double par les groupes butyriques à l'aide de la spectrométrie infrarouge. Les échafaudages fibreux ont été obtenus grâce à la méthode de filage électrostatique. Un filet en polypropylène a été implanté par voie sous-cutanée dans le rat et a servi de modèle de plaie. Résultats :Le DBC et le PCL ont tous deux augmenté le poids du tissu de granulation dans la plaie. Contrairement au PCL, le DBC n'a pas supprimé les changements des glycosaminoglycanes des plaies. Les échantillons examinés n'ont pas perturbé la synthèse totale de collagène ni entraîné une fibrose excessive. Nous avons observé un niveau inférieur de collagène soluble (par rapport aux témoins) tant dans les plaies traitées par PCL que par DBC. Les résultats montrent une amélioration de l'hydratation des plaies tant pour les groupes DBC que PCL. Les matériaux à l'étude n'induisaient pas d'oedème étendu. Ces polymères ont induit des réactions macrophagiques presque identiques à l'implantation ...

Influence of Porous Dressings Based on Butyric-Acetic Chitin Co-Polymer on Biological Processes In Vitro and In Vivo

“…In the early stage, it accelerated the epithelisation process, it evoked slight inflammatory effect with delicate granulocytes induction (eosins connected to inflammation reaction), reduced the effusion phase and accelerated the creation phase. Similar histological image for the healing process was shown for the tests in which di-O-butyryl chitin (DBC) was used [47], and in which delicate inflammatory effect with macrophages presence, increase of vascularisation and then fibrosis was observed.…”

Optimizing the nanostructure of graphene oxide/silver/arginine for effective wound healing

“…A slight decrease in wound size demonstrated that the toxic effect of Ag nanoparticles dramatically diminishes the healing capability of PCL. In fact, PCL improves wound healing through the induction of macrophage-mediated reactions, which produces growth factors [83].…”

Gene therapy in wound healing using nanotechnology

“…Nanofibers alone were also able to enhance cell attachment and proliferation through ECM scaffold mimicry while the addition of various growth factors promoted re‐epithelialization and angiogenesis 43 . Drobnik et al explored the efficacy of DBC and PCL scaffolds using murine models, of which both materials increased wound granulation tissue, improved hydration, and angiogenesis 78 . Beneficial effects are also seen in diabetic wounds where hybrid nanofibers functionally replace the lost ECM to facilitate fibroblasts and keratinocytes growth and actions 79 .…”

“…43 Drobnik et al explored the efficacy of DBC and PCL scaffolds using murine models, of which both materials increased wound granulation tissue, improved hydration, and angiogenesis. 78 Beneficial effects are also seen in diabetic wounds where hybrid nanofibers functionally replace the lost ECM to facilitate fibroblasts and keratinocytes growth and actions. 79 Other substances such as beta-glucan and PVA-PEG hybrid hydrogels were also feasible combinations that provided significant improvements in animal wound models.…”