Effects of Elicitors on the Production of Resveratrol and Viniferins in Cell Cultures of <i>Vitis vinifera</i> L. cv Italia

Santamaría, Anna; Mulinacci, Nádia; Valletta, Alessio; Innocenti, Marzia; Pasqua, Gabriella

doi:10.1021/jf201181n

Cited by 73 publications

(52 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MeJA is an important plant regulator involved in fruit ripening, senescence, vegetative growth, cell cycle regulation, and plant defense mechanisms in response to herbivores, pathogen attack, and environmental stresses (Liechti and Farmer 2002;Wasternack and Hause 2002;Castillo and León 2008;Pauwels and Goossens 2011). The observed strong upregulation of VvPAL1 mediated by MeJA, is in line with previous reports showing that this hormone promotes the accumulation of several phenolic compounds including phytoalexins, anthocyanins, resveratrol and viniferins, and also induces sesquiterpene biosynthesis in grape cells (Krisa et al 1999;Righetti et al 2007;D'Onofrio et al 2009;Qu et al 2011;Santamaria et al 2011Santamaria et al , 2012. In addition, MeJA was shown to activate the biosynthesis of terpenoids in the leaves of Vitis cv.…”

Section: Vvgpt1 Is Likely Involved In Starch Accumulation In Heterotrsupporting

confidence: 70%

Identification and functional characterization of grapevine transporters that mediate glucose-6-phosphate uptake into plastids

Noronha

Conde

Delrot

et al. 2015

Planta

View full text Add to dashboard Cite

Two grapevine glucose-6-Pi plastidial transporters differently expressed in plant organs and in response to environmental and hormonal signals are characterized. They are involved in starch accumulation in berries and canes. In grapevine, starch accumulation in the trunk is important for winter storage of carbon and in the flower for reproductive development. Berries also accumulate starch in their plastids, which are also involved in the synthesis of aroma compounds important for fruit quality. The present work characterizes two glucose-phosphate translocators (VvGPT1, VvGPT2) that control the accumulation of starch in grape amyloplasts. Three different splicing variants identified for VvGPT2 (VvGPT2α, VvGPT2β and VvGPT2Ω) were more expressed in the leaves than in other organs. In contrast, VvGPT1 transcripts were more abundant in mature berries, canes and flowers than in the leaves. Expression of 35S-VvGPT1-GFP and 35S-VvGPT2Ω-GFP in tobacco leaf epidermal cells showed that the fusion proteins localized at the plastidial envelope. Complementation of the Arabidopsis pgi1-1 mutant impaired in leaf starch synthesis restored its ability to synthesize starch, demonstrating that VvGPT1 and VvGPT2Ω mediate the transport of glucose-6-Pi across the plastidial envelope. In grape cell suspensions, ABA, light and galactinol, together with sucrose and fructose, significantly increased the transcript abundance of VvGPT1, whereas VvGPT2Ω expression was affected only by sucrose. In addition, elicitation with methyl jasmonate strongly upregulated VvGPT1, VvGPT2Ω and VvPAL1, suggesting a role for GPTs in the production of secondary compounds in grapevine. Moreover, in grapevines cultivated in field conditions, VvGPT1 expression was higher in berries more exposed to the sun and subjected to higher temperatures. Although both VvGPT1 and VvGPT2 mediate the same function at the molecular level, they exhibit different expression levels and regulation in plant organs and in response to environmental and hormonal signals.

show abstract

Section: Vvgpt1 Is Likely Involved In Starch Accumulation In Heterotrsupporting

confidence: 70%

Identification and functional characterization of grapevine transporters that mediate glucose-6-phosphate uptake into plastids

Noronha

Conde

Delrot

et al. 2015

Planta

View full text Add to dashboard Cite

show abstract

“…A number of elicitors including UV, methyl jasmonate, and indanoyl-isoleucine triggered the production of secondary metabolites, including resveratrol 10, 13, 14, 15, 16; however, the roles of many other potential elicitors remain to be investigated. If secondary metabolites are secreted, in situ adsorption is considered.…”

Section: Introductionmentioning

confidence: 99%

Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture

Vuong

Franco

Zhang

2014

Biotechnology Reports

View full text Add to dashboard Cite

Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in Vitis vinifera L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for in situ removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert.

show abstract

“…Use of this method reduces the time to attain a high yield of product and increases the yield of secondary compounds (Kang et al 2006;Guo et al 2011;Zhao et al 2013). Elicitor is a substance which initiates and/or improves the biosynthesis of bioactive compounds found at a low concentration in a living cell system (Santamaria et al 2011) and classified as biotic or abiotic. Biotic elicitors such as enzymes, fungi or bacteria originate from plant cells and microorganisms have been used in Ginkgo cell cultures for production of flavonoids (Hao et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of varying NaCl doses on flavonoid production in suspension cells of Ginkgo biloba: relationship to chlorophyll fluorescence, ion homeostasis, antioxidant system and ultrastructure

et al. 2014

View full text Add to dashboard Cite

Ginkgo suspension cells were used to investigate the mechanism that governs the shift between primary and secondary metabolism under NaCl elicitation. The production of three flavonol glycosides, chlorophyll fluorescence, ion content, the antioxidant system, and the cellular ultrastructure in the presence of NaCl doses from 5 to 175 mM were examined. At low salt doses (5-50 mM), cell growth and flavonol glycosides accumulation were stimulated without damaging cell structure or inducing oxidative stress by maintaining high K ? and chlorophyll content. At moderate salt doses (75-125 mM), the cells could withstand the salt stress without an impact on survival by changing internal cellular structure, maintaining high levels of K ? and Ca 2? and increasing anti-oxidative enzyme activities rather than flavonol glycosides to counteract the inhibition of the photosystem II, the accumula

show abstract

Effects of Elicitors on the Production of Resveratrol and Viniferins in Cell Cultures of Vitis vinifera L. cv Italia

Cited by 73 publications

References 48 publications

Identification and functional characterization of grapevine transporters that mediate glucose-6-phosphate uptake into plastids

Identification and functional characterization of grapevine transporters that mediate glucose-6-phosphate uptake into plastids

Treatment strategies for high resveratrol induction in Vitis vinifera L. cell suspension culture

Effect of varying NaCl doses on flavonoid production in suspension cells of Ginkgo biloba: relationship to chlorophyll fluorescence, ion homeostasis, antioxidant system and ultrastructure

Contact Info

Product

Resources

About