Effects of elicitors on secondary metabolite (SM) production and antioxidant activity in sweet basil (Ocimum basilicum L.) cell suspension cultures

Abstract

Elicitor treatments play an important role in inducing some protective signal transmitter enzymes in cells and regulating phenylalanine ammonia-lyase (PAL) activity. The aim of this study was to determine the effects of elicitors [silver nitrate (AgNO3), salicylic acid (SA) and yeast extract (YE)], which were added individually or in combination to Ocimum basilicum L. cell suspension cultures, on the production and antioxidant activity of secondary metabolites (SMs). Calluses were obtained from the leaves of O. basilicum kept on Murashige and Skoog (MS) medium containing 0.5 mg/l kinetin (KIN) + 2.5 mg/l naphthalene-acetic acid (NAA) and cell suspension cultures were initiated. Then elicitors were applied to the cell suspension cultures individually or in combination, and cells were harvested at the end of the second, fourth, and eighth days. Compared with the control culture, the maximum rosmarinic and chicoric acid production was obtained at the end of the 4th day from SA (24 µM) + YE (80 mg/l) treatment as 20.19 mg/g DW (118%) and 7.55 mg/g DW (123%), respectively. The maximum biosynthesis of isoquercetin and rutin compared with the control culture was 3.88 mg/g DW [YE (80 mg/l)] with a 1.6-fold increase and 6.35 mg/g DW [YE (80 mg/l) + AgNO3 (6 µM)] with a 1.76-fold increase, respectively. Estragole and linalool's highest values compared with the control culture were 4.50 μg/g DW [AgNO3 (6µM) + SA (24 µM)] and 3.02 μg/g DW [SA (24 µM)], respectively. Results clearly show that the elicitor treatment could enhance the biosynthesis of phenolic compounds and terpenoid content in cell suspension cultures of O. basilicum and may be used for commercial supply in the future for therapeutic applications.

Cydonia oblonga Mill. Pulp Callus Inhibits Oxidative Stress and Inflammation in Injured Cells

“…Plants, including quince, are a valuable source of chemical compounds, called secondary metabolites (SMs), which are frequently used as the primary source in the development of food additives, functional foods, and new drugs thanks to their ability to exert several activities useful for human health, such as antibacterial, antiviral, anti-tumoral, antioxidant, anticonvulsant, analgesic, anti-inflammatory, and antidepressant effects [1,2]. These molecules are produced in many varieties of plants, but their natural production is modest and un-homogeneous due to a variety of factors, such as season, environment, climate, and cultivar, which make their extraction from plant matrices difficult and require the use of solvent, steam, and supercritical fluids [3]. At the same time, for SMs production, it is necessary to resort to chemical synthesis or the use of large quantities of water and fruit material.…”

Callus formation and camphor aggregation in response to sorbitol stimulated osmotic stress in yarrow

“…Classical plant production methods and the extraction of secondary metabolites prove more resource-intensive and time-consuming compared to cell cultures. Hence, tissue culture techniques, particularly callus culture, emerge as popular methods for secondary metabolite production (Açıkgöz et al, 2018a;Açıkgöz et al, 2018b;Açıkgöz et al, 2022;Dağlioğlu et al, 2022;Ebru et al, 2022;Açıkgöz et al, 2023). Callus culture efficiently generates products of specific quality and standards, enhances genetic diversity, introduces new compounds absent in the mother plant, optimizes space utilization, and utilizes minimal resources.…”

Türk Civanperçemi (Achillea gypsicola Hub. Mor.) Hücre Süspansiyon Kültürlerinde Fenilpropanoid Metabolitlerin Işık Aracılığıyla Biosentezi

“…Therefore, there is a need to develop some methods to support the production of high-value bioactive metabolites in terms of quantity and quality. Among these methods, plant cell culture techniques to be used for secondary metabolite production is of great importance (Batı Ay et al, 2022). Further, plant cell cultures reduce the demand for expensive chemicals due to their simple extraction methods.…”