“…16 Zeta potential of molasses CDs was measured as negative (− 5.43 mV at pH 5.09 and − 12.9 mV at pH 8.67) at room temperature (25 °C). 16 Silica coating is one of the simple way to obtain quantum dots with different properties like water solubility, moderate buffer stability and photo stability. 17 were plotted (Fig.…”
Dopamine (DA) is an important electroactive neurotransmitter. The concentration of DA in the body of a healthy person is approximately 1.0x10-7-1.0x10-3 M. A decrease in dopamine concentration is associated with Parkinson’s disease. Thus, it is important to determine the amount of dopamine in early diagnosis of Parkinson’s disease. Different methods such as immunoassay, flow injection analysis (FIA), high performance liquid chromatography (HPLC) etc. could be used for determination of dopamine but they are expensive and have long determination times, and pre analytic processes. In this study, a tyrosinase based amperometric biosensor was developed with carbon paste electrode modified with carbon nano dot 3-Chloropropyl-trimethoxysilane (CDs-CPTMS) for determination of the amount of dopamine. CDs-CPTMS was synthesized for the first time. Determination of dopamine was carried out by the reduction of dopamine-o-quinone at -0.15 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the dopamine response of the prepared biosensor and interference effect were investigated. There was no interference effect of uric acid and ascorbic acid. The designed biosensor has wide working range (0.001- 0.01μM and 0.01- 0.1 μM), low limit of detection, very good reproducibility and shelf life. In addition, the preparation of the biosensor is practical and cost-effective.
“…16 Zeta potential of molasses CDs was measured as negative (− 5.43 mV at pH 5.09 and − 12.9 mV at pH 8.67) at room temperature (25 °C). 16 Silica coating is one of the simple way to obtain quantum dots with different properties like water solubility, moderate buffer stability and photo stability. 17 were plotted (Fig.…”
Dopamine (DA) is an important electroactive neurotransmitter. The concentration of DA in the body of a healthy person is approximately 1.0x10-7-1.0x10-3 M. A decrease in dopamine concentration is associated with Parkinson’s disease. Thus, it is important to determine the amount of dopamine in early diagnosis of Parkinson’s disease. Different methods such as immunoassay, flow injection analysis (FIA), high performance liquid chromatography (HPLC) etc. could be used for determination of dopamine but they are expensive and have long determination times, and pre analytic processes. In this study, a tyrosinase based amperometric biosensor was developed with carbon paste electrode modified with carbon nano dot 3-Chloropropyl-trimethoxysilane (CDs-CPTMS) for determination of the amount of dopamine. CDs-CPTMS was synthesized for the first time. Determination of dopamine was carried out by the reduction of dopamine-o-quinone at -0.15 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the dopamine response of the prepared biosensor and interference effect were investigated. There was no interference effect of uric acid and ascorbic acid. The designed biosensor has wide working range (0.001- 0.01μM and 0.01- 0.1 μM), low limit of detection, very good reproducibility and shelf life. In addition, the preparation of the biosensor is practical and cost-effective.
“…6, CDs gave a broad peak centered at ca. 20° (2θ), which indicates the amorphous structure of CDs [8].…”
Section: Resultsmentioning
confidence: 99%
“…Morphologies of molasses CDs were characterized by using a transmission electron microscope (TEM), selected area electron diffraction (SAED) ,and X‐ray diffraction (XRD) analysis [8]. …”
Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot‐(3‐Aminopropyl) triethoxysilane (CDs‐APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross‐linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs‐APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.
“…Innumerable food-containing carbon sources can be employed to synthesize CDs with different QYs, e.g., yoghurt, honey, banana, pomegranate, leaves, sugar beet molasses, egg, rice bran, garlic, coffee beans, soybeans, coconut shell, tea leaves, grass, etc. [93][94][95][96][97][98][99][100][101]. Additionally, various green approaches for one-step synthesis of fluorescent CDs can be obtained using natural or synthetic non-toxic precursors for specific biosensing purposes, e.g., wool for synthesizing CDs to detect glycophosphate [102], sodium fluoride functionalized chitosan for preparing CDs to determine the retinoic acid content [103], etc.…”
Carbon dots (CDs) are usually smaller than 10 nm in size, and are meticulously formulated and recently introduced nanomaterials, among the other types of carbon-based nanomaterials. They have gained significant attention and an incredible interest in the field of nanotechnology and biomedical science, which is merely due to their considerable and exclusive attributes; including their enhanced electron transferability, photobleaching and photo-blinking effects, high photoluminescent quantum yield, fluorescence property, resistance to photo-decomposition, increased electrocatalytic activity, good aqueous solubility, excellent biocompatibility, long-term chemical stability, cost-effectiveness, negligible toxicity, and acquaintance of large effective surface area-to-volume ratio. CDs can be readily functionalized owing to the abundant functional groups on their surfaces, and they also exhibit remarkable sensing features such as specific, selective, and multiplex detectability. In addition, the physico-chemical characteristics of CDs can be easily tunable based on their intended usage or application. In this comprehensive review article, we mainly discuss the classification of CDs, their ideal properties, their general synthesis approaches, and primary characterization techniques. More importantly, we update the readers about the recent trends of CDs in health care applications (viz., their substantial and prominent role in the area of electrochemical and optical biosensing, bioimaging, drug/gene delivery, as well as in photodynamic/photothermal therapy).
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