Effects of elevated CO2 and nitrogen supply on the growth and photosynthetic physiology of a marine cyanobacterium, Synechococcus sp. PCC7002

Mou, Shanli; Zhang, Yongyu; Li, Gang; Li, Hongmei; Liang, Yantao; Tang, Lili; Tao, Jianchang; Xu, Jianjun; Li, Jia; Zhang, Chuanlun; Jiao, Nianzhi

doi:10.1007/s10811-017-1089-3

Cited by 18 publications

(11 citation statements)

References 53 publications

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“…Interestingly, BPNPs in Synechococcus 7002 were much smaller than those in other reported cyanobacteria. This could probably be explained by Synechococcus 7002 being a rather smaller cyanobacterium with an average cell diameter of only 750 nm [ 25 ]. Particles smaller than 100 nm have been defined as nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro

et al. 2018

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Probiotic-derived polyphosphates have attracted interest as potential therapeutic agents to improve intestinal health. The current study discovered the intracellular accumulation of polyphosphates in a marine cyanobacterium Synechococcus sp. PCC 7002 as nano-sized granules. The maximum accumulation of polyphosphates in Synechococcus sp. PCC 7002 was found at the late logarithmic growth phase when the medium contained 0.74 mM of KH2PO4, 11.76 mM of NaNO3, and 30.42 mM of Na2SO4. Biogenic polyphosphate nanoparticles (BPNPs) were obtained intact from the algae cells by hot water extraction, and were purified to remove the organic impurities by Sephadex G-100 gel filtration. By using 100 kDa ultrafiltration, BPNPs were fractionated into the larger and smaller populations with diameters ranging between 30–70 nm and 10–30 nm, respectively. 4′,6-diamidino-2-phenylindole fluorescence and orthophosphate production revealed that a minor portion of BPNPs (about 14–18%) were degraded during simulated gastrointestinal digestion. In vitro studies using lipopolysaccharide-activated RAW264.7 cells showed that BPNPs inhibited cyclooxygenase-2, inducible nitric oxide (NO) synthase expression, and the production of proinflammatory mediators, including NO, tumor necrosis factor-α, interleukin-6, and interleukin-1β through suppressing the Toll-like receptor 4/NF-κB signaling pathway. Overall, there is promise in the use of the marine cyanobacterium Synechococcus sp. PCC 7002 to produce BPNPs, an anti-inflammatory postbiotic.

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Section: Resultsmentioning

confidence: 99%

Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro

et al. 2018

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“…As a result of ongoing fossil fuel combustion, atmospheric CO 2 partial pressure ( p CO 2 ) is predicted to nearly double by the end of this century (Stocker et al, 2013; Friedlingstein et al, 2014). Elevated p CO 2 may affect phytoplankton primary production (Shi et al, 2017), growth rates (Boatman et al, 2017), cell size (Finkel et al, 2010; Mou et al, 2017) and can lead to enhanced cellular carbon to nutrient ratios (Fu et al, 2007; Van de Waal et al, 2010; Garcia et al, 2011). Increased p CO 2 , together with other greenhouse gasses, has caused an increase in the global mean temperature and led to warming of the upper water layers of lakes and oceans, which may in turn enhance thermal stratification (Stocker et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Cyanophage Propagation in the Freshwater Cyanobacterium Phormidium Is Constrained by Phosphorus Limitation and Enhanced by Elevated pCO2

et al. 2019

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Intensification of human activities has led to changes in the availabilities of CO 2 and nutrients in freshwater ecosystems, which may greatly alter the physiological status of phytoplankton. Viruses require hosts for their reproduction and shifts in phytoplankton host physiology through global environmental change may thus affect viral infections as well. Various studies have investigated the impacts of single environmental factors on phytoplankton virus propagation, yet little is known about the impacts of multiple factors, particularly in freshwater systems. We therefore tested the combined effects of phosphorus limitation and elevated p CO 2 on the propagation of a cyanophage infecting a freshwater cyanobacterium. To this end, we cultured Phormidium in P-limited chemostats under ambient (400 μatm) and elevated (800 μatm) p CO 2 at growth rates of 0.6, 0.3, and 0.05 d -1 . Host C:P ratios generally increased with strengthened P-limitation and with elevated p CO 2 . Upon host steady state conditions, virus growth characteristics were obtained in separate infection assays where hosts were infected by the double-stranded DNA cyanophage PP. Severe P-limitation (host growth 0.05 d -1 ) led to a 85% decrease in cyanophage production rate and a 73% decrease in burst size compared to the 0.6 d -1 grown P-limited cultures. Elevated p CO 2 induced a 96% increase in cyanophage production rate and a 57% increase in burst size, as well as an 85% shorter latent period as compared to ambient p CO 2 at the different host growth rates. In addition, elevated p CO 2 caused a decrease in the plaquing efficiency and an increase in the abortion percentage for the 0.05 d -1 P-limited treatment, while the plaquing efficiency increased for the 0.6 d -1 P-limited cultures. Together, our results demonstrate interactive effects of elevated p CO 2 and P-limitation on cyanophage propagation, and show that viral propagation is generally constrained by P-limitation but enhanced with elevated p CO 2 . Our findings indicate that global change will likely have a severe impact on virus growth characteristics and thereby on the control of cyanobacterial hosts in freshwater ecosystems.

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“…We wondered whether this transfer of nutrients could be affected by potential changes in cyanobacterium biomass caused by a modified atmosphere: differences in culture conditions (including carbon and nitrogen availability) can lead to different biomass compositions in microalgae (see Jiang et al, 2012 ; Juneja et al, 2013 ; Mou et al, 2017 ). As an example, growing A. cylindrica under a low-pressure, high-CO 2 atmosphere (fixed nitrogen was provided in the medium) caused an increase in the carbohydrate contents, and a decrease in the protein contents, of its biomass (K. Lehto, reported as unpublished results in Verseux et al, 2016a ).…”

Section: Discussionmentioning

confidence: 99%

A Low-Pressure, N2/CO2 Atmosphere Is Suitable for Cyanobacterium-Based Life-Support Systems on Mars

et al. 2021

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The leading space agencies aim for crewed missions to Mars in the coming decades. Among the associated challenges is the need to provide astronauts with life-support consumables and, for a Mars exploration program to be sustainable, most of those consumables should be generated on site. Research is being done to achieve this using cyanobacteria: fed from Mars's regolith and atmosphere, they would serve as a basis for biological life-support systems that rely on local materials. Efficiency will largely depend on cyanobacteria's behavior under artificial atmospheres: a compromise is needed between conditions that would be desirable from a purely engineering and logistical standpoint (by being close to conditions found on the Martian surface) and conditions that optimize cyanobacterial productivity. To help identify this compromise, we developed a low-pressure photobioreactor, dubbed Atmos, that can provide tightly regulated atmospheric conditions to nine cultivation chambers. We used it to study the effects of a 96% N2, 4% CO2 gas mixture at a total pressure of 100 hPa on Anabaena sp. PCC 7938. We showed that those atmospheric conditions (referred to as MDA-1) can support the vigorous autotrophic, diazotrophic growth of cyanobacteria. We found that MDA-1 did not prevent Anabaena sp. from using an analog of Martian regolith (MGS-1) as a nutrient source. Finally, we demonstrated that cyanobacterial biomass grown under MDA-1 could be used for feeding secondary consumers (here, the heterotrophic bacterium E. coli W). Taken as a whole, our results suggest that a mixture of gases extracted from the Martian atmosphere, brought to approximately one tenth of Earth's pressure at sea level, would be suitable for photobioreactor modules of cyanobacterium-based life-support systems. This finding could greatly enhance the viability of such systems on Mars.

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Effects of elevated CO2 and nitrogen supply on the growth and photosynthetic physiology of a marine cyanobacterium, Synechococcus sp. PCC7002

Cited by 18 publications

References 53 publications

Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro

Biogenic Polyphosphate Nanoparticles from a Marine Cyanobacterium Synechococcus sp. PCC 7002: Production, Characterization, and Anti-Inflammatory Properties In Vitro

Cyanophage Propagation in the Freshwater Cyanobacterium Phormidium Is Constrained by Phosphorus Limitation and Enhanced by Elevated pCO2

A Low-Pressure, N2/CO2 Atmosphere Is Suitable for Cyanobacterium-Based Life-Support Systems on Mars

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