Effects of endocrine disrupting chemicals on the expression of CYP19 genes in zebrafish (Danio rerio) juveniles

Kazeto, Yukinori; Place, Allen R.; Trant, John M.

doi:10.1016/j.aquatox.2004.04.008

Cited by 202 publications

(125 citation statements)

References 33 publications

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“…In zebra fish (Danio rerio) exposed up to 3 µg/L BaP for 56 days, CYP19A2 mRNA was increased but no effect on CYP19A1 was observed (Hoffman & Oris, 2006). Similarly, CYP19A2 induction was found when 17 dpf juvenile zebra fish were exposed for 3 days to 10 µM BaP (2.5 mg/L) (Kazeto et al, 2004). In contrast, in our previous studies with Fundulus exposed to water borne BaP up to 10 µg/L for 15 days, we did not see a significant effect on expression of either CYP19 message (Patel et al, 2006).…”

Section: Genotoxicity Due To Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 44%

Scenario of Genotoxicity in Fishes and Its Impact on Fish Industry

Mir¹,

Khan²,

Bhat³

et al. 2014

IOSRJESTFT

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Section: Genotoxicity Due To Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 44%

Scenario of Genotoxicity in Fishes and Its Impact on Fish Industry

Mir¹,

Khan²,

Bhat³

et al. 2014

IOSRJESTFT

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“…increased the expression of aromatase (Cytochrome P450-19; CYP19) in fish (Min et al 2003, Kazeto et al 2004 but not in mammalian models (Odum et al 2001). These observations suggest that NP-induced alterations in steroid synthesis, metabolism, and clearance, in addition to direct activation of ERa contribute to the estrogenic effects of this compound in fish.…”

Section: Regulation Of Lmb Er Isotype and Vtg1 Expression By Np In Vivomentioning

confidence: 70%

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

Sabo‐Attwood

Blum

Kroll

et al. 2007

Journal of Molecular Endocrinology

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The estrogen receptor (ER) signaling cascade is a vulnerable target of exposure to environmental xenoestrogens, like nonylphenol (NP), which are causally associated with impaired health status. However, the impact of xenoestrogens on the individual receptor isotypes (a, ba, and bb) is not well understood. The goal of these studies was to determine the impact of NP on largemouth bass (Micropterus salmoides) ER isotype expression and activity. Here, we show that hepatic expression levels of three receptors are not equivalent in male largemouth bass exposed to NP by injection. Transcript levels of the ERa subtype were predominantly induced in concert with vitellogenin similarly to fish exposed to 17b-estradiol (E 2 ) as measured by quantitative real-time PCR. NP also induced circulating plasma levels of estrogen, which may contribute to overall activation of the ERs. To measure the activation of each receptor isotype by E 2 and NP, we employed reporter assays using an estrogen response element (ERE)-luciferase construct. Results from these studies show that ERa had the greatest activity following exposure to E 2 and NP. This activity was inhibited by the antagonists ICI 182 780 and ZM 189 154. Furthermore, both bb and ba subtypes depressed ERa activation, suggesting that the cellular composition of receptor isotypes may contribute to the overall actions of estrogen and estrogenic contaminants via the receptors. Results from these studies collectively reveal the differential response of fish ER isotypes in response to xenoestrogens.

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“…In addition, brain cyp19b mRNA levels were generally 10 times higher than cyp19a levels (Lyssimachou et al, 2006). Thus, the lack of EDC effects on testicular cyp19 expression may be related to the differential tissue abundance and expression of the cyp19 genes after exposure to estrogenic compounds that have been reported previously Kazeto et al, 2004;Lyssimachou et al, 2006;Meucci and Arukwe, 2006) or transient effect that was not captured by the timing of sampling or sex-related effects (i.e. male vs. female).…”

Section: Effects On Steroidogenic Enzyme Genesmentioning

confidence: 84%

The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP)

Bello

Madekurozwa

Groenewald

et al. 2014

Comparative Biochemistry and Physiology Part C: Toxicology &

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Keywords:Di-(n-butyl) phthalate Leydig cell Steroidogenesis Male Japanese quails Endocrine disruptionIn the present study, we have investigated the effects of 30-day dietary (pre-pubertal) exposure to different doses (0 (control), 1, 10, 50, 200 and 400 mg/kg bodyweight/day) of di(n-butyl) phthalate (DBP) on Leydig cells of adult male Japanese quails by quantifying the transcript levels for P450 side-chain cleavage (p450scc), P450c17 (CYP17), and 3β-and 17β-hydroxysteroid dehydrogenase (hsd) using quantitative (real-time) poly-merase chain reaction (qRT-PCR). In addition, the plasma testosterone levels were analysed using radioimmuno-assay (RIA) and testis was examined for evidence of gross pathology and histopathology. Our data showed that pre-pubertal exposure to DBP produced alterations in testicular architecture as evident by poorly developed or misshaped testis, and altered spermatogenesis due to tubular degeneration and atrophy of seminiferous tubules especially in the high DBP dose (200 and 400 mg/ kg) treated groups. In addition, DBP altered several key en-zymes involved in testicular steroidogenesis pathways in an apparent dose-dependent manner. For example, bi-phasic effects of DBP were observed for P450scc and 3β-hsd mRNA, that were generally increasing at low dose 10 mg/kg, and thereafter, an apparent dose-dependent decrease between 50 and 400 mg/kg. The steroidogenic acute regulatory (StAR) protein was at the lowest detectable limits and therefore not quantifiable. These effects did not parallel the non-significant changes observed for plasma testosterone levels. The present data is consis-tent with previous reports showing that DBP modulates Leydig cell steroidogenesis in several species, with a po-tential negative effect on reproduction in those avian species that are vulnerable to endocrine disrupting chemicals.

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Effects of endocrine disrupting chemicals on the expression of CYP19 genes in zebrafish (Danio rerio) juveniles

Cited by 202 publications

References 33 publications

Scenario of Genotoxicity in Fishes and Its Impact on Fish Industry

Scenario of Genotoxicity in Fishes and Its Impact on Fish Industry

Distinct expression and activity profiles of largemouth bass (Micropterus salmoides) estrogen receptors in response to estradiol and nonylphenol

The effects on steroidogenesis and histopathology of adult male Japanese quails (Coturnix coturnix japonica) testis following pre-pubertal exposure to di(n-butyl) phthalate (DBP)

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