Effects of Enterobacter cloacae on boar sperm quality during liquid storage at 17°C

Prieto-Martínez, Noelia; Bussalleu, E; Garcia-Bonavila, Estela; Bonet, Sergi; Yeste, Marc

doi:10.1016/j.anireprosci.2014.05.008

Cited by 62 publications

(37 citation statements)

References 36 publications

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“…The electrode was thoroughly washed every time with distilled water before and after sample detection. 3–5 replicates were assessed for every sample47.…”

Section: Methodsmentioning

confidence: 99%

Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways

Zhao

Zhang

Liu

et al. 2016

Sci Rep

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A number of emerging studies suggest that air pollutants such as hydrogen sulfide (H2S) and ammonia (NH3) may cause a decline in spermatozoa motility. The impact and underlying mechanisms are currently unknown. Boar spermatozoa (in vitro) and peripubertal male mice (in vivo) were exposed to H2S and/or NH3 to evaluate the impact on spermatozoa motility. Na2S and/or NH4Cl reduced the motility of boar spermatozoa in vitro. Na2S and/or NH4Cl disrupted multiple signaling pathways including decreasing Na+/K+ ATPase activity and protein kinase B (AKT) levels, activating Adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) and phosphatase and tensin homolog deleted on chromosome ten (PTEN), and increasing reactive oxygen species (ROS) to diminish boar spermatozoa motility. The increase in ROS might have activated PTEN, which in turn diminished AKT activation. The ATP deficiency (indicated by reduction in Na+/K+ ATPase activity), transforming growth factor (TGFβ) activated kinase-1 (TAK1) activation, and AKT deactivation stimulated AMPK, which caused a decline in boar spermatozoa motility. Simultaneously, the deactivation of AKT might play some role in the reduction of boar spermatozoa motility. Furthermore, Na2S and/or NH4Cl declined the motility of mouse spermatozoa without affecting mouse body weight gain in vivo. Findings of the present study suggest that H2S and/or NH3 are adversely associated with spermatozoa motility.

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“…The electrode was thoroughly washed every time with distilled water before and after sample detection. 3–5 replicates were assessed for every sample47.…”

Section: Methodsmentioning

confidence: 99%

Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways

Zhao

Zhang

Liu

et al. 2016

Sci Rep

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“…Microorganisms can cause serious economic damages by reducing the semen quality and possible dissemination of pathogens (Prieto-Martínez et al, 2014). Contaminated semen can reduce the conception rate, cause early embryonic death and/or endometritis, clinical diseases in herds, and/or infections by unwanted pathogens (Maes et al, 2008).…”

Section: Sources and Consequences Of Bacterial Contamination In Semenmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

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How to cite: Santos CS, Silva AR. Current and alternative trends in antibacterial agents used in mammalian semen technology. AbstractThe use of antibacterial substances as additives in extenders for ensuring the sanitary quality of the semen employed in reproductive biotechniques and preserving it from bacterial deterioration has been reported since the mid-twentieth century. However, the deleterious effects of these drugs on the sperm quality as well as their effectiveness in controlling bacterial growth in the preserved semen have been questioned. The aim of this review was to report the antimicrobials primarily used in the extenders added to the semen of mammals, and to present alternatives to their use. Among the various mammalian species, there is a large variation regarding the antimicrobial types added to semen extenders as cephalosporins (ceftiofur, cefdinir, eg) and quinolones (ofloxacin, ciprofloxacin), alone or in combination with large action spectra substances as penicillin-streptomycin and gentamicin-tylosin-lincomycin-spectinomycin. To combat problems related to bacterial resistance to these drugs, the emergence of alternatives is increasingly evident. Among these alternatives, use of physical methods as centrifugation and filtration, as well as the use of antimicrobial peptides and other substances from different origins have been highlighted for presenting antimicrobial potential.

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“…Our results are thus in agreement with the numerous studies that indicate that bacterial contamination can affect the quality and longevity of boar semen during storage, and that high levels of bacterial contamination can lead to undesired effects in insemination doses and fertility results (Althouse et al 2000, Maroto Martín et al 2010, Bussalleu et al 2011, Sa et al 2014, especially when the proportion of sperm to bacteria is approximately 1:1, or for some types of bacteria, 100:1 (Althouse 2008, Althouse et al 2008, Kuster and Althouse 2016. The negative impact of bacteria on boar semen stored at temperatures of [15][16][17] o C (after a little more than 24-48 h from inoculation) was indicated mainly by experimental studies that showed high concentrations of some bacteria species (Bussalleu et al 2011, Sepúlveda et al 2013, Prieto-Martínez et al 2014.…”

Section: Response To Storage Of Bacterial Growth Dynamics and Sperm Qmentioning

confidence: 99%

Bacterial Contamination of Boar Semen and its Relationship to Sperm Quality Preserved in Commercial Extender Containing Gentamicin Sulfate

Gączarzewicz¹,

Udała²,

Piasecka³

et al. 2016

Polish Journal of Veterinary Sciences

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This study was designed to determine the degree and type of bacterial contamination in boar semen (79 ejaculates from Large White and Landrace boars) and its consequences for sperm quality during storage (27 extended semen samples, 16 o C for five days) under practical conditions of artificial insemination (AI). The results revealed the presence of aerobic bacteria in 99% of the ejaculates (from 80 to 370 ×10 6 colony-forming units/mL). Most of the ejaculates contained two or three bacterial contaminants, while the Staphylococcus, Streptococcus, and Pseudomonas bacterial genera were most frequently isolated. Also detected were Enterobacter spp., Bacillus spp., Proteus spp., Escherichia coli, P. fluorescens, and P. aeruginosa. In general, the growth of certain bacterial types isolated prior to semen processing (Enterobacter spp., E. coli, P. fluorescens, and P. aeruginosa) was not discovered on different days of storage, but fluctuations (with a tendency towards increases) were found in the frequencies of Bacillus spp., Pseudomonas spp., and Staphylococcus spp. isolates up to the end of storage. Semen preserved for five days exhibited decreases in sperm motility and increases in the average number of total aerobic bacteria; this was associated with sperm agglutination, plasma membrane disruption, and acrosome damage. We inferred that, due to the different degrees and types of bacterial contaminants in the boar ejaculates, the inhibitory activity of some antimicrobial agents used in swine extenders (such as gentamicin sulfate) may be limited. Because such agents can contribute to the overgrowth of certain aerobic bacteria and a reduction in the quality of stored semen, procedures with high standards of hygiene and microbiological control should be used when processing boar semen.

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Effects of Enterobacter cloacae on boar sperm quality during liquid storage at 17°C

Cited by 62 publications

References 36 publications

Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways

Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways

Current and alternative trends in antibacterial agents used in mammalian semen technology

Bacterial Contamination of Boar Semen and its Relationship to Sperm Quality Preserved in Commercial Extender Containing Gentamicin Sulfate

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