Effects of Enamel Matrix Derivative on Vascular Endothelial Growth Factor Expression and Microvessel Density in Gingival Tissues of Periodontal Pocket: A Comparative Study

Aspriello, Simone Domenico; Zizzi, Antonio; Spazzafumo, Liana; Rubini, Corrado; Lorenzi, Teresa; Marzioni, Daniela; Bullón, Pedro; Piemontese, Matteo

doi:10.1902/jop.2010.100180

Cited by 32 publications

(31 citation statements)

References 31 publications

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“…Numerous investigations have elaborated on the potential for EMD to induce angiogenesis both in vitro and in animal models 32‐37 . Results from Sakoda et al demonstrated that EMD stimulates VEGF production partially via transforming growth factor‐β1 and fibroblast growth factor 2 in human gingival fibroblasts and that EMD‐induced VEGF production is regulated by extracellular regulated kinase, p38 mitogen‐activated protein kinase, and phosphatidylinositol 3 kinase/Akt pathways 35 .…”

Section: Discussionmentioning

confidence: 99%

Effect of Enamel Matrix Derivative on Periodontal Wound Healing and Regeneration in an Osteoporotic Model

Miron

Wei

Yang

et al. 2014

Journal of Periodontology

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Section: Discussionmentioning

confidence: 99%

Effect of Enamel Matrix Derivative on Periodontal Wound Healing and Regeneration in an Osteoporotic Model

Miron

Wei

Yang

et al. 2014

Journal of Periodontology

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“…Several studies (13–16) have reported that the vascular endothelial growth factor (VEGF) plays an evident role in wound repair. In a study (13) on diabetic mice, impaired wound healing capacity was successfully corrected by administering the VEGF gene to the wounds.…”

Section: Discussionmentioning

confidence: 99%

Effect of enamel matrix derivative protein on the healing of standardized epithelial wounds: a histomorphometric analysis in vivo

Al‐Hezaimi

Al‐Askar

Al-Fahad

et al. 2011

International Wound Journal

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The enamel matrix derivative (EMD) is a preparation of the enamel matrix proteins secreted by the Hertwig's epithelial root sheath. It has been shown that EMD promotes periodontal wound healing; however, the significance of the protein in repairing skin wounds is insufficiently addressed. The aim of this in vivo histomorphometric investigation was to analyse the effect of EMD protein on the healing of standardised epithelial wounds. Dorsal skin of 22-week-old female guinea pigs (n = 33) was scarified and divided into test- (topical application of EMD) and control-sites (sutured and allowed to heal). Animals were euthanised at specific time intervals and the specimens were then evaluated histomorphometrically. The mean widths of the external wound gaps (WGs) in the test- and control-sites at the 5th, 20th and 35th day of healing were 5·89, 3·6 and 1·01 mm and 6·41, 5·02 and 3·43 mm, respectively. Histomorphometric analysis showed a statistically significant difference in the WGs between the test- and control-sites. A significant increase in the formation of organised connective tissue matrix, collagen fibres and early muscle formation was observed in the test-sites as compared with the control-sites. Within the limits of this study, it is concluded that topical application of the EMD on standardised epithelial allows early wound closure and promotes healing as compared to when the defects are merely sutured.

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“…Enamel matrix derivative (EMD) is a complex of low-molecular weight hydrophobic enamel proteins isolated from developing porcine tooth buds and consists by approximately 90% of amelogenin. An ability of EMD to stimulate angiogenesis in vivo was shown by both clinical and animal studies 11,12) . In vitro studies show that EMD possesses chemotactic ability, stimulates ECs migration, formation of vessel like structures, and increase the expression of angiogenesis-related genes [13][14][15][16] .…”

Section: Introductionmentioning

confidence: 98%

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

et al. 2017

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Angiogenesis play a crucial role in the regeneration of hard and soft tissue around dental titanium (Ti) implant. Enamel matrix derivative (EMD) promotes tissue regeneration and stimulates angiogenesis but its effect on the angiogenesis on Ti surfaces was never investigated. The effect of EMD on the angiogenic activity of endothelial cells cultured on pre-treated smooth Ti (PT), acidetched (A), coarse-grit blasted and acid-etched (SLA) surfaces and tissue culture plastic (TCP) in the presence or absence of EMD was investigated. EMD inhibited the proliferation/viability of human umbilical vein endothelial cells (HUVECs) growing on A and SLA Ti surfaces. EMD induced an increase in the expression of all these genes in HUVECs grown on SLA surface but not on other surfaces. Summarizing, our data show that EMD influences proliferation and expression of angiogenesis associated gene in HUVECs grown on moderately rough SLA surfaces, suggesting that EMD might promote angiogenesis following implantation.

show abstract

Effects of Enamel Matrix Derivative on Vascular Endothelial Growth Factor Expression and Microvessel Density in Gingival Tissues of Periodontal Pocket: A Comparative Study

Cited by 32 publications

References 31 publications

Effect of Enamel Matrix Derivative on Periodontal Wound Healing and Regeneration in an Osteoporotic Model

Effect of Enamel Matrix Derivative on Periodontal Wound Healing and Regeneration in an Osteoporotic Model

Effect of enamel matrix derivative protein on the healing of standardized epithelial wounds: a histomorphometric analysis in vivo

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

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