Effects of enamel matrix derivative on proliferation and differentiation of primary osteoblasts

Hägewald, Stefan; Pischon, Nicole; Jawor, Przemyslaw; Bernimoulin, Jean‐Pierre; Zimmermann, Bernd

doi:10.1016/j.tripleo.2004.02.063

Cited by 40 publications

(47 citation statements)

References 35 publications

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“…EMD promotes ALP activity in osteoblasts [33] and C2C12 cells [29]. However, Guida et al have suggested that EMD inhibits ALP activity in human bone marrow stromal cells [30].…”

Section: Resultsmentioning

confidence: 99%

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Katayama

Kato

Taguchi

et al. 2014

IJMS

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Enamel matrix derivative (EMD) is widely used in periodontal tissue regeneration therapy. However, because the bioactivity of EMD varies from batch to batch, and the use of a synthetic peptide could avoid use from an animal source, a completely synthetic peptide (SP) containing the active component of EMD would be useful. In this study an oligopeptide synthesized derived from EMD was evaluated for whether it contributes to periodontal tissue regeneration. We investigated the effects of the SP on cell proliferation and osteoblast differentiation of human mesenchymal stem cells (MSCs), which are involved in tissue regeneration. MSCs were treated with SP (0 to 1000 ng/mL), to determine the optimal concentration. We examined the effects of SP on cell proliferation and osteoblastic differentiation indicators such as alkaline phosphatase activity, the production of procollagen type 1 C-peptide and osteocalcin, and on mineralization. Additionally, we investigated the role of extracellular signal-related kinases (ERK) in cell proliferation and osteoblastic differentiation induced by SP. Our results suggest that SP promotes these processes in human MSCs, and that ERK inhibitors suppress these effects. In conclusion, SP promotes cell proliferation and osteoblastic differentiation of human MSCs, probably through the ERK pathway.

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“…EMD promotes ALP activity in osteoblasts [33] and C2C12 cells [29]. However, Guida et al have suggested that EMD inhibits ALP activity in human bone marrow stromal cells [30].…”

Section: Resultsmentioning

confidence: 99%

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Katayama

Kato

Taguchi

et al. 2014

IJMS

View full text Add to dashboard Cite

show abstract

“…However, findings from in vitro and in vivo experiments indicate that EMD may also influence healing/regeneration of non-tooth related bone defects. In vitro studies with human, rat and mouse osteoblasts showed increased proliferation and/or differentiation in the presence of EMD [30], [35], [36], [37], [38]. In vivo treatment of perforated rat femurs with EMD significantly increased newly formed bone in 7 days when compared to untreated perforated femurs [39].…”

Section: Introductionmentioning

confidence: 95%

Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin

et al. 2011

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In recent years, enamel matrix derivative (EMD) has garnered much interest in the dental field for its apparent bioactivity that stimulates regeneration of periodontal tissues including periodontal ligament, cementum and alveolar bone. Despite its widespread use, the underlying cellular mechanisms remain unclear and an understanding of its biological interactions could identify new strategies for tissue engineering. Previous in vitro research has demonstrated that EMD promotes premature osteoblast clustering at early time points. The aim of the present study was to evaluate the influence of cell clustering on vital osteoblast cell-cell communication and adhesion molecules, connexin 43 (cx43) and N-cadherin (N-cad) as assessed by immunofluorescence imaging, real-time PCR and Western blot analysis. In addition, differentiation markers of osteoblasts were quantified using alkaline phosphatase, osteocalcin and von Kossa staining. EMD significantly increased the expression of connexin 43 and N-cadherin at early time points ranging from 2 to 5 days. Protein expression was localized to cell membranes when compared to control groups. Alkaline phosphatase activity was also significantly increased on EMD-coated samples at 3, 5 and 7 days post seeding. Interestingly, higher activity was localized to cell cluster regions. There was a 3 fold increase in osteocalcin and bone sialoprotein mRNA levels for osteoblasts cultured on EMD-coated culture dishes. Moreover, EMD significantly increased extracellular mineral deposition in cell clusters as assessed through von Kossa staining at 5, 7, 10 and 14 days post seeding. We conclude that EMD up-regulates the expression of vital osteoblast cell-cell communication and adhesion molecules, which enhances the differentiation and mineralization activity of osteoblasts. These findings provide further support for the clinical evidence that EMD increases the speed and quality of new bone formation in vivo.

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“…It was also suggested that EMD may elicit its mitogenic signal through an EMD-specific receptor tyrosine kinase towards extracellular signal-regulated kinase 1/2 [36] . It seems that EMD treatment may enhance the cellular activities of osteoblasts and osteoclasts, which in turn might support the regeneration of periodontal bony defects [37] . Since soluble peptides released from EMD may contribute to the stimulating effects on cell proliferation, a direct contact between EMD and osteoblasts might not be required to induce cell proliferation [38] .…”

Section: In Vitro Studiesmentioning

confidence: 99%

The Application of an Enamel Matrix Protein Derivative (Emdogain®) in Regenerative Periodontal Therapy: A Review

et al. 2007

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Regenerative periodontal therapy aims at reconstitution of the lost periodontal structures such as new formation of root cementum, periodontal ligament and alveolar bone. Findings from basic research indicate that enamel matrix protein derivative (EMD) has a key role in periodontal wound healing. Histological results from animal and human studies have shown that treatment with EMD promotes periodontal regeneration. Moreover, clinical studies have indicated that treatment with EMD positively influences periodontal wound healing in humans. This review aims to present an overview of evidence-based clinical indications for regenerative therapy with EMD.

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Effects of enamel matrix derivative on proliferation and differentiation of primary osteoblasts

Cited by 40 publications

References 35 publications

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

The Effects of Synthetic Oligopeptide Derived from Enamel Matrix Derivative on Cell Proliferation and Osteoblastic Differentiation of Human Mesenchymal Stem Cells

Premature Osteoblast Clustering by Enamel Matrix Proteins Induces Osteoblast Differentiation through Up-Regulation of Connexin 43 and N-Cadherin

The Application of an Enamel Matrix Protein Derivative (Emdogain®) in Regenerative Periodontal Therapy: A Review

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